Recombinant Rat Transforming growth factor beta-1 proprotein(Tgfb1),partial

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Code CSB-EP023446RAa0
Size US$2466
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names Tgfb1
Uniprot No. P17246
Research Area Signal Transduction
Alternative Names Tgfb1; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide; LAP); Transforming growth factor beta-1; TGF-beta-1)]
Species Rattus norvegicus (Rat)
Source E.coli
Expression Region 30-278aa
Target Protein Sequence LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYDKTKDITHSIYMFFNTSDIREAVPEPPLLSRAELRLQRFKSTVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNVLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 34.0 kDa
Protein Length Partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (By similarity). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (By similarity). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (By similarity).
Gene References into Functions
  1. High TGFB1 expression is associated with glomerular mesangial cell hypertrophy. PMID: 29397070
  2. High TGFB1 expression is associated with liver fibrosis. PMID: 30243650
  3. In the present study, we assessed the effect of boldine on the progression of kidney disease using the 2K1C hypertension model and identifying mediators in the RAS, such as TGF-β, that could be modulated by this alkaloid PMID: 29941815
  4. Ang II induced epithelial-mesenchymal transition in renal tubular epithelial cells via Tgfb1. PMID: 29746862
  5. LET suppression promoted BMSCs proliferation by up-regulating the expression of TGF-beta1 and activating Wnt/beta-catenin pathway. PMID: 29068476
  6. These findings implicate TGFbeta1 as one of the important factors impairing drug induced CYP induction in co-cultures and suggests that caution needs to be exercised in the use of hepatocyte-fibroblast co-cultures for CYP induction studies. PMID: 29366910
  7. This suggests that MG53 is a potential regulator of atrial fibrosis induced by the TGF-beta1/Smad pathway in patients with atrial fibrillation . PMID: 29233682
  8. This suggests that soybean isoflavones could delay the process of renal interstitial fibrosis in DN rats by decreasing the expression of Wnt4, beta-catenin and TGF-beta1 in the renal interstitium, thus demonstrating that soybean isoflavones plus losartan have the best protective effects against diabetes-induced renal fibrosis. PMID: 29921370
  9. The results of our study suggest that miR-142 inhibits proliferation and promotes apoptosis in ASMCs during airway remodeling in asthmatic rats by inhibiting TGF-beta expression via a mechanism involving the EGFR signaling pathway. PMID: 29949788
  10. In cultured hippocampal neurons, TGF-[beta]1 prevented A[beta]1-42-induced APP, TNF-[alpha], Bax, cleaved caspase-3, and cleaved caspase-9 expression upregulation, and cell apoptosis, showing a neuroprotective effect of TGF-[beta]1. Silencing of the T[beta]R1 gene abolished the neuroprotective effect of TGF-[beta]1. PMID: 29200096
  11. Acetyl-11-Keto-beta-Boswellic Acid is able to attenuate oxidative stress and profibrotic mechanisms, and improve vascular remodeling in hypertension through TGF-beta1/Smad3 pathway. PMID: 28009003
  12. the truncated variant of gHGF (a double mutant of N-terminal hairpin and first kringle domains of gHGF, K132E and G134E, gmNK1) protein fused with His6 tag, the molecular weight of which was about 20.0kDa, which was expressed in Escherichia coli BL21 (DE3) and purified with Ni(2+)-affinity chromatography.gmNK1 inhibited protein expression levels of fibrosis-related Col I and alpha-SMA in TGF-beta1-activated HSC-T6 cells PMID: 28837849
  13. The results suggest that the CIP4/Par6 complex plays an important role in the occurrence of epithelial-mesenchymal transition in TGF-beta1-stimulated epithelial kidney cells. PMID: 28848997
  14. calcitonin and TGF-beta have roles in cartilage degeneration and nociception in an experimental rat model of osteoarthritis PMID: 27345362
  15. Suggest a potentially mutual regulation between IGFBPrP1 and TGFbeta1, which activates hepatic stellate cells and extracellular matrix deposition. PMID: 28823370
  16. Study demonstrated that the activation of transforming growth factor-beta1 (TGF-beta1) in mild traumatic brain injury (mTBI) correlated with the induction of free radical generating enzyme NADPH oxidase 1 (NOX1). Study study could establish the significance of TGF-beta1 in transforming the pathophysiology of mTBI into secondary injury. PMID: 28433746
  17. TGF-Beta1 (and Egr-1) are up-regulated by sustained hyperosmolarity in the renal medulla. PMID: 28673338
  18. The positive correlation between Reg1a and Tgfb1 genes expression was determined in the dynamics of stress-induced gastric lesions development and healing, which may indicate the involvement of Tgfb1 in acceleration of lesions healing. PMID: 29537200
  19. In conclusion, PFD and Collagen-PVP decrease inflammation, fibrosis, TGFbeta-1 expression, and CD in the posttracheoplasty rats' scar. PMID: 28584818
  20. TGFB1-expressing mesenchymal stem cells induce local tolerance in a rat liver transplantation model of acute rejection. PMID: 27333806
  21. In the present study, isolated rat peritoneal mesothelial cells were treated with TGF-beta1 and/or IL-6 which elicited mesenchymal transition (MMT) as determined by morphologic and biochemical techniques ..Partial characterization of relevant signaling pathways identified Erk1/2 activation, which was enhanced by combined TGF-b1/IL-6 administration, as a crucial necessary factor in the transition.. PMID: 26825317
  22. miR-497 could suppress TGFbeta1-induced pulmonary fibroblast proliferation and EIF3A, Collagen I and alpha-SMA protein levels. PMID: 28322466
  23. could prevent cardiac fibroblasts from activation into myofibroblast and protect HUVEC against endothelial to mesenchymal transition. These effects may be mediated by inhibition of the TGFbeta pathway in both cardiac fibroblasts and HUVECs. PMID: 28303407
  24. Administration of Methylglyoxal led to depletion of antioxidant enzymes, induction of fibrosis (p < 0.001), up-regulated expression of RAGE (3.5 fold), TGF-beta (4.4 fold), SMAD2 (3.7 fold), SMAD3 (6.0 fold), IL-6 (4.3 fold) and TNF-alpha (5.5 fold) in the heart tissues compared to control rats. PMID: 28091942
  25. High TGFB1 expression is associated with pulmonary arterial hypertension. PMID: 29069575
  26. Data suggest that white adipose tissues in various sites respond differently to distinct diet patterns regarding Tgfb expression and signaling pathways; here, energy-restricted and fat-restricted diet containing fish oil down-regulates expression of Tgfb in mesenteric adipose tissue and up-regulates expression of Tgfb in retroperitoneal adipose tissue. PMID: 27736730
  27. Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-beta-mediated Nox4 expression. PMID: 28063381
  28. Transforming growth factor beta 1 overexpression observably activated these two pathways, suggesting that Transforming growth factor beta 1 might participate in the modulation of vitamin D through regulating ERK and JNK pathways in Bone marrow-derived mesenchymal stem cells. PMID: 28817810
  29. The data suggest that miR-155 plays a role in mediating TGF-beta1-induced podocyte injury via nephrin, desmin and caspase-9. PMID: 28719898
  30. This paper provides evidence that TGF-beta stimulates prolonged EGFR transactivation, which is dependent on c-Src tyrosine kinase (Src). PMID: 28943431
  31. Although TGF-beta promotes circulating regulatory T cells (Tregs)in female spontaneously hypertensive rats (SHR), it does not account for the sex difference in renal Tregs in SHR. PMID: 28679591
  32. Mesenchymal stem cells could inhibit the process of chronic rejection of small intestine transplant. The mechanisms may include the inhibited function of these cells on lymphocyte proliferation, reduced infiltration of macrophages, and reduced expression of transforming growth factor beta1 and platelet-derived growth factor C. PMID: 28338461
  33. this study shows that FTY720 attenuates renal fibrosis by TGF-beta signaling in fibroblasts PMID: 28658504
  34. Apigenin ameliorated renal damage due to diabetic nephropathy by suppressing oxidative stress and fibrosis via MAPK-NF-kappaB-TNF-alpha and TGF-beta1-MAPK-fibronectin pathways. PMID: 28566504
  35. High glucose stress suppresses SnoN while activating TGF-beta1 and Rnf111 (Arkadia) in renal tubular epithelial cells and diabetic rats. PMID: 28350874
  36. The fibromodulin (Fm)-transforming growth factor (TGF)-beta1 nexus plays a crucial in fetal-type scarless skin repair. PMID: 27665369
  37. mRNA/protein levels are increased in kidney remnants from 5/6 nephrectomized rats and strongly correlate with TGF-beta1 expression. PMID: 27714812
  38. Data indicate transforming growth factor beta (TGFbeta) involvement in early activation of platelet-derived growth factor receptor beta (PDGFRbeta)+ pericytes in aristolochic acid induced acute kidney injury. PMID: 27379382
  39. TGFbeta1 is involved in cholangiocarcinoma tumor progression and participates through miR-34a mediated downstream signaling cascades. PMID: 27364974
  40. In high glucose-treated glomerular mesangial cells, TGR5 can suppress the NF-kappaB-mediated upregulation of fibronectin and TGF-beta1 by inhibiting RhoA/ROCK signaling via protein kinase A. PMID: 27470217
  41. this study shows that TGF-beta1 vaccine attenuated diabetic nephropathy in OLETF rats through reduction of inflammation, improvement of kidney fibrosis and partial correction of glucose metabolism PMID: 27485077
  42. No differences in fiber arrangement, structure, and nuclei form were noted in either group (P > 0.05). Our findings suggest that TGF-beta1 signaling is necessary for the regulation of tendon cross-link formation, as well as collagen and LOX gene transcription in an exercise-dependent manner. PMID: 27927626
  43. Overexpression of IL-6 is sufficient to induce myofibroblastic proliferation, differentiation, and fibrosis, probably via increased TGF-beta1-mediated MMP2/MMP3 signaling during ischemic myocardial remodeling monoculture or coculture models under hypoxic conditions. PMID: 27348628
  44. TGFbeta1 specifically elevates the expression level of TRPC6 mainly through Smad-dependent canonical pathway. The siRNA against TRPC6 abolished TGFbeta1-induced molecular and cellular phenotype changes, including myosin light chain phosphorylation, actin stress fiber formation, and cell migration. PMID: 28039055
  45. in diabetes, retinal vessels become dependent on a small increase in TGF-beta signaling via activin receptor-like kinase 5 to maintain early integrity. PMID: 28162229
  46. the application of TSLP short hairpin RNA caused a stark reversal of the TGF-beta1-induced cellular fibrosis. PMID: 27492484
  47. this study shows that adipose-derived stem cells treatment attenuates renal interstitial fibrosis possibly through inhibition of epithelial-mesenchymal transition and inflammatory response via TGF-beta1 signaling pathway PMID: 28092863
  48. Alloxan-induced renal damage is associated with alterations of p53, TGF-beta1, and extracellular matrix metalloproteinases. PMID: 28220578
  49. Calcitriol may protect diabetic nephropathy from fibrosis via inhibition of TGF-beta1 and CIP4. PMID: 26097534
  50. The in vitro and in vivo models revealed that lipid metabolism disorder and FK506 caused oxidative stress and a fibrogenic response. In addition, decreased levels of LOX1 markedly reduced the levels of TGFb1 in the in vitro model. PMID: 27633115
  51. posttranslational modification of CREB1 inhibited the profibrotic effects of TGFbeta1 in HSCs via Smaddependent and independent signaling pathways. PMID: 27840939
  52. The results indicated that olmesartan medoxomil significantly decreased collagen deposition and hydroxyproline content, ameliorated pathological score and decreased the elevation in type I collagen and TGF-ss1 mRNA expression in lung tissue. PMID: 26781185
  53. These findings suggest that p-CREB-1 may function as a potent profibrogenic factor through the transactivation of TGF-beta1 expression in liver fibrosis. PMID: 27279449
  54. Paclitaxel at low non-cytotoxic doses ameliorates renal fibrosis by inhibiting multiple steps in the TGF-beta1-induced PAI-1 signaling including Smads and mitogen-activated protein kinases. PMID: 26869361
  55. Naringenin acts as a protective agent in diabetic renal impairment by altering oxidative stress, modulation of cytokine and TGFB1 expression and apoptotic events. PMID: 26928632
  56. the present study demonstrates that a combined treatment with low-dose gefitinib and vitamin E has synergistic effects that reduce TGFbeta1induced fibroblast proliferation, cell-cycle arrest and the ERK phosphorylation pathway. PMID: 27109695
  57. Taken together, these results reveal the key role of histone H3Kme in TGF-beta1 mediated p21 gene expression in RMC, partly through HMT SET7/9 occupancy, suggesting H3Kme and SET7/9 may be potential renoprotective agents in managing chronic renal diseases. PMID: 27247942
  58. Data indicate the effect of angiotensin II receptors' blockade on the macrophage infiltration and histone modifications occurring at the promoter region of monocyte chemoattractant protein (MCP-1) and transforming growth factor-beta (TGF-beta1). PMID: 27693081
  59. AGE are an important factor for cardiac aging and fibrosis, whereas the receptor for AGE and TGF-beta/Smad signaling pathway might be involved in the AGE-induced cardiac aging process. PMID: 26016731
  60. he study provides further evidence that arecanut and pan masala induce oral submucous fibrosisvia the TGF beta1 pathway. PMID: 27072267
  61. the role of the TGF-beta signaling pathway and the downstream c-Jun N-terminal kinase (JNK) signaling pathway in neuroprotective mechanism, was investigated. PMID: 26898453
  62. Atorvastatin reduced Tgfb1 levels in aging rat kidneys. PMID: 26680767
  63. Transforming growth factor-beta1 promotes homing of bone marrow mesenchymal stem cells in renal ischemia-reperfusion injury, which will provide useful data on role of TGF-beta1 in regulating SDF-1/CXCR4 axis-induced MSCs homing PMID: 26722423
  64. Serum and urinary TGFss1 did not show alterations in response to severity and length of urinary obstruction in rats, which might be associated with less intense renal remodeling. PMID: 25560248
  65. Aldosterone at different concentrations exerts synergistic effect with TGF-beta1 to increase PAI-1 expression in hepatic stellate cells PMID: 26617694
  66. finding reveals that Nox2 may promote synthesis of COL I and III via involved in PI3K/AKT and PKC/Rho signaling pathway in a TGF-beta dependent manner and consequently promote cardiac fibrosis PMID: 26617806
  67. TGF-beta-mediated PAI-1 response and increases expression of transcripts associated with cell cycle regulation PMID: 26568079
  68. aberrant activation of TGF-beta in the subchondral bone is involved at the onset of rheumatoid arthritis joint cartilage degeneration. PMID: 25967237
  69. These findings provide novel evidence supporting the anti-catabolic role of TGF-beta1 in IVDD, which is important for the potential clinical application of TGF-beta1 in disc degenerative disorders. PMID: 26075533
  70. Plakophilin-2 loss promotes TGF-beta1/p38 MAPK-dependent fibrotic gene expression in cardiomyocytes. PMID: 26858265
  71. TGFB1 induces a fibrotic phenotype in lung fibroblasts that is counteracted by targeting the proteoglycan synthesis pathway PMID: 26751072
  72. Authors investigated the expression and secretion of lubricin in BMSCs treated with different combinations of TGF-beta1, BMP-7, and/or KGN. PMID: 25857705
  73. TGFBeta1 is involved in neuroprotection and astrogliosis, via activation of Smad3 in the brain after injury. PMID: 26253398
  74. data indicate that there is a prosclerotic feedback loop in mesangial cells dependent on matrix-derived signals in which Hic-5 is a pivotal signaling protein. This feedback loop is TGF-beta-independent PMID: 26405299
  75. These data support the hypothesis that IL-1beta was initially induced by hyperoxia; alphavbeta6 subsequently interacted with and activated TGF-beta1. PMID: 26279426
  76. AFAP1 is an essential downstream signaling component of TGF-beta1 for Src activation, CCN2 induction and collagen XIIa in osteoblasts. PMID: 26340021
  77. This study demonstrated that stretching activated TGF-beta1 gene expressions and increased the proliferation of connective tissue but did not avoid muscle fiber atrophy or induce sarcomerogenesis of denervated rat soleus muscle PMID: 25960249
  78. we have identified a novel TGFbeta1-driven, Smad-independent, signaling cascade that may play an important role in regulating the fibrotic response in activated cardiac myofibroblasts following cardiac injury PMID: 26566727
  79. TGF-beta1 and alpha-SMA expression is reduced in a rat model of hepatic fibrosis treated with antigenic peptide vaccine PMID: 25740471
  80. findings reveal that FoxO1 is regulated by TGF-beta1 and plays a critical role in cardiac fibroblast differentiation. PMID: 26518453
  81. TGF-beta1 exerts anabolic effects on intervertebral discs by inhibiting the expression of syndecan-4. PMID: 25491150
  82. these results suggested that TGF-beta1 and Serpine 1 overexpression might play an important role in DVT formation and have predictive values. PMID: 26535698
  83. miR-7a/b has an anti-fibrotic role in ANG II-treated CFs that is mediated by Sp1 mechanism involving the TGF-beta and MAPKs pathways. PMID: 25923922
  84. Data show that secretion of hepatocyte growth factor (HGF) was elevated, tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta1 (TGF-beta1) levels were decreased after infusion of mesenchymal stem cells. PMID: 25736907
  85. TGF-beta1 expression was significantly reduced inside the callus cells of diabetic rats; insulin therapy increased TGF-beta1 expression inside the callus cells of diabetic rats and improved the biomechanical characteristics of the callus. PMID: 26125699
  86. TSP1-mediated TGFbeta1/Smads pathway activation plays an important role in marcovascular remodeling in T2DM in rat. Valsartan can block the pathway and ameliorate vascular fibrosis. PMID: 25884585
  87. The renoprotective and antifibrosis effects of beta2GPI and reduced beta2GPI on diabetic nephropathy were closely associated with suppressing the activation of the TGF-beta1-p38 MAPK pathway. PMID: 26045739
  88. blocking EGFR can attenuate development of HN via suppression of TGF-beta1 signaling and inflammation and promotion of the molecular processes that reduce uric acid accumulation in the body PMID: 25788532
  89. Results revealed the role of TGFb1 on Wallerian degeneration and provided the basis for the molecular mechanisms of TGFb1-regulated nerve degeneration and/or regeneration PMID: 25451291
  90. DHT suppresses the PGE2 and TGF-beta induced IGF-I gene promoter and differentiates other aspects of TGF-beta activity in osteoblasts PMID: 26187065
  91. KLF4 mediates the link between TGF-beta1-induced gene transcription activation and H3 acetylation during VSMC differentiation. PMID: 26082460
  92. the study suggested that the collagen/SWNT-COOHs nanocomposite films with TGF-beta1 were expected to be useful scaffolds in cartilage tissue engineering. PMID: 26373045
  93. both Smad3 and SREBP-1a activation cooperatively regulate TGFbeta transcriptional responses. PMID: 25348957
  94. demonstrates that OPCs increase BBB tightness by upregulating tight junction proteins via TGF-beta signaling PMID: 25078775
  95. Rats with TNBS-induced chronic colitis exhibited colon fibrosis associated with higher TGF-beta signaling. PMID: 25561788
  96. Pellino1 silencing prevented the binding of NF-kappaB and AP-1 to the promoter region of transforming growth factor-beta1 (TGF-beta1) thus dampening TGF-beta1 transactivation. PMID: 25446187
  97. TGF-beta-induced PRMT1 expression participates in fibroblast proliferation and chronic airway inflammation in ovalbumin-induced asthma. PMID: 26026059
  98. spleen seemed to exhibit an inhibitory effect on liver regeneration by upregulating TGF-beta1 and its receptor TGF-beta-RII in the liver PMID: 25862490
  99. This study showe that TGF-beta and TNF-alpha antagonize the effect of each other on the expression and release of prolactin in a cell line and in obese and diabetic rats. PMID: 25715833
  100. MMP-14 induces an elevation in the levels of TGF-beta1/Smad signaling proteins in injured arteries PMID: 25031710

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Tissue Specificity Abundant in the bone matrix.
Database Links

KEGG: rno:59086

STRING: 10116.ENSRNOP00000028051

UniGene: Rn.40136

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