CARS Recombinant Monoclonal Antibody

Code CSB-RA158403A0HU
Size US$210
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  • Western Blot
    Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate
    All lanes: CARS antibody at 1:500
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 86, 83, 95 kDa
    Observed band size: 95 kDa
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Product Details

Uniprot No.
Target Names
CARS
Alternative Names
Cysteine--tRNA ligase, cytoplasmic (EC 6.1.1.16) (Cysteinyl-tRNA synthetase) (CysRS), CARS
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human CARS
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
8B11
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

To generate the CARS recombinant monoclonal antibody, CUSABIO first obtained the CARS monoclonal antibody. The immunogen used to produce the CARS monoclonal antibody is a synthesized peptide from the human CARS protein. The gene encoding the MAS1L monoclonal antibody is cloned and expressed, and the resulting product is purified using affinity chromatography to ensure high purity. The CARS recombinant monoclonal antibody has been extensively validated for its specificity and quality, and it can recognize and bind specifically to the human CARS protein. This antibody has been tested and proven to work well in ELISA and WB applications.

CARS is an enzyme that belongs to the class of ligases and specifically aminoacyl-tRNA synthetases. Its main function is to catalyze the attachment of cysteine to its corresponding tRNA molecule during protein synthesis. CARS plays an essential role in maintaining the accuracy and fidelity of protein synthesis by ensuring the correct amino acid is incorporated into the growing polypeptide chain. In addition to its primary function in protein synthesis, CARS has also been implicated in other cellular processes such as the regulation of cell growth and proliferation, signal transduction, and stress response.

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Target Background

Function
Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).
Gene References into Functions
  1. Four single nucleotide polymorphisms (SNPs) of CARS were significantly associated with gastric cancer risk in both the discovery stage and the validation stage PMID: 28409418
  2. The risk (G and C) and non-risk (C and T) allele frequencies of the SNPs at the rs1888747 and rs739401 loci of FRMD3 and CARS, respectively, did not differ significantly between the diabetics with (case) and without (control) nephropathy (P > 0.05). PMID: 26909942
  3. Loss of CARS, the cysteinyl-tRNA synthetase, suppresses ferroptosis induced by erastin, which inhibits the cystine-glutamate antiporter known as system xc(-). PMID: 26184909
  4. CARS is a novel fusion partner of anaplastic lymphoma kinase in anaplastic large-cell lymphoma and inflammatory myofibroblastic tumor cases. PMID: 12112524
  5. Specificity of anticodon recognition by human CysRS is higher than that of its bacterial counterparts. PMID: 17303165

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Involvement in disease
A chromosomal aberration involving CARS is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with ALK.
Subcellular Location
Cytoplasm.
Protein Families
Class-I aminoacyl-tRNA synthetase family
Database Links

HGNC: 1493

OMIM: 123859

KEGG: hsa:833

STRING: 9606.ENSP00000369897

UniGene: Hs.274873

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