TSPO Antibody

Datasheet
Code CSB-RA025168A0HU
Size US$350
Uniprot No. P30536
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue
    All lanes: PBR antibody at 1.2μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 19, 11 KDa
    Observed band size: 19 KDa

  • IHC image of CSB-RA025168A0HU diluted at 1:117 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-RA025168A0HU diluted at 1:117 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of PC3 cells with CSB-RA025168A0HU at 1:39, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

  • Immunoprecipitating PTGS2 in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-RA025168A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA025168A0HU (3μg) + Hela whole cell lysate (500μg)
    Lane 3: Hela whole cell lysate (20μg)

  • Overlay histogram showing HepG2 cells stained with CSB-RA025168A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol
Immunogen A synthesized peptide derived from human TSPO
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, IF, FC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000
Relevance Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed:24814875), but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides (PubMed:1847678).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Translocator protein, Mitochondrial benzodiazepine receptor, PKBS, Peripheral-type benzodiazepine receptor, PBR, TSPO, BZRP, MBR
Immunogen Species Human
Research Area Cardiovascular
Gene Names TSPO
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism
Subcellular Location Mitochondrion membrane, Multi-pass membrane protein
Protein Families TspO/BZRP family
Tissue Specificity Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.
Database Links

HGNC: 1158

OMIM: 109610

KEGG: hsa:706

STRING: 9606.ENSP00000328973

UniGene: Hs.202

Pathway Cholesterol metabolism

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