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| Code | CSB-MA000011M0m |
| Size | US$200 |
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| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
6*His monoclonal antibody CSB-MA000011M0m was produced in mouse immunized by using HHHHHH(6x His) synthetic peptide conjugate to KLH as the immunogen. The immunogen, 6x His, a short peptide, consists of six histidine residues (His-His-His-His-His-His). It is specifically designed for the adsorptive purification of recombinant proteins. The relatively small size of His tags makes its integration into expression vectors extremely easy.
This 6*His Monoclonal Antibody was tested in the WB, IHC, ICC and ELISA applications. The non-conjugated IgG2a got purified by protein G and reached up to 95% in purity. It can be used for detection and expression of His-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins and the like. It doesn’t have species restricted. |
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| Target Names | 6*His | ||||
| Alternative Names |
6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
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| Raised in | Mouse | ||||
| Species Reactivity | N/A | ||||
| Immunogen | HHHHHH(6x His) synthetic peptide conjugate to KLH | ||||
| Conjugate | Non-conjugated | ||||
| Clonality | Monoclonal | ||||
| Isotype | IgG2a | ||||
| Clone No. | 3G5H8 | ||||
| Purification Method | >95%, Protein A purified | ||||
| Concentration | It differs from different batches. Please contact us to confirm it. | ||||
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
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| Form | Liquid | ||||
| Tested Applications | ELISA, WB | ||||
| Recommended Dilution |
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| Protocols | ELISA Protocol Western Blotting(WB) Protocol |
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| Troubleshooting and FAQs | Antibody FAQs | ||||
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
Applications : WB
Sample type: Aliquots of the cultures
Review: Validation of anti-SphK2 antibody. Escherichia coli strain BL21 overexpressing His-tagged full length SphK1, His-tagged full length SphK2, and human brain lysate were analyzed by Western blotting using anti-Histidine.
By Anonymous
