Caspase-6 is a protein in humans that is encoded by CASP6 gene. Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
The following CASP6 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CASP6 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004551GA01HU | CASP6 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA004551ESR2HU | CASP6 Antibody |
Human, Mouse | ELISA, WB, IHC |
CSB-PA004551ESR1HU | CASP6 Antibody |
Human | ELISA, WB, IHC |
CSB-PA000006 | Cleaved-CASP6 (D179) Antibody |
Human,Rat | WB, IHC, ELISA |
CSB-PA000084 | CASP6 Antibody |
Human | WB, ELISA |
CSB-PA001232 | CASP6 Antibody |
Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA060289 | Phospho-CASP6 (S257) Antibody |
Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA143409 | CASP6 Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA098112 | CASP6 Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA004551EA01HU | CASP6 Antibody |
Human, Mouse | ELISA, WB, IHC |
CASP6 Antibodies for Mus musculus (Mouse)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA000005 | Cleaved-CASP6 (D162) Antibody |
Mouse,Rat | WB, IHC, ELISA |
CASP6 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP004551HU CSB-EP004551HU CSB-BP004551HU CSB-MP004551HU CSB-EP004551HU-B |
Recombinant Human Caspase-6 (CASP6), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP6 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP004551MO CSB-EP004551MO CSB-BP004551MO CSB-MP004551MO CSB-EP004551MO-B |
Recombinant Mouse Caspase-6 (Casp6) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP6 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP004551RA CSB-EP004551RA CSB-BP004551RA CSB-MP004551RA CSB-EP004551RA-B |
Recombinant Rat Caspase-6 (Casp6) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP6 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP667517BO CSB-EP667517BO CSB-BP667517BO CSB-MP667517BO CSB-EP667517BO-B |
Recombinant Bovine Caspase-6 (CASP6) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
The CASP6 gene encodes caspase-6, an effector caspase that is responsible for apoptosis execution [1]. Caspase-6 is expressed as a dimeric zymogen containing a short prodomain, a large subunit carrying the Cys 163 catalytic cysteine (p20), a small subunit (p10), and an inter‐subunit linker [2]. Although caspase-6 respectively shares partial sequence similarity with caspase-3 and caspase-7, it has several distinctive features. The substrate specificity of caspase-6 is similar to the initiators such as caspase-8 and caspase-9 rather than that of caspase-3 and caspase-7 [11]. The pro-domain of caspase-6 inhibits its self-activation in vivo [3] and in vitro [4]. Caspase-6 is activated by proteolytic processing at the prodomain Asp 23, and both sides of the intersubunit linker Asp 179 (caspase-3 cleavage) and Asp 193 (self-activated cleavage) [5]. Caspase-6 is often activated by caspase-3 rather than initiator caspases during apoptosis [6][7], but it can also be activated in the absence of caspase-3 activity [8][9]. When stimulated by caspase-1, caspase-6 is also associated with the inflammatory pathway [10]. Active caspase-6 is abundant in the neuropathological lesions of Alzheimer's disease [12], Huntington's disease [13], and Parkinson's disease [14]. The physiological outcomes in these neurological disorders are influenced through the cleavage of the neuronal substrates by caspase-6, so it is promising to improve neurodegeneration therapy through caspase-6-targeted therapeutics.
[1] Boatright KM, Salvesen GS Mechanisms of caspase activation [J]. Curr Opin Cell Biol. 2003 Dec; 15(6):725-31.
[2] Nicholson DW Caspase structure, proteolytic substrates, and function during apoptotic cell death [J]. Cell Death Differ, 1999, 6: 1028-1042.
[3] Klaiman G, Champagne N, et al. Self-activation of Caspase-6 in vitro and in vivo: Caspase-6 activation does not induce cell death in HEK293T cells [J]. Biochim Biophys Acta, 2009, 1793:592-601.
[4] Klaiman G, Champagne N, et al. Self-activation of Caspase-6 in vitro and in vivo: Caspase-6 activation does not induce cell death in HEK293T cells [J]. Biochim Biophys Acta 2009, 1793:592-601.
[5] Srinivasula SM, Fernandes‐Alnemri T, et al. The Ced‐3/interleukin 1beta converting enzyme‐like homolog Mch6 and the lamin‐cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32 [J]. J Biol Chem, 1996, 271: 27099-27106.
[6] Slee EA et al. Ordering the cytochrome c‐initiated caspase cascade: hierarchical activation of caspases‐2, ‐3, ‐6, ‐7, ‐8, and ‐10 in a caspase‐9‐dependent manner [J]. J Cell Biol 1999, 144: 281-292.
[7] Simon DJ, et al. A caspase cascade regulating developmental axon degeneration [J]. J Neurosci 2012, 32:17540-17553.
[8] LeBlanc A, Liu H, et al. Caspase-6 role in apoptosis of human neurons, amyloidogenesis, and Alzheimer's disease [J]. J Biol Chem 1999, 274:23426-23436.
[9] Allsopp TE, McLuckie J, et al. Caspase 6 activity initiates caspase 3 activation in cerebellar granule cell apoptosis [J]. Cell Death Differ 2000, 7: 984-993.
[10] Guo H, et al. Caspase-1 activation of caspase-6 in human apoptotic neurons [J]. Cell Death Differ 2006, 13:285-292.
[11] Thornberry NA et al. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis [J]. J Biol Chem 1997, 272: 17907-17911.
[12] Guo H, Albrecht S, et al. Active caspase‐6 and caspase‐6‐cleaved tau in neuropil threads, neuritic plaques, and neurofibrillary tangles of Alzheimer’s disease [J]. Am J Pathol 2004, 165: 523-531.
[13] Aharony I, et al. A Huntingtin-based peptide inhibitor of caspase-6 provides protection from mutant Huntingtin-induced motor and behavioral deficits. [J]. Hum Mol Genet 2015,24:2604-2614.
[14] Giaime E, et al. Loss of function of DJ-1 triggered by Parkinson's disease-associated mutation is due to proteolytic resistance to caspase-6 [J]. Cell Death Differ, 2010, 17:158-169.