LGR4

LGR4 (Leucine-rich repeat-containing G-protein coupled receptor 4), also known as GPR48, is a member of the rhodopsin-like GPCR family that interacts with R-spondin proteins to potentiate Wnt/β-catenin signaling. This receptor-ligand axis critically regulates stem cell maintenance, embryonic development, and tissue regeneration through orchestration of canonical Wnt pathway activation. Emerging studies implicate LGR4 dysfunction in pathological contexts including osteoporosis (via impaired osteoblast differentiation), metabolic disorders (through adipose tissue modulation), and multiple cancers – particularly colorectal and breast malignancies where its overexpression drives tumor proliferation and chemoresistance. Preclinical agents targeting LGR4 include monoclonal antibodies (e.g., FLAG-tagged anti-LGR4 mAbs) and small-molecule inhibitors designed to disrupt R-spondin binding, with several candidates demonstrating antitumor efficacy in xenograft models. Paradoxically, LGR4 agonists are concurrently being explored for bone anabolic therapies, highlighting its context-dependent roles.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

LGR4 Antibody (CSB-PA883618LA01HU)

Validated Data

IHC image of CSB-PA883618LA01HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA883618LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

The following LGR4 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

LGR4 Antibodies

LGR4 Proteins

LGR4 Antibodies

LGR4 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA030195	LGR4 Antibody	Human	WB, IF, ELISA
CSB-PA790514	LGR4 Antibody	Human,Mouse,Rat	ELISA,IHC
CSB-PA883618LA01HU	LGR4 Antibody	Human	ELISA, IHC, IF
CSB-PA883618LB01HU	LGR4 Antibody, HRP conjugated	Human	ELISA
CSB-PA883618LC01HU	LGR4 Antibody, FITC conjugated	Human

LGR4 Antibodies for Danio rerio (Zebrafish) (Brachydanio rerio)

Code	Product Name	Species Reactivity	Application
CSB-PA109051XA01DIL	lgr4 Antibody	Danio rerio (Zebrafish) (Brachydanio rerio)	ELISA, WB (ensure identification of antigen)

LGR4 Proteins

LGR4 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP883618HU CSB-MP883618HU CSB-EP883618HU-B	Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4), partial	Yeast Mammalian cell In Vivo Biotinylation in E.coli
CSB-BP883618HU	Recombinant Human Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4), partial	Baculovirus

LGR4 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP012905MO CSB-EP012905MO CSB-BP012905MO CSB-MP012905MO CSB-EP012905MO-B	Recombinant Mouse Leucine-rich repeat-containing G-protein coupled receptor 4 (Lgr4), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

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LGR4

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