Prelamin-A/C [Cleaved into: Lamin-A/C is a protein in humans that is encoded by LMNA gene. Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation
The following LMNA reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
LMNA Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA013003GA01HU | LMNA Antibody |
Human,Mouse,Rat | ELISA,WB,IF |
CSB-PA000047 | Cleaved-LMNA (D230) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA003130 | LMNA Antibody |
Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA003131 | LMNA Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA009843 | Cleaved-LMNA (N231) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA009844 | Phospho-LMNA (S22) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA009845 | Phospho-LMNA (S392) Antibody |
Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-RA013003A0HU | LMNA Recombinant Monoclonal Antibody |
Human | ELISA, IHC, IF, FC, IP |
CSB-PA013003HA01HU | LMNA Antibody |
Human, Rat | ELISA, WB, IHC, IF |
CSB-PA013003HB01HU | LMNA Antibody, HRP conjugated |
Human | ELISA |
CSB-PA013003HC01HU | LMNA Antibody, FITC conjugated |
Human | |
CSB-PA013003HD01HU | LMNA Antibody, Biotin conjugated |
Human | ELISA |
LMNA Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP013003CH CSB-EP013003CH CSB-BP013003CH CSB-MP013003CH CSB-EP013003CH-B |
Recombinant Chicken Lamin-A (LMNA), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
LMNA Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP013003RA CSB-EP013003RA CSB-BP013003RA CSB-MP013003RA CSB-EP013003RA-B |
Recombinant Rat Prelamin-A/C (Lmna) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
LMNA Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP013003MO CSB-EP013003MO CSB-BP013003MO CSB-MP013003MO CSB-EP013003MO-B |
Recombinant Mouse Prelamin-A/C (Lmna) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
LMNA Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP013003HU CSB-EP013003HU CSB-BP013003HU CSB-MP013003HU CSB-EP013003HU-B |
Recombinant Human Prelamin-A/C (LMNA) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
LMNA Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-YP319246XBE CSB-EP319246XBE CSB-BP319246XBE CSB-MP319246XBE CSB-EP319246XBE-B |
Recombinant Xenopus laevis Lamin-A (lmna), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
LMNA Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-YP673622PI CSB-EP673622PI CSB-BP673622PI CSB-MP673622PI CSB-EP673622PI-B |
Recombinant Pig Prelamin-A/C (LMNA), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
LMNA ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL013003HU | Human lamin A/C (LMNA) ELISA kit |
serum, plasma, tissue homogenates, cell lysates | 3.9 pg/mL |
The human LMNA gene encodes nuclear lamins A and lamins C [1], collectively called A-type lamins, major protein constituents of the mammalian nuclear lamina, a complex structure that acts as a scaffold for protein complexes that regulate nuclear structure and functions [2]. Prelamin A and lamin C are identical through their first 566 amino acids, but then their sequences diverge [1]. Lamins A and C are expressed at very low levels early in embryonic development but are expressed at high levels in most differentiated cells [3-5]. Pre–lamin A undergoes a series of posttranslational modifications that culminate with the cleavage of its farnesylated 15 C-terminal residues to generate mature lamin A [6]. Lamin C does not have a CAAX motif and is never farnesylated in the first place [1]. Besides the well-established role in maintaining the mechanical stability of the nucleus, increasing evidence has shown that A-type lamins and associated nuclear envelope (NE) proteins are scaffolds for proteins that regulate DNA synthesis, responses to DNA damage, chromatin organization, gene transcription, cell cycle progression, cell differentiation, and cell migration [6][7]. Nearly all known LMNA-associated human laminopathies are caused by single "missense" mutations in A-type lamin proteins [8].
[1] Lin F, Worman HJ. Structural organization of the human gene encoding nuclear lamin A and nuclear lamin C [J]. J Biol Chem. 1993 Aug 5; 268(22):16321-6.
[2] Vicente Andrés, José M. González. Role of A-type lamins in signaling, transcription, and chromatin organization [J]. J Cell Biol (2009) 187 (7): 945-957.
[3] Eckersley-Maslin MA, Bergmann JH, et al. Lamin A/C is expressed in pluripotent mouse embryonic stem cells [J]. Nucleus 2013, 4:53-60.
[4] Constantinescu D, Gray HL, et al. Lamin A/C expression is a marker of mouse and human embryonic stem cell differentiation [J]. Stem Cells 2006, 24:177-185.
[5] Rober RA, Weber K, et al. Differential timing of nuclear lamin A/C expression in the various organs of the mouse embryo and the young animal: a developmental study [J]. Development 1989, 105, 365-378.
[6] Broers J.L., Ramaekers F.C., et al. Nuclear lamins: laminopathies and their role in premature ageing [J]. Physiol. Rev. 2006, 86:967-1008.
[7] Verstraeten V.L., Broers J.L., et al. The nuclear envelope, a key structure in cellular integrity and gene expression [J]. Curr. Med. Chem. 2007, 14:1231-1248.
[8] Worman HJ, Fong LG, et al. Laminopathies and the long strange trip from basic cell biology to therapy [J]. J Clin Invest 2009, 119, 1825-1836.