NLK

The following NLK reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

NLK Antibodies

NLK Antibodies for Homo sapiens (Human)

NLK Proteins

NLK Proteins for Mus musculus (Mouse)

NLK Proteins for Rattus norvegicus (Rat)

NLK Proteins for Canis lupus familiaris (Dog) (Canis familiaris)

NLK Proteins for Bos taurus (Bovine)

NLK Proteins for Homo sapiens (Human)

NLK Background

Nemo-like kinase (NLK) is a protein in humans that is encoded by NLK gene [1][2]. NLK is an evolutionary conserved serine/threonine-protein kinase implicated in development, proliferation and apoptosis regulation. As a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Whereas, as a negative regulator of the canonical Wnt/beta-catenin signaling pathway, it binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Ruijie Liu et al. identified NLK as a gene product induced in the hearts of mice subjected to pressure overload or myocardial infarction injury, suggesting a potential regulatory role with pathological stimulation to this organ [3]<、sup>.

[1] Brott BK, Pinsky BA, et al. Nlk is a murine protein kinase related to Erk/MAP kinases and localized in the nucleus [J]. Proc Natl Acad Sci U S A. 1998, 95 (3): 963–8.
[2] Kehrer-Sawatzki H, Moschgath E, et al. Characterization of the Fugu rubripes NLK and FN5 genes flanking the NF1 (Neurofibromatosis type 1) gene in the 5' direction and mapping of the human counterparts [J]. Gene. 2000,251 (1): 63–71.
[3] Ruijie Liu, Hadi Khalil, et al. Nemo-Like Kinase (NLK) Is a Pathological Signaling Effector in the Mouse Heart [J]. PLoS One. 2016; 11(10): e0164897.

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