PDCD1, programmed cell death protein 1, also known as PD1, CD279, and hSLE1, is encoded by the human PDCD1 gene, which is located on chromosome 2 at position q37.3.
The PDCD1 protein is an important immunosuppressive receptor, a member of the immunoglobulin superfamily, and is expressed mainly on activated T and B cells. Its mechanism of action involves binding to the ligands PD-L1 and PD-L2, which initiates programmed death of T cells, thereby inhibiting T cell activation and proliferation. PDCD1 plays an important role in maintaining peripheral T and B cell tolerance, and its enhanced signaling downregulates the response of activated T and B cells in peripheral tissues.
Abnormal expression of PDCD1 is associated with a variety of autoimmune diseases, infectious diseases and tumors, and is an important target for immunotherapy.
Recombinant Human Programmed cell death protein 1 (PDCD1), partial (Active) (CSB-MP619964HU1)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Anti-PD-1 recombinant antibody, the EC50 of human PD-1 protein is 6.087-7.854 ng/ml.
Activity
②Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Nivolumab, the EC50 of human PD-1 protein is 9.713-12.39 ng/ml.
PDCD1 Recombinant Monoclonal Antibody (CSB-RA240597A0HU)
Validated Data
Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: PD1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32 KDa
Observed band size: 32 kDa
IHC image of CSB-RA240597A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
The following PDCD1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
PDCD1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA017664GA01HU | PDCD1 Antibody | Human | ELISA,WB,IHC |
CSB-PA483633 | PDCD1 Antibody | Human | ELISA,IHC |
CSB-PA619964LD01HU | PDCD1 Antibody, Biotin conjugated | Human | ELISA |
CSB-PA619964LC01HU | PDCD1 Antibody, FITC conjugated | Human | |
CSB-PA619964LB01HU | PDCD1 Antibody, HRP conjugated | Human | ELISA |
CSB-PA619964LA01HU | PDCD1 Antibody | Human, Mouse | ELISA, WB, IHC, IF |
CSB-RA240597A0HU | PDCD1 Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC |
CSB-MA619964A0m | PD-1 Monoclonal Antibody | Human | ELISA, WB |
CSB-MA619964A1m | PD-1 Monoclonal Antibody | Human | ELISA, WB |
CSB-RA619964MA1HU | PD-1 Recombinant Monoclonal Antibody | Human | ELISA |
PDCD1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP619964HU CSB-EP619964HU-B |
Recombinant Human Programmed cell death protein 1 (PDCD1), partial | Yeast In Vivo Biotinylation in E.coli |
CSB-EP619964HU | Recombinant Human Programmed cell death protein 1 (PDCD1), partial | E.coli |
CSB-BP619964HU | Recombinant Human Programmed cell death protein 1 (PDCD1), partial | Baculovirus |
CSB-MP619964HU1 | Recombinant Human Programmed cell death protein 1 (PDCD1), partial (Active) | Mammalian cell |
PDCD1 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E13643h | Human Programmed Death 1(PD-1)ELISA KIT | serum, plasma, tissue homogenates, cell lysates | 3.9 pg/mL |
PDCD1 ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E13586m | Mouse Programmed Death 1(PD-1)ELISA Kit | serum, plasma, tissue homogenates, cell lysates | 0.078 ng/mL |