Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Anti-PD-1 recombinant antibody, the EC50 of human PD-1 protein is 6.087-7.854 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Nivolumab, the EC50 of human PD-1 protein is 9.713-12.39 ng/ml.
Alternative Names
(Protein PD-1) (hPD-1) (CD279)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
25-167aa
Target Protein Sequence
LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ
Tag Info
C-terminal 6xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
CUSABIO used a DNA fragment (encoding amino acid 25-167 of the human PDCD1) with a C-terminal 6xHis-tag to generate the recombinant human PDCD1 protein in mammalian cells. This PDCD1 protein was subjected to SDS-PAGE under reducing conditions and presented a molecular mass band of about 30 kDa on the gel. Its purity is greater than 95%. The endotoxin content is less than 1.0 EU/ug as determined by the LAL method. It has been identified as an active protein through its binding ability with the anti-PDCD1 antibody or Nivolumab in the functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Anti-PD-1 recombinant antibody with the EC50 of 6.087-7.854 ng/ml. Immobilized PD-1 at 2 μg/ml can bind Nivolumab with the EC50 of 9.713-12.39 ng/ml. And it is in stock now.
PDCD1, also called PD1 or CD279, is an immunoinhibitory receptor expressed by all T cells during activation. It plays a critical role in balancing protective immunity and immunopathology, homeostasis, and tolerance. PD-1/PD-L1 pathway is responsible for cancer immune evasion and has become the target for cancer treatment.