PEAR1, a transmembrane receptor predominantly found on platelets and endothelial cells, is encoded by a gene with 23 exons and 22 introns. This receptor is instrumental in platelet activation and aggregation, contributing to the progression of various vascular diseases. PEAR1's mechanism includes binding to platelet surface ligands to transmit activation signals and modulate endothelial cell function, thereby facilitating interactions between platelets and endothelial cells. It is crucial for vascular homeostasis, injury repair, and endothelial function regulation. The genetic variations in PEAR1 are closely associated with platelet aggregation and vascular function, highlighting its potential as a therapeutic target for cardiovascular diseases.
PEAR1 Antibody (CSB-PA017765LA01HU)
Validated Data
Western Blot
Positive WB detected in: Rat liver tissue, Mouse kidney tissue
All lanes: PEAR1 antibody at 5.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 111 kDa
Observed band size: 111 kDa
IHC image of CSB-PA017765LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with CSB-PA017765LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The following PEAR1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
PEAR1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA017765LA01HU | PEAR1 Antibody | Human, Rat, Mouse | ELISA, WB, IHC, IF |
CSB-PA017765LB01HU | PEAR1 Antibody, HRP conjugated | Human | ELISA |
CSB-PA017765LC01HU | PEAR1 Antibody, FITC conjugated | Human | |
CSB-PA017765LD01HU | PEAR1 Antibody, Biotin conjugated | Human | ELISA |
PEAR1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP017765HU CSB-EP017765HU CSB-BP017765HU CSB-MP017765HU CSB-EP017765HU-B |
Recombinant Human Platelet endothelial aggregation receptor 1 (PEAR1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |