Ribonuclease H1 (encoded by RNASEH1) is an Mg²+-dependent endonuclease that cleaves the RNA strand of RNA-DNA hybrids. Common aliases include RNase H1 and HNRNPK-associated ribonuclease. It plays key roles in DNA replication (removing RNA primers from Okazaki fragments) and repair (resolving R-loops to prevent genome instability).
Dysregulation of RNASEH1 links to Aicardi-Goutières syndrome, a rare neuroinflammatory disorder caused by loss-of-function mutations. Overexpression is seen in cancers like breast and lung, supporting tumor proliferation via replication stress tolerance. Preclinical research explores small-molecule inhibitors of RNase H1 as potential anticancer agents, often combined with nucleoside analogs to enhance efficacy.
Recombinant Human Ribonuclease H1 (RNASEH1) (D210N) (CSB-EP019801HU(M))
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
RNASEH1 Antibody (CSB-PA019801LA01HU)
Validated Data
Western Blot
Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate
All lanes: RNASEH1 antibody at 3.59µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
IHC image of CSB-PA019801LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA019801LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
The following RNASEH1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
RNASEH1 Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA019801GA01HU | RNASEH1 Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA019801LA01HU | RNASEH1 Antibody | Human | ELISA, WB, IHC |
| CSB-PA019801LB01HU | RNASEH1 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA019801LC01HU | RNASEH1 Antibody, FITC conjugated | Human | |
| CSB-PA019801LD01HU | RNASEH1 Antibody, Biotin conjugated | Human | ELISA |
RNASEH1 Proteins for Mus musculus (Mouse)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP019801MO CSB-EP019801MO CSB-BP019801MO CSB-MP019801MO CSB-EP019801MO-B |
Recombinant Mouse Ribonuclease H1 (Rnaseh1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RNASEH1 Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP019801HU CSB-BP019801HU CSB-MP019801HU CSB-EP019801HU-B |
Recombinant Human Ribonuclease H1 (RNASEH1) | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP019801HU | Recombinant Human Ribonuclease H1 (RNASEH1) | E.coli |
| CSB-EP019801HUc7 | Recombinant Human Ribonuclease H1 (RNASEH1) | E.coli |
| CSB-EP019801HU(M) | Recombinant Human Ribonuclease H1 (RNASEH1) (D210N) | E.coli |