TMEM67

Western Blot
Positive WB detected in: K562 whole cell lysate(30µg), A431 whole cell lysate(30µg), Hela whole cell lysate(30µg), U-251MG whole cell lysate(30µg), HEK293 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), Rat brain tissue lysate(30µg)
All lanes: Meckelin polymerase beta antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 112 kDa
Observed band size: 112, 122 kDa
Exposure time：1min

Immunofluorescence staining of U-251MG cell with CSB-RA780441A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing 786-O cells stained with CSB-RA780441A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA689243LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA689243LA01HU at dilution of 1:100

Immunofluorescent analysis of HepG2 cells using CSB-PA689243LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)