Dog Transforming growth factor β1,TGF-β1 ELISA Kit

Instructions
Code CSB-E04796c
Size 96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name transforming growth factor, beta 1
Alternative Names TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
Abbreviation TGFB1
Uniprot No. P54831
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma
Detection Range 0.781 ng/ml - 50 ng/ml
Sensitivity 0.747 ng/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 81-89  
1:2 Average % 99  
Range % 94-103  
1:4 Average % 87  
Range % 84-91  
1:8 Average % 100  
Range % 94-106  
Recovery
The recovery of dog TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-97  
EDTA plasma (n=4) 97 94-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
50 2.408 2.441 2.425 2.285  
25 2.282 2.100 2.191 2.051  
12.5 1.831 1.898 1.865 1.725  
6.25 1.372 1.398 1.385 1.245  
3.125 0.718 0.732 0.725 0.585  
1.563 0.402 0.412 0.407 0.267  
0.781 0.251 0.250 0.251 0.111  
0 0.141 0.139 0.140    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (By similarity). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (By similarity). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (By similarity).
Gene References into Functions
  1. Results suggested that CD61 (integrin-beta3) could induce canine adipose-derived mesenchymal stem cells (cADMSCs) to differentiate into primordial germ cell (PGC)-like cells by relying on the activation of transforming growth factor beta (TGF-beta) pathway. PMID: 28256590
  2. obstructive sleep apnea can accelerate the progression of pulmonary remodeling through TGF-beta/miR-185/CoLA1 signaling PMID: 27542203
  3. TGFbeta1 signaling through the canonical SMAD pathway is increased in myxomatous mitral valves, but does not apparently mediate interstitial cell apoptosis in canine MMVD. PMID: 23816827
  4. IL-6 markedly decreased the nuclear concentration of a TGF-beta signaling transducer, Smad 2/3. PMID: 23579028
  5. celastrol downregulates Snail expression, thereby inhibiting TGF-beta1-induced EMT in MDCK and A549 cells PMID: 23850675
  6. Ostectomy healing with or without platelet-rich plasma as indicated by radiography and expression of PDGF and TGF-beta expression are reported. PMID: 23111655
  7. This study tested the hypothesis that cyclic strain and TGFbeta1 interact to modify mitral valve interstitial cell phenotype in 3D culture. PMID: 22386586
  8. PAK2 negatively modulate TGF-beta signaling by attenuating the receptor-Smad interaction and thus Smad activation PMID: 22393057
  9. This study showed a lack of an effect of recombinant inteferon-gamma on transforming growth factor-beta (TGF-beta) expression in keratinocytes. PMID: 20860556
  10. Wnt-5a is involved during oncogenic H-Ras/TGF-beta-mediated epithelial-mesenchymal transition PMID: 20511395
  11. These results show basolateral domain specificity in localization of the TGFbeta receptor signaling apparatus. PMID: 20432436
  12. These findings suggest that polyunsaturated fatty acids are able to influence proliferation of peripheral blood mononuclear cells in healthy and atopic dogs but do not seem to influence cytokine transcription. PMID: 20187917
  13. Transforming growth factor-beta 1 secreted by canine transmissible venereal tumor cells suppresses lymphokine-activated killer cytotoxicity. PMID: 14734728
  14. role played by TGF-beta in mediating the cyclosporine-induced activation of ERK1/2 and the resulting increase in transepithelial electric resistance PMID: 17460148
  15. TGFbeta1 might be associated with pulmonary vein stenosis after radiofrequency ablation in chronic atrial fibrillation of dogs PMID: 17972164

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Database Links

KEGG: cfa:403998

STRING: 9615.ENSCAFP00000007462

UniGene: Cfa.3509

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