Human Coagulation factor XI(F11) ELISA kit

Instructions
Code CSB-EL007916HU
Size 96T,5×96T,10×96T

Product Details

Target Name coagulation factor XI
Alternative Names FXI, MGC141891, OTTHUMP00000197033|plasma thromboplastin antecedent
Abbreviation F11
Uniprot No. P03951
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Blood Coagulation
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human F11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 85
Range % 82-88
1:2000 Average % 110
Range % 107-113
1:4000 Average % 91
Range % 85-97
1:8000 Average % 92
Range % 87-95
Recovery
The recovery of human F11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 98-106
EDTA plasma (n=4) 86 82-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
5000 2.704 2.681 2.693 2.617
2500 2.092 2.138 2.115 2.039
1250 1.387 1.405 1.396 1.320
625 0.845 0.868 0.857 0.781
312 0.503 0.538 0.521 0.445
156 0.301 0.296 0.299 0.223
78 0.176 0.186 0.181 0.105
0 0.077 0.074 0.076  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
Involvement in disease Factor XI deficiency (FA11D)
Subcellular Location Secreted
Protein Families Peptidase S1 family, Plasma kallikrein subfamily
Tissue Specificity Isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells.
Database Links

HGNC: 3529

OMIM: 264900

KEGG: hsa:2160

STRING: 9606.ENSP00000384957

UniGene: Hs.1430

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