Mouse Transforming Growth factor β1,TGF-β1 ELISA kit

Instructions
Code CSB-E04726m
Size 96T,5×96T,10×96T

Product Details

Target Name transforming growth factor, beta 1
Alternative Names CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 protein|latency-associated peptide
Abbreviation TGFB1
Uniprot No. P04202
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.2 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 99  
Range % 94-104  
1:2 Average % 89  
Range % 83-97  
1:4 Average % 92  
Range % 87-97  
1:8 Average % 92  
Range % 87-96  
Recovery
The recovery of mouse TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-99  
EDTA plasma (n=4) 96 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
50 2.654 2.636 2.645 2.515  
25 2.318 2.455 2.387 2.257  
12.5 1.756 1.886 1.821 1.691  
6.25 0.997 1.001 0.999 0.869  
3.12 0.542 0.512 0.527 0.397  
1.56 0.301 0.309 0.305 0.175  
0.78 0.228 0.238 0.233 0.103  
0 0.129 0.131 0.130    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Target Data

Function Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Database Links

KEGG: mmu:21803

STRING: 10090.ENSMUSP00000002678

UniGene: Mm.248380

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