Recombinant Human Interleukin-2 protein(IL2) (Active)

In Stock
Code CSB-AP001691HU
Size US$873Purchase it in Cusabio online store
(only available for customers from the US)
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0x107 IU/mg.
Target Names IL2
Uniprot No. P60568
Research Area Immunology
Alternative Names Aldesleukin; IL 2; IL-2; IL2; IL2_HUMAN; Interleukin 2; Interleukin-2; interleukin2; Involved in regulation of T cell clonal expansion; Lymphokine; OTTHUMP00000164090; POIL2; T Cell Growth Factor; T-cell growth factor; TCGF
Species Homo sapiens (Human)
Source E.coli
Expression Region 21-153aa
Mol. Weight 15.4 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer 0.2 μm filtered 20 mM PB, pH 3.5 ,lyophilized
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Gene References into Functions
  1. the co-culture with human T lymphocytes induce the in vitro maturation of human intestinal organoids, and identify STAT3-activating interleukin-2 (IL-2) as the major factor inducing maturation. PMID: 30072687
  2. This study emphasized on the bone marrow and blood plasma levels of IL-2 in aplastic anaemia and their relationship with disease severity. The results indicate towards the fact that IL-2 may have an important association with the marrow failure of AAA patients and thus can help in disease development. Further study is necessary for better understanding. PMID: 30139310
  3. The present study demonstrated no association between IL-2 (T-330G), IL-16 (T-295C), and IL-17 (A-7383G) genotypes and CP in an Iranian population. PMID: 29400002
  4. In contrast with some reports involving the correlation between polymorphisms of the TGF-beta1 and IL-2 genes and inhibitor development in the world, no statistically significant differences in analysis of the alleles and genotypes for TGF-beta and IL-2 genes were found between the inhibitor and non-inhibitor Iranian patients PMID: 29993342
  5. The current study highlights the possible involvement of the IL-2-330T/G Single Nucleotide Polymorphism in susceptibility to B-Cell Non-Hodgkin Lymphoma . Moreover, IL-10-1082A/G is not a molecular susceptibility marker for B-Cell Non-Hodgkin Lymphoma in Egyptians. PMID: 28713071
  6. interleukin-2 (IL-2) is a non-pancreatic autoimmune target in type 1 diabetes PMID: 27708334
  7. The IL-2 AC genotype and C allele of IL-2 (-330A>C) gene polymorphisms could be potential protective factors and might reduce the risk of oral cancer in Indian population. PMID: 29664031
  8. Data show that GIF (ORF117 ) serves as a competitive decoy receptor by leveraging binding hotspots underlying the cognate receptor interactions of granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin-2 (IL-2). PMID: 27819269
  9. CD4(+) T cells showed the greatest increase (threefold) in ORMDL3 expression in individuals carrying the asthma-risk alleles, where ORMDL3 negatively regulated interleukin-2 production. The asthma-risk variants rs4065275 and rs12936231 switched CTCF-binding sites in the 17q21 locus. PMID: 27848966
  10. The main mechanism of elimination of mesenchymal stromal cells is cytotoxicity of NK cells that depended on IL-2 production. PMID: 29313232
  11. Uremia patients receiving maintenance hemodialysis with hospitalacquired infection had increased serum inflammatory factors and high throughput hemodialysis significantly decreased CRP, IL2 and TNFalpha levels in the serum, suggesting that high throughput hemodialysis may be beneficial for the prevention of the infections in uremia patients. PMID: 29257244
  12. The simultaneous delivery of multiple proinflammatory payloads to the cancer site conferred protective immunity against subsequent tumor challenges. A fully human homolog of IL2-F8-TNF(mut), which retained selectivity similar to its murine counterpart when tested on human material, may open new clinical applications for the immunotherapy of cancer. PMID: 28716814
  13. Study found that in SLE patients lymphocyte production of IL-2 did not decrease when compared with that of normal subjects. PMID: 29911835
  14. Peripheral blood Tregs failed to effectively utilize IL-2 and had relatively little STAT5 phosphorylation in active ankylosing spondylitis. PMID: 27901054
  15. correction for individual cytokine expression levels revealed qualitative differences of cytokine profiles characterized by significantly increased TNFalpha and decreased IL-2-expressing T-cell proportions in long-term type-1-diabetes patients. PMID: 28377612
  16. CD45 is a regulator of IL-2 synergy in the NKG2D-mediated activation of immature human NK cells PMID: 28655861
  17. genetic polymorphism is associated with increased susceptibility to chronic spontaneous urticaria in Iran PMID: 28159384
  18. PLX4032, a selective BRAF-i, has no inhibitory effect either on NK cell proliferation in response to cytokines IL-2 or IL-15 PMID: 27563819
  19. Results suggest that IL-6 and IL-2 were influenced non-specifically by the presence of a mental disorder and by demographic variables of gender, ethnicity and BMI. Implications of these findings are discussed, as well as possible long-term impact of the identified interleukin differences on immunologic, inflammatory, neuropsychiatric and other systems. PMID: 28486207
  20. possible associations between the IL-2 polymorphisms +114 T>G (rs2069763) and -330 T>G (rs2069762) and the development of gastric cancer; these associations were then correlated with the presence of H. pylori; results show that, among patients with H. pylori infection, the -330 GG and +114 TT genotypes are significantly associated with a high risk of developing gastric cancer, as is the -330G/+114T haplotype PMID: 28458166
  21. Overrepresentation of certain alleles, genotypes and haplotypes in IL-2 gene in febrile seizure patients could predispose individuals to this disease PMID: 28843235
  22. Data reported that let-7i upregulates IL-2 expression by targeting the promoter TATA-box region, which functions as a positive regulator. In HIV-1 infection, the expression of let-7i in CD4(+) T cells is decreased by attenuating its promoter activity. The reduced let-7i miRNA expression led to a decline in IL-2 levels which contributes to CD4(+) T cells death. PMID: 27145859
  23. IL-2 and IL-6 work together to enhance influenza-specific CD8 T cell generation responding to live influenza virus in aged mice and humans PMID: 27322555
  24. the aim of the current review article is to determine the roles of IL-2 IL-2/IL-2R interaction in polyoma BK virus reactivation and PBK associated nephropathy complications. PMID: 27718431
  25. Modulation of IL-2, IL-4, IFN-gamma and/or TNF-alpha levels, or inhibitors of Erk1/2 or S6K1 may be a new approach to prevent BAFF-induced aggressive B-cell malignancies. PMID: 27235588
  26. Our results show that the frequency of Tregs is altered in a large cohort of long-term T1D patients, a profound decrease in CD25 expression and altered IL-2 signaling are typical features of Tregs populations in long-term diabetic patients and their relatives. PMID: 27560779
  27. a role for circulating IL-2 in liver dysfunction and propose that a combined assessment of AST/ALT in conjunction with IL-2 at the early stages of symptomatic DENV infection may be useful to predict the severe forms of dengue. PMID: 27155816
  28. Lymphocytes incubated in the presence of IL-2 lose the capacity for chemotaxis but acquire antitumor activity PMID: 28429264
  29. the central biological role of the novel IL-2-R/Lck/PLCgamma/PKCtheta;/alphaPIX/Rac1/PYGM signalling pathway is directly related to the control of fundamental cellular processes such as T cell migration and proliferation. PMID: 27519475
  30. F42K can circumvent the expansion and negative immunoregulatory effects of highly suppressive ICOS+ Tregs, while promoting NK cell expansion and function. F42K also induces a unique gene expression profile and does not activate many IL2-induced genes, although it has the capacity to activate NK cells and NK cell-associated activation genes, costimulatory molecules, and NK-mediated cytolytic function PMID: 27697858
  31. Chimeric fusion protein of interleukin 2 (IL2) and its receptor interleukin 2 receptor, beta protein (IL2Rbeta) enhances antitumor activity of natural killer NK92 cells. PMID: 28916655
  32. Il2 was not a useful discriminative markers for active tuberculosis among pulmonary tuberculosis suspects. PMID: 27450011
  33. IL-2 and IL-10 could work synergistically to improve the survival, proliferation, and cytotoxicity of activated CD8(+) T cells, an effect suppressible by CD4(+)CD25(+) Treg cells PMID: 28274688
  34. results show that IL-23 accounts for the main aspects of human and murine lupus including the expansion of double negative T cells, decreased IL-2, and increased IL-17 production PMID: 28646040
  35. Report efficient retroviral vector transduction of primary human natural killer cells that were stimulated by a combination of IL-2 and engineered K562 cells expressing membrane-bound IL-21 for cancer immunotherapy. PMID: 28802832
  36. upon ligation of the T-cell antigen receptor (TCR), the TCR associates with and transactivates CXCR4 via phosphorylation of S339-CXCR4 in order to activate a PREX1-Rac1-signaling pathway that stabilizes interleukin-2(IL-2), IL-4, and IL-10 messenger RNA (mRNA) transcripts. PMID: 28694325
  37. IL2 induces disruption of adherens junctions, concomitant with cytoskeletal reorganization, ultimately leading to increased endothelial cell permeability. PMID: 27601468
  38. findings extend understanding of the differential modes of action between IL-2 and IL-15, and highlight how, although sharing the CD122/CD132 receptor, they elicit such different immune actions PMID: 28507024
  39. DNA-PKcs is a potent regulator of IL-2 production in T lymphocytes. PMID: 28750002
  40. Yeast-expressed human IL-2 fusion toxin effectively targetedCD25(+) cutaneous T cell lymphoma. PMID: 28551309
  41. study provides evidence that membrane-coated microvesicles released by apoptotic neutrophils suppress a subset of resting Th cells by downregulating IL-2 and IL-2R expression PMID: 28295230
  42. In T1D, low IL-2 responsiveness was most pronounced in memory T effector cells. Reduced IL-2 responses in memory T effector cells were not rescued by resting, remained lower after activation and proliferation, and were absent in type 2 diabetes. PMID: 28645874
  43. the elevated level of IL-2+ and IL-21+ T cells and a positive correlation between IL-21+ cells with clinical activity index in ulcerative colitis patients may contribute to the pathogenesis of disease. PMID: 28685527
  44. analysis of the contribution of IL2Rgamma to the dynamic formation of IL2-IL2R complexes PMID: 27195783
  45. lack of local IL-2 enhances regulatory T-cell susceptibility to Fas-mediated apoptosis induced by epithelial cells PMID: 26928938
  46. The IL-2 rs2069762 G allele appeared to be a protective mutation against aplastic anemia, but no significant differences were found in other four IL-2 and Il-8 SNPs studied. PMID: 28268223
  47. Pristimerin inhibits IL-2 induced T cell activation and generation of lymphokine-activated killer cells by disrupting multiple cell signaling pathways induced by IL-2. PMID: 28471123
  48. IL-2 or induced killer cells combination therapy was efficacious in treating NSCLC and improved overall survival. Further analysis of trials having adequate information and data need to be done to confirm these findings. PMID: 27748271
  49. Treatment of memory CD4 T cells with the concentration of kynurenine found in plasma inhibited IL-2 signaling through the production of reactive oxygen species. PMID: 27356894
  50. Ochratoxin A mediates MAPK activation, modulates IL-2 and TNF-alpha mRNA expression and induces apoptosis by mitochondria-dependent and mitochondria-independent pathways in human CD4 positive T-cell lymphoma cell line. PMID: 27193732
  51. The results of the current study suggest that certain SNPs of IL-2 gene have association with individuals' susceptibility to juvenile idiopathic arthritis. PMID: 27040810
  52. this study shows that IL-2 cytokine gene polymorphisms could affect individual susceptibility to Juvenile systemic lupus erythematosus in Iranian children PMID: 27255473
  53. There is a strong association between IL2/IL21 rs6822844 variant and susceptibility to rheumatoid arthritis (RA) in the Algerian population; this association is independent from the autoantibodies status of RA patients. PMID: 26917059
  54. Production of IL2 in follicular fluid lymphocytes of PCOS patients was significantly higher than that in controls. IL2 concentration in follicular fluid of PCOS patients was significantly higher than that in controls. PMID: 26751822
  55. The polymorphisms in IL-2 and IL-10 genes may contribute to basal-cell carcinoma (BCC) susceptibility and influence the clinical course of BCC in polish population. PMID: 26609634
  56. IL-2 may be involved in the pathogenesis of recurrent oral ulcer. PMID: 26722428
  57. level of expression correlates with pain response in subjects with intervertebral disc disorders PMID: 26440592
  58. After 4 weeks of star fruit juice consumption, a significant reduction in NO, TNF-alpha and IL-23 was found; however, there was no change in IL-2. PMID: 26952371
  59. There was a significant association of IL2 rs6534349 polymorphism with increased asthma risk and high load M. pneumoniae infectionin the GG genotype. IL-2 serum expression levels were significantly higher in M. pneumoniae negative subjects. PMID: 25747600
  60. Triple costimulation via CD80, 4-1BB, and CD83 ligand elicits the long-term growth of Vgamma9Vdelta2 T cells in low levels of IL-2. PMID: 26561569
  61. Single Nucleotide Polymorphisms in interleukin 2 gene involved in the immune response are generally not associated with intraprostatic inflammation in men without a Prostate cancer diagnosis. PMID: 26771888
  62. TIM-3 is a negative regulator of TCR-function by attenuating activation signals mediated by CD3/CD28 co-stimulation. PMID: 26492563
  63. IL-2-330 T/G polymorphism may not be associated with acute rejection risk in renal transplant recipients (Meta-Analysis) PMID: 26293045
  64. Age and CMV serostatus influence the expression of NKp30, NKp46 and DNAM-1 activating receptors on resting and IL-2 activated natural killer cells. PMID: 25991472
  65. Moxifloxacin restored IL-2 expression and T-cell proliferation in the presence of LPS by blocking p38 MAPK PMID: 26498252
  66. The results suggested that the IL-2 +114T/G polymorphism may contribute to increased HBV-related HCC risk through regulating the serum IL-2 levels. PMID: 25173083
  67. Common gamma-chain cytokines IL-2, IL-7, and IL-15 prevent differentiation of naive T cells in vitro and limit activation of primed T cells in the absence of antigenic stimulus, which can contribute to the formation of cytokine imbalance. PMID: 26085358
  68. Data show that metal nanoparticles AgNPs and AuNPs modified PMA/PHA/LPS induced cytokines IL-2, IL-6, and TNF-alpha production in Jurkat and U937 leukemic cells. PMID: 25966134
  69. Data show that JAK/STAT signaling inhibition is potentiated by Bcl-xL (B-cell lymphoma-extra large) blockade in interleukin 2 (IL-2) dependent adult T-cell leukemia cells. PMID: 26396258
  70. Targeting interleukin-2 to the bone marrow stroma for therapy of acute myeloid leukemia relapsing after allogeneic hematopoietic stem cell transplantation PMID: 25672398
  71. High serum levels of interleukin-2, a pro-inflammatory non-vascular cytokine, are associated with the recurrence of atrial fibrillation in patients undergoing catheter ablation. PMID: 25743240
  72. ADP-ribosylation inhibits binding of IL-2 by CD25, IL-2- induced phosphorylation of STAT5, and IL-2-dependent cell proliferation. PMID: 25753532
  73. The result confirm specific steroid 21-hydroxylase-directed reactivity of the peripheral Addison's disease lymphocytes, which display increased synthesis of interleukin-2 and soluble IL2Ra. PMID: 25347332
  74. possible role of an miR-155-PP2Ac loop in regulating IL-2 release PMID: 25253569
  75. IL2RB thr-450 phosphorylation is a positive regulator for receptor-IL-2 complex stability and activation of signaling molecules. PMID: 26152718
  76. Interleukin 2 is downregulated in T cell exhaustion [review] PMID: 25516298
  77. production of IL-2 was significantly decreased in latently HIV-1-infected cells compared with uninfected parent cells PMID: 26086100
  78. Human group3 innate lymphoid cells express DR3 and respond to TL1A with enhanced IL-22 production and IL-2-dependent proliferation. PMID: 26046454
  79. The inflammatory response to human chorionic gonadotropin, leading to dysregulation of Il-2 expression and SOCS activation, may be factors in ovarian hyperstimulation syndrome. PMID: 25424734
  80. IL-2/IL-2R and B7-H3 pathways may be involved in the progression of clear cell renal cell carcinoma PMID: 25089268
  81. there was no association of SNPs in IL2 or IL6 and antipsychotic-induced weight gain PMID: 25560300
  82. Our results identify core mechanisms dictating human tTreg development, with IL-2 and IL-15 defining specific niches required for tTreg lineage stabilization and differentiation, with implications for their therapeutic targeting in autoimmune conditions. PMID: 25481744
  83. Chronic gastroduodenitis is accompanied by the increase of IL-2 and TNF-alpha concentration thus reflecting the activity of apoptotic processes PMID: 26281156
  84. For a complete analysis of our results on the reduction of the cytokines (IL-2, IL-6) content in patients with a stress in anamnesis, that will allow to identify the most effective ways to treat patients with this pathology PMID: 25416216
  85. The unique role of IL2 through maintenance of fitness of regulatory T cells and activation-induced cell death is the elimination of self-reactive T cells to prevent autoimmunity. [review] PMID: 25736261
  86. Interleukins IL-2, IL-6 and IL-10 are promising biomarkers of early-stage melanoma. IL-2 and IL-6 appear to be prognostic biomarkers. PMID: 26026122
  87. This review is a summary and discussion of the features and biological relevance of several enhanced IL-2 formulations. PMID: 25271022
  88. It seems that the rs6822844 IL2-IL21 gene cluster polymorphism is of little importance in allograft function after kidney transplantation. PMID: 25377634
  89. Thymus medullary epithelial cells promote regulatory T-cell generation by stimulating interleukin-2 production via ICOS. PMID: 25210803
  90. Cytokine and chemokine responses to selected early secreted antigenic target-6 and culture filtrate protein-10 peptides in tuberculosis. PMID: 21133811
  91. Single nucleotide polymorphisms in the IL-2 and IL-2R genes are associated with tumor- killing activity of lymphokine-activated killer cells. PMID: 25292096
  92. This review discusses the role of IL-2 in pathogenesis of autoimmune diseases, particularly regarding its positive considerable impact on Treg cell fitness and suppressive function. [review] PMID: 25322151
  93. Data indicate that interleukin 2 receptor alpha (IL2RA) single nucleotide polymorphism (SNPs) and key genes of the IL-2 pathway are also associated with cutaneous leishmaniasis (CL). PMID: 25672756
  94. Were highly methylated at the IL2 locus. PMID: 25001463
  95. intracellular interleukin-2 in CD8+ T-cells is increased after organ rejection in kidney transplant recipients PMID: 24887424
  96. Genetic variation in interleukin-2 gene is associated with breast cancer risk. PMID: 24670917
  97. Human herpesvirus 6B U54 binds the calcineurin (CaN) phosphatase enzyme, causing improper dephosphorylation and nuclear translocation of NFAT (nuclear factor of activated T cells) proteins, resulting in suboptimal IL-2 gene transcription. PMID: 25122797
  98. Genetic polymorphism in IL-2 is not associated with acute rejection in renal transplantation. PMID: 24579958
  99. NK cell activity in children with IDDM after in vitro stimulation by IL2 inhibits autoimmunity by increased IL10 synthesis. PMID: 25577895
  100. Vitamin D3 further suppressed Il-2 production in T-lymphocytes from systemic lupus erythematosus patients. PMID: 23612780

Show More

Hide All

Involvement in disease A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
Subcellular Location Secreted
Protein Families IL-2 family
Database Links

HGNC: 6001

OMIM: 147680

KEGG: hsa:3558

STRING: 9606.ENSP00000226730

UniGene: Hs.89679

Most popular with customers

  Tel: 301-363-4651
  Distributors Worldwide


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1