Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA(CSB-MP011649HU3) at 2 μg/mL can bind Human IL2 , the EC50 is 0.4992 to 0.6269 ng/mL.
Alternative Names
IL-2;T-cell growth factor (TCGF);Aldesleukin
Species
Homo sapiens (Human)
Expression Region
21-153aa
Target Protein Sequence
APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal hFc-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The gene segment coding for the full-length mature human interleukin-2 (IL2) protein (21-153aa) is fused with a hFc-tag gene at the N-terminus and then integrated into a plasmid vector. The recombinant vector is transfected into mammalian cells for protein expression. The recombinant human IL2 protein is collected from the cell lysate. Its purity is over 95% determined by SDS-PAGE. Its endotoxin is less than 1.0 EU/ug measured by the LAL method. Its activity is measured by its binding ability in a functional ELISA. Immobilized human IL2RA (CSB-MP011649HU3) at 2 μg/mL can bind this human IL2 protein, with the EC50 of 0.4992 to 0.6269 ng/mL.
IL-2 is a critical regulatory factor in the immune system, playing a significant role in both immune stimulation and immune suppression processes. IL-2 activates T cells, promoting cytokine production. It stimulates the proliferation of NK cells, enhancing their cytotoxic activity and cytokine production, and inducing the generation of LAK cells. IL-2 also facilitates B cell proliferation and antibody secretion. Additionally, IL-2 activates macrophages. An increasing body of research indicates that using low-dose IL-2 to selectively activate IL-2Rα holds great potential in the treatment of autoimmune diseases. Developing active and stable IL-2 proteins, as well as structurally stable high-affinity IL-2 receptor complexes, is of significant importance for in vitro studies of the interaction between IL-2 and IL-2 receptors, the generation of antibodies in immunological animals, and the in vitro screening of antibodies.
