AKT3 Antibody, FITC conjugated

Code CSB-PA15929C0Rb
Size US$299
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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) AKT3 Polyclonal antibody
Uniprot No. Q9Y243
Target Names AKT3
Alternative Names Akt3 antibody; AKT3 kinase antibody; AKT3_HUMAN antibody; DKFZp434N0250 antibody; MPPH antibody; PKB gamma antibody; PKBG antibody; PRKBG antibody; Protein kinase Akt-3 antibody; Protein Kinase AKT3 antibody; Protein kinase B gamma antibody; RAC gamma antibody; RAC gamma serine/threonine protein kinase antibody; RAC-gamma serine/threonine-protein kinase antibody; RAC-PK-gamma antibody; RACPK Gamma antibody; Serine threonine protein kinase Akt 3 antibody; Serine threonine protein kinase Akt3 antibody; STK 2 antibody; STK-2 antibody; STK2 antibody; V akt murine thymoma viral oncogene homolog 3 (protein kinase B; gamma) antibody; V akt murine thymoma viral oncogene homolog 3 antibody; V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human RAC-gamma serine/threonine-protein kinase protein (1-164AA)
Immunogen Species Homo sapiens (Human)
Conjugate FITC
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
Gene References into Functions
  1. The authors have found that overexpression of RIZ1 in HEK293 cells reduced the expression of Akt3 protein. PMID: 29367689
  2. Akt3 isoform expression in triple negative breast cancer.AKT3 splice variant lacking serine-472 phosphorylation site promotes apoptosis and suppresses mammary tumorigenesis. PMID: 29038347
  3. FEZF1-AS1 promoted multiple myeloma cells proliferation through regulating miR-610/Akt3 axis. PMID: 29864963
  4. these results suggested that this newly identified miR497/AKT3 signaling pathway may contribute to papillary thyroid cancer (PTC)occurrence and progression. These findings provide novel potential therapeutic targets for the therapy of PTC PMID: 28849051
  5. miR-30a-3p may be a promising biomarker for the early screening of high-risk populations and early diagnosis of Lung adenocarcinoma (LUAD). Our studies provide insights into identifying novel potential biomarkers for diagnosis and prognosis of LUAD PMID: 28791371
  6. these findings illustrated that cir-ZNF609 took part in the onset of HSCR through the crosstalk with AKT3 by competing for shared miR-150-5p. PMID: 27903978
  7. Results found that TR4 promotes the AKT3 expression through transcriptional regulation to drive the EMT phenotype and enhance the seminoma cell proliferation and invasion. PMID: 29197138
  8. Akt1-Akt3 activity (according to Thr308 phosphorylation) is not associated with proliferative processes in the tumor tissue of the thyroid. PMID: 29235817
  9. The results of this study indicated AKT isoforms have different roles and downstream substrates in glioblastoma. and they indicate AKT3 delays tumor progression. PMID: 27422127
  10. Report frequency of genetic variation in Akt3 and discuss link to disease. PMID: 28931550
  11. AKT3 Splice Variants are associated with HPV-Positive Oropharyngeal Cancers. PMID: 28733453
  12. Results show that AKT3 influences outcome of pneumococcal meningitis in human patients; validated the findings in a mouse experimental pneumococcal meningitis model. PMID: 27193124
  13. High AKT3 expression is associated with triple-negative breast cancer. PMID: 28160548
  14. This study showed that activating mutations of AKT3 are associated with a much broader spectrum of developmental brain disorders in children, with several clinical phenotypes determined partially by the type of mutation and level of mosaicism. PMID: 28969385
  15. MiR-511-3p may serve as a prognostic factor and tumor suppressor in prostate cancer, very likely through inverse regulation of its downstream target gene of AKT3. PMID: 28624527
  16. Studies found AKT3 to be important in coordinating mitochondrial biogenesis with growth factor-induced increase in cellular energy demands. This isoform also plays an important role in platelet activation and thrombosis. [review] PMID: 26953242
  17. miR-29b prevents angiogenesis/tumorigenesis in breast cancer cell by targeting Akt3 and inducing VEGF and C-myc arrest in breast cancer cells. PMID: 28365400
  18. AKT3 expression is markedly upregulated in AKT inhibitor-resistant cells. Induction of AKT3 is regulated epigenetically by the bromodomain and extra terminal domain proteins. Importantly, knockdown of AKT3, but not AKT1 or AKT2, in resistant cells restores sensitivity to MK2206. PMID: 27297869
  19. miR-15b-5p is a critical regulator of human EC proliferation and migration by targeting the AKT3 pathway. PMID: 28254819
  20. Akt3 constitutively suppresses macropinocytosis in macrophages through a novel WNK1/SGK1/Cdc42 pathway. PMID: 28389565
  21. partial co-localization of AKT3 with AURKB was observed during anaphase. Overall, this study suggests that AKT3 could repress the antiproliferative effects of AURKi, with a novel activity particularly suppressing the aneuploidy induction. PMID: 28028179
  22. A C119S Akt3 mutant was hypomorphic for all downstream phenotypes shown by wild-type Akt3. This study documents isozyme-specific and chemical redox signal-personalized physiological responses. PMID: 28114274
  23. the mitochondrial oxygen consumption rate was significantly reduced in Akt3-knockdown cancer cell lines PMID: 26972278
  24. our findings indicate that the expression levels of P-GP, MYC, caspase-8, and AKT3 are candidate biomarkers of cell sensitivity to PLKis. PMID: 27699933
  25. miR-29a could act as a tumor suppressor in PTC by targeting AKT3 and that miR-29a may potentially serve as an anti-tumor agent in the treatment of papillary thyroid carcinoma. PMID: 26482618
  26. showed that miR-20b was down-regulated in the retina and retinal endothelial cells in diabetic rats, with a correlated up-regulation of VEGF and AKT3 PMID: 27421659
  27. The siRNA-induced AKT3 and PI3KCA mRNA knockdown. PMID: 26902608
  28. Bioinformatics analysis further revealed cyclin D2 (CCND2) and AKT3, putative tumor promoters, as potential targets of miR610. Data from reporter assays showed that miR610 directly binds to the 3'untranslated PMID: 26782072
  29. Data show that elevated expression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs), Akt3 protein, and tumor suppressor protein p53 (p53) in ovarian serous adenocarcinoma tissues are an indication of more advanced disease and worse prognosis. PMID: 27629740
  30. Perhaps Bcl-2 down-regulation and Akt-3 up-regulation can be linked with survival signals in A549 cell line. We can conclude that Bcl-2 and Akt-3 might be therapeutic targets to inhibit cell proliferation in NSCLC. PMID: 27072269
  31. High AKT3 expression is associated with ovarian cancer. PMID: 26512921
  32. AKT3 has a role in prostate cancer proliferation through regulation of Akt, B-Raf, and TSC1/TSC2 PMID: 26318033
  33. mirn424 has a role in inhibiting Akt3/E2F3 axis and tumor growth in hepatocellular carcinoma PMID: 26315541
  34. Akt1 and Akt2 activated both SREBP-1 and SREBP-2, whereas Akt3 upregulated SREBP-1 to enhance hepatitis C virus translation. PMID: 26855332
  35. Flot-2 exerts a pro-neoplastic role in NPC and is involved in tumor progression and metastasis. Moreover, Flot-2 exerts its role through NF-kappaB and PI3K/Akt3 signaling. PMID: 26206082
  36. Downregulation of AKT3 increases migration and metastasis in triple negative breast cancer cells by upregulating S100A4. PMID: 26741489
  37. These findings indicate that AKT3 upregulation may cause focal malformations of cortical development. PMID: 25091978
  38. These findings suggest a central AKT-FOXG1-reelin signaling pathway in focal malformations of cortical development and support pathway inhibitors as potential treatments or therapies for some forms of focal epilepsy. PMID: 26523971
  39. Data show that both serine/threonine phosphatases PHLPP and dephosphorylated the physiological substrates of Akt1 and Akt3 with similar efficiencies. PMID: 26427440
  40. miR-582-5p regulated the progression of hepatocellular carcinoma through directly inhibiting the expression of CDK1 and AKT3, and indirectly inhibiting the expression of cyclinD1 PMID: 26002580
  41. A 1q44 deletion involving only AKT3 in a boy and his father, is reported. PMID: 25424989
  42. MiR-22 induces EPC senescence by downregulating AKT3 expression. PMID: 25323119
  43. findings identify PI3K/AKT pathway mutations as an important cause of epileptogenic brain malformations and establish megalencephaly, hemimegalencephaly, and focal cortical dysplasia as part of a single pathogenic spectrum. PMID: 25722288
  44. AKT3, PI3KCA, and VEGFR2 silencing reduces the invasiveness of glioblastoma multiforme T98G cells. PMID: 25501707
  45. The individual contribution of each Akt isoform in p120 RasGAP fragment N-mediated cell protection against Fas ligand induced cell death, was investigated. PMID: 25246356
  46. Data suggest that three isoforms of the serine/threonine protein kinase Akt (Akt1, Akt2, Akt3) regulate cell survival, cell growth, cell proliferation, and cell metabolism in breast cancer cells. [REVIEW] PMID: 25233414
  47. Akt3-expressing human glioblastoma cells had enhanced activation of DNA repair proteins, leading to increased DNA repair and subsequent resistance to radiation and temozolomide. PMID: 25737557
  48. The PI3K/AKT/mTOR pathway is distinguishable in SCLC genomic alterations. Therefore, a sequencing-based comprehensive analysis could stratify SCLC patients by potential therapeutic targets. PMID: 25122428
  49. Akt3 is a negative regulator of IGFBP-3 protein. PMID: 24942865
  50. Our study revealed AKT-3 amplification and deletions in 11% (9/82) and 13% (11/82) of triple-negative breast cancers, respectively. PMID: 24138071
  51. Mechanistic investigations uncovered opposing functions for different Akt isoforms in this regulation, where Akt1 promotes and Akt3 inhibits vascular tumor growth. PMID: 25388284
  52. miR-145 suppresses thyroid cancer growth and metastasis and targets AKT3 PMID: 24781864
  53. Knockdown of AKT3 and/or PI3KCA genes in T98G cells led to a significant reduction in cell viability, the accumulation of subG1-phase cells and, a reduced fraction of cells in the S and G2/M phases. PMID: 24967401
  54. miR-489 inhibited cisplatin resistance and cell growth, and promotes apoptosis by suppressing Akt3 expression. PMID: 24686007
  55. Inactivation of AKT3 in ErbB2+/ERalpha- breast cancer cells results in decreased expression of ErbB2 and ErbB3, but increased expression of ERalpha. PMID: 24463007
  56. Induction of transgenic Akt3 constitutively activates pathways for survival by counteracting motor neuronal degeneration in patients with amyotrophic lateral sclerosis. PMID: 23873136
  57. Experimental validation indicate that AKT1, AKT2 and AKT3 proteins may all be novel unfavorable prognostic factors for patients with hepatocellular carcinoma. PMID: 24247267
  58. Various AKT3 mutations are important in neurological disorders, such as microcephaly, hemimegalencephaly, and megalencephaly syndromes PMID: 24039187
  59. detected and measured all three AKT isoforms 1, 2 and 3 to enable the study of the multiple and variable roles that these isoforms play in AKT breast tumorigenesis PMID: 23929892
  60. IWS1, an RNA processing regulator, is phosphorylated by Akt3 and Akt1 at Ser720/Thr721 in lung cancer PMID: 24462114
  61. targeting Akt3 and downstream signaling may be an effective approach to inhibit growth of triple-negative breast cancer (TNBC0. PMID: 24335962
  62. Akt3 coimmunoprecipitates with Ago2. PMID: 23603119
  63. Duplication of AKT3 causes macrocephaly. PMID: 23794269
  64. AKT isoforms 1 and 3 promote basal as well as EGF-induced trophoblast migration. PMID: 23303682
  65. AKT3, ANGPTL4, eNOS3, and VEGFA associations with high altitude sickness in Han and Tibetan Chinese at the Qinghai-Tibetan Plateau. PMID: 22729570
  66. Exome sequencing and mass spectrometry analysis in paired brain-blood samples from individuals with hemimegalencephaly (20 cases) identified de novo somatic mutations in 30% of affected individuals in the PIK3CA, AKT3 and MTOR genes. PMID: 22729223
  67. identified mutations in AKT3, PIK3R2 and PIK3CA in 11 unrelated families with megalencephaly-capillary malformation and megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndromes PMID: 22729224
  68. In hepatocellular carcinoma miR-519d is up-regulated by p53 and DNA hypomethylation and targets CDKN1A/p21, PTEN, AKT3 and TIMP2. PMID: 22262409
  69. Resveratrol down-regulates the protein cyclin D1 and, in a concentration dependent manner, the phosphorylation levels of protein kinase B and glycogen synthase kinase-3beta in OVCAR-3 ovarian cancer cells. PMID: 22234583
  70. Transcriptional activation of the Akt3 pathway indicates that it is involved in lumbar disc degeneration. PMID: 21590431
  71. This study identifies a trisomy on chromosome 1q, which encompasses AKT3, and a separate activating AKT3 somatic mutation in patients with the developmental brain disorder hemimegalencephaly. PMID: 22500628
  72. The prognostic impact of Akt (Akt1) phosphorylated at threonine308 and serine473, Akt2, Akt3, PI3K and PTEN, alone and in coexpression with ER and PgR in non-gastrointestinal stromal tumor soft tissue sarcomas. PMID: 22107784
  73. These findings suggest an important role for Akt3 in the regulation of RCAS1 and VEGF secretion in ovarian cancer cells. PMID: 21351097
  74. Our data demonstrate that Akt1 and notably Akt3 regulate proliferation, survival, migration and EGF-mediated signal transduction in NSCLC-derived DTC PMID: 21777670
  75. Akt3 plays an important and distinct role in platelet activation and in thrombosis. PMID: 21821713
  76. We did not find any mutation in the GNAQ, AKT3, and PIK3R1 genes in various types of thyroid cancer. PMID: 21487925
  77. Akt3 was required for anchorage-independent growth of transformed astrocytes and human glioma cells PMID: 21507933
  78. Expression of matrix metalloproteinase-13 is controlled by IL-13 via PI3K/Akt3 and PKC-delta in normal human dermal fibroblasts. PMID: 21191416
  79. No AKT3 E17K mutation was detected in the 73 squamous cell carcinomas of the lung PMID: 20980808
  80. Mutant B-RAF melanoma cells ectopically expressing a constitutively activated form of Akt3 or endogenously expressing mutant Akt3 were protected from apoptosis induced by B-RAF knockdown or PLX4720 treatment. PMID: 20647317
  81. Studies indicate that three different isoforms Akt1, Akt2, and Akt3 have distinct expression patterns and functions. PMID: 20398329
  82. We evaluated the presence of mutations in PIK3CA, AKT1, AKT2, AKT3, PTEN, and PDPK1 genes in 83 papillary thyroid carcinomas PMID: 20186503
  83. In breast cancer AKT3 amplifications and AKT1 and AKT2 deletions were seen by FISH PMID: 20102399
  84. Our data suggest that Akt2 and Akt3 play an important role in the viability of human malignant glioma cells PMID: 20167810
  85. Akt3 was located in the nucleus and nuclear membrane of human cell lines. PMID: 20018949
  86. results show that a defect in the ability of insulin to activate Akt-2 and -3 may explain the impaired insulin-stimulated glucose transport in insulin resistance PMID: 12663464
  87. The regulation by Akt3 was found to be due to two specific regions in the Fra-1 regulatory sequence which match Sp1 consensus sites PMID: 12692267
  88. The above results indicate that PKB-Ser-473 and FAK-Tyr phosphorylation stimulated by TGF-beta1 are both dependent on cell adhesion. PMID: 14637150
  89. Targeted reduction of Akt3 activity with siRNA or by expressing active PTEN protein stimulated apoptotic signaling, which reduced cell survival by increasing apoptosis rates thereby inhibiting melanoma tumor development. PMID: 15466193
  90. Dysregulation of the PI 3-K/AKT/PTEN pathway may contribute to early head neck squamous cell carcinoma tumorigenesis. PMID: 15543611
  91. Rac1, PI3 kinase, and Akt3 have roles in an anti-apoptotic pathway triggered by ALS2 that antagonizes SOD1 mutant-induced motoneuronal cell death PMID: 15579468
  92. activation of Akt signaling results in progression from adaptive to maladaptive cardiac hypertrophy PMID: 15698844
  93. in breast cancer patients, Akt activation is associated with tumour proliferation and poor prognosis, particularly in the subset of patients with ErbB2-overexpressing tumours PMID: 15987444
  94. AKT3 is highly expressed in 19 of 92 primary ovarian tumors, consistent with AKT3 playing a key role in the genesis of at least one subset of ovarian cancers. PMID: 17178867
  95. AKT3 represents an excellent candidate for developmental human MIC and ACC, and we suggest that haploinsufficiency causes both postnatal MIC and ACC. PMID: 17668379
  96. PKB[alpha] and/or PKB[gamma] and not PKB[beta] alone are involved in gemcitabine resistance mechanisms. PMID: 17895832
  97. Akt3 and mutant V600E B-Raf cooperate to promote early melanoma development. PMID: 18451171
  98. the first report of AKT mutations in melanoma, and the initial identification of an AKT3 mutation in any human cancer lineage PMID: 18813315
  99. Bcr-Abl represses the expression of PHLPP1 and PHLPP2 and continuously activates Akt1, -2, and -3 via phosphorylation on Ser-473, resulting in the proliferation of CML cells PMID: 19261608
  100. Findings indicate that AP-1 has an important function in pancreatic cancer cells and provide evidence for a previously unknown Akt-mediated mechanism of c-Jun activation. PMID: 19435822

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Involvement in disease Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 2 (MPPH2)
Subcellular Location Nucleus, Cytoplasm, Membrane, Peripheral membrane protein
Protein Families Protein kinase superfamily, AGC Ser/Thr protein kinase family, RAC subfamily
Tissue Specificity In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Database Links

HGNC: 393

OMIM: 611223

KEGG: hsa:10000

STRING: 9606.ENSP00000263826

UniGene: Hs.498292

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