Recombinant Arabidopsis thaliana Potassium channel AKT2/3 (AKT2), partial

Code CSB-YP653630DOA
MSDS
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Source Yeast
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Code CSB-EP653630DOA
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Source E.coli
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Code CSB-EP653630DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP653630DOA
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Source Baculovirus
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Code CSB-MP653630DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
AKT2; AKT3; At4g22200; T10I14.30Potassium channel AKT2/3
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Highly selective and weak inward-rectifying potassium channel. Plays a role in both loading and unloading potassium into/from the phloem sap. Seems to control sugar loading into phloem via a voltage-dependent process. Blocked by physiological concentrations of external calcium and by external acidification. May interact with the cytoskeleton or with regulatory proteins. Dephosphorylation by PP2CA not only leads to the inhibition of potassium currents but also to an increase of the voltage-dependence of the channel. Regulated by the CBL4/CIPK6 calcium sensor/protein kinase complex via a kinase interaction-dependent but phosphorylation-independent translocation of the channel to the plasma membrane.
Gene References into Functions
  1. the outward-rectifying potassium-selective channel GORK limits the action potential amplitude and duration, while the weakly-rectifying channel AKT2 affects membrane excitability. PMID: 29561764
  2. AKT2 subunit containing K(+) channels mediate sodium chloride-induced depolarization of root cells. PMID: 27043750
  3. CBL4 together with the interacting protein kinase CIPK6 modulates the activity and plasma membrane targeting of the K(+) channel AKT2. PMID: 21445098
  4. Data discuss the existence of multiple post-translational modification steps that work in concert to convert AKT2 from an inward-rectifying into a non-rectifying K (+) channel. PMID: 21445013
  5. the AKT2/3 subunit constitutes the Ca2+ sensitivity of the guard cell K+ uptake channel PMID: 15824192
  6. The role of 2 phosphorylation sites on the switch between gating modes of AKT2 are described. PMID: 16297070
  7. AKT2-transformed tobacco mesophyll cells displayed inward-rectifying, rather than voltage-independent K+ channels. PMID: 17146665

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Targeted to the cell membrane when interacting with CIPK6 and CBL4.
Protein Families
Potassium channel family, Plant (TC 1.A.1.4) subfamily
Tissue Specificity
Expressed mainly in the phloem tissues throughout the plant but also, at a lower level, in leaf epiderm, mesophyll and guard cells.
Database Links

KEGG: ath:AT4G22200

STRING: 3702.AT4G22200.1

UniGene: At.1970

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