Recombinant Arabidopsis thaliana Potassium channel AKT2/3(AKT2) ,partial

Code CSB-YP653630DOA
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Source Yeast
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Code CSB-EP653630DOA
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Source E.coli
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Code CSB-EP653630DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP653630DOA
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Source Baculovirus
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Code CSB-MP653630DOA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names AKT2
Uniprot No. Q38898
Alternative Names AKT2; AKT3; At4g22200; T10I14.30Potassium channel AKT2/3
Species Arabidopsis thaliana (Mouse-ear cress)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Highly selective and weak inward-rectifying potassium channel. Plays a role in both loading and unloading potassium into/from the phloem sap. Seems to control sugar loading into phloem via a voltage-dependent process. Blocked by physiological concentrations of external calcium and by external acidification. May interact with the cytoskeleton or with regulatory proteins. Dephosphorylation by PP2CA not only leads to the inhibition of potassium currents but also to an increase of the voltage-dependence of the channel. Regulated by the CBL4/CIPK6 calcium sensor/protein kinase complex via a kinase interaction-dependent but phosphorylation-independent translocation of the channel to the plasma membrane.
Gene References into Functions
  1. the outward-rectifying potassium-selective channel GORK limits the action potential amplitude and duration, while the weakly-rectifying channel AKT2 affects membrane excitability. PMID: 29561764
  2. AKT2 subunit containing K(+) channels mediate sodium chloride-induced depolarization of root cells. PMID: 27043750
  3. CBL4 together with the interacting protein kinase CIPK6 modulates the activity and plasma membrane targeting of the K(+) channel AKT2. PMID: 21445098
  4. Data discuss the existence of multiple post-translational modification steps that work in concert to convert AKT2 from an inward-rectifying into a non-rectifying K (+) channel. PMID: 21445013
  5. the AKT2/3 subunit constitutes the Ca2+ sensitivity of the guard cell K+ uptake channel PMID: 15824192
  6. The role of 2 phosphorylation sites on the switch between gating modes of AKT2 are described. PMID: 16297070
  7. AKT2-transformed tobacco mesophyll cells displayed inward-rectifying, rather than voltage-independent K+ channels. PMID: 17146665

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Subcellular Location Endoplasmic reticulum membrane, Multi-pass membrane protein
Protein Families Potassium channel family, Plant (TC 1.A.1.4) subfamily
Tissue Specificity Expressed mainly in the phloem tissues throughout the plant but also, at a lower level, in leaf epiderm, mesophyll and guard cells.
Database Links

KEGG: ath:AT4G22200

STRING: 3702.AT4G22200.1

UniGene: At.1970

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