AKT2 Antibody

Code CSB-PA653630XA01DOA
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Product Details

Full Product Name Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AKT2 Polyclonal antibody
Uniprot No. Q38898
Target Names AKT2
Alternative Names AKT2 antibody; AKT3 antibody; At4g22200 antibody; T10I14.30Potassium channel AKT2/3 antibody
Raised in Rabbit
Species Reactivity Arabidopsis thaliana
Immunogen Recombinant Arabidopsis thaliana AKT2 protein
Immunogen Species Arabidopsis thaliana (Mouse-ear cress)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Made-to-order

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Target Data

Function Highly selective and weak inward-rectifying potassium channel. Plays a role in both loading and unloading potassium into/from the phloem sap. Seems to control sugar loading into phloem via a voltage-dependent process. Blocked by physiological concentrations of external calcium and by external acidification. May interact with the cytoskeleton or with regulatory proteins. Dephosphorylation by PP2CA not only leads to the inhibition of potassium currents but also to an increase of the voltage-dependence of the channel. Regulated by the CBL4/CIPK6 calcium sensor/protein kinase complex via a kinase interaction-dependent but phosphorylation-independent translocation of the channel to the plasma membrane.
Gene References into Functions
  1. the outward-rectifying potassium-selective channel GORK limits the action potential amplitude and duration, while the weakly-rectifying channel AKT2 affects membrane excitability. PMID: 29561764
  2. AKT2 subunit containing K(+) channels mediate sodium chloride-induced depolarization of root cells. PMID: 27043750
  3. CBL4 together with the interacting protein kinase CIPK6 modulates the activity and plasma membrane targeting of the K(+) channel AKT2. PMID: 21445098
  4. Data discuss the existence of multiple post-translational modification steps that work in concert to convert AKT2 from an inward-rectifying into a non-rectifying K (+) channel. PMID: 21445013
  5. the AKT2/3 subunit constitutes the Ca2+ sensitivity of the guard cell K+ uptake channel PMID: 15824192
  6. The role of 2 phosphorylation sites on the switch between gating modes of AKT2 are described. PMID: 16297070
  7. AKT2-transformed tobacco mesophyll cells displayed inward-rectifying, rather than voltage-independent K+ channels. PMID: 17146665

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Subcellular Location Endoplasmic reticulum membrane, Multi-pass membrane protein
Protein Families Potassium channel family, Plant (TC 1.A.1.4) subfamily
Tissue Specificity Expressed mainly in the phloem tissues throughout the plant but also, at a lower level, in leaf epiderm, mesophyll and guard cells.
Database Links

KEGG: ath:AT4G22200

STRING: 3702.AT4G22200.1

UniGene: At.1970

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