CD40 Research Reagents

CD40, also known tumor necrosis factor receptor superfamily member 5 ( TNFRSF5), is a 45-50 kDa type I glycoprotein and belongs to tumor necrosis factor receptor (TNFR) superfamily. CD40 is widely expressed by antigen presenting cells (APCs) or all stages of B cells. The ligand of CD40 is CD154 (also known as CD40L), which is a type II transmembrane protein with a variable molecular weight between 32-39 kDa. CD40L is mainly expressed on activated CD4+ T cells and is a member of TNF superfamily. Like many other members of the TNFR superfamily, CD40 are also divided into membrane-anchored CD40 (mCD40) and soluble CD40 (sCD40). sCD40 retains the ability to bind to CD40L, and is an important negative regulator of the effect of CD40-CD40L.

CD40/CD40L interaction plays an important role in immune regulation. CD40/CD40L belongs to receptor-ligand families: TNFR and TNF family. Families are characterized by structural homologies, clustered chromosome location, and shared signal transduction pathways. In addition, families usually overlap biological events like cell growth, differentiation and death. The interaction of CD40-CD40L can produce a wide range of biological effects, which are closely related to the occurrence, development and immune diseases of atherosclerosis, making it a promising therapeutic target for these diseases. Animal experiments and in vitro experiments show that anti-CD40 monoclonal antibody has obvious curative effect on the treatment of AS, tumor and post-transplant rejection.

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Popular Product

Recombinant Human Tumor necrosis factor receptor superfamily member 5(CD40),partial (Active) (CSB-MP004936HU1)

Validated Data

Purity determined
by
SDS-PAGE

Greater than 92% as determined by SDS-PAGE.

The binding activity of CD40-CD40L validated by functional ELISA

Immobilized CD40 at 2 μg/ml can bind CD40L (CSB-MP004937HU3), the EC₅₀ is 3.112-3.858 ng/ml.

The binding activity of CD40-CD40L validated
by LSPR

Human CD40 protein captured on COOH chip can bind Human CD40L protein with an affinity constant of 2.06 nM as detected by LSPR Assay.