DLAT (dihydrolipoamide S-acetyltransferase) is a protein found in mitochondria and constitutes a component of the α-ketoglutarate dehydrogenase complex. It contains an active site that catalyzes the acetyl transfer reaction between acetyl-CoA and α-ketoglutarate. DLAT plays a significant role in the tricarboxylic acid cycle (TCA cycle), facilitating a crucial metabolic step between acetyl-CoA generated from food breakdown and α-ketoglutarate in the TCA cycle. It transfers acetyl-CoA to α-ketoglutarate, aiding its entry into the TCA cycle, thereby contributing to the generation of NADH and FADH2 necessary for cellular energy production. The activity and function of DLAT are vital in maintaining normal energy metabolism, particularly within the mitochondria of cells. Understanding the role of DLAT in energy metabolism is crucial for comprehending mitochondrial dysfunction and related disorders, such as metabolic diseases.
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP804374MOa0 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Dlat.
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP804374MOa0 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Dlat.
The following Dlat reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
Dlat Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA006926GA01HU | DLAT Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA445587 | DLAT Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA084734 | DLAT Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA006926LA01HU | DLAT Antibody |
Human | ELISA, WB, IP |
Dlat Antibodies for Mus musculus (Mouse)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA804374ZA01MO | Dlat Antibody |
Mus musculus | ELISA, WB (ensure identification of antigen) |
Dlat Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP006926HU CSB-BP006926HU CSB-MP006926HU CSB-EP006926HU-B |
Recombinant Human Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (DLAT), partial |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP006926HU | Recombinant Human Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (DLAT), partial |
E.coli |
Dlat Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP804374MO | Recombinant Mouse Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (Dlat) |
Yeast |
CSB-EP804374MO CSB-BP804374MO CSB-EP804374MO-B |
Recombinant Mouse Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (Dlat) |
E.coli Baculovirus In Vivo Biotinylation in E.coli |
CSB-EP804374MOa0 | Recombinant Mouse Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (Dlat) |
E.coli |
CSB-MP804374MO | Recombinant Mouse Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (Dlat) |
Mammalian cell |