PIK3CA

PIK3CA, or phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, alias PI3K-alpha. PIK3CA is encoded by the human PIK3CA gene, which is located on chromosome 3 in the q26.3 region, contains 21 exons, encodes 1,068 amino acids, and produces a group of proteins that are 124 kD long.
The mechanism of action of PIK3CA involves Catalyzing the conversion of the membrane lipid phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] to the second messenger PI(3,4,5)P3 (also known as PIP3), which activates the AKT-mTOR signaling pathway and promotes cell survival, proliferation, growth, and motility.
PIK3CA is one of the most commonly mutated genes in cancer, and its activating mutation has been observed in breast, endometrial, and bladder cancers, colorectal cancer and other cancers, which are involved in tumorigenesis, progression and metastatic colonization. Mutations in the PIK3CA gene lead to the upregulation of the PI3K/AKT pathway level, which in turn is involved in tumorigenesis.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

PIK3CA Recombinant Monoclonal Antibody (CSB-RA578819A0HU)

Validated Data

Western Blot
Positive WB detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate
All lanes: PIK3CA antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 125 kDa
Observed band size: 110 kDa

Immunofluorescence staining of Hela Cells with CSB-RA578819A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

The following PIK3CA reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

PIK3CA Antibodies

PIK3CA Proteins

PIK3CA ELISA Kits

PIK3CA Antibodies

PIK3CA Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA017998GA01HU	PIK3CA Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA003761	PIK3CA Antibody	Human,Mouse	WB, IHC, ELISA
CSB-PA017998LA01HU	PIK3CA Antibody	Human	ELISA, IHC, IF
CSB-PA017998LC01HU	PIK3CA Antibody, FITC conjugated	Human
CSB-PA017998LD01HU	PIK3CA Antibody, Biotin conjugated	Human	ELISA
CSB-RA578819A0HU	PIK3CA Recombinant Monoclonal Antibody	Human, Mouse	ELISA, WB, IF

PIK3CA Proteins

PIK3CA Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP017998BO CSB-EP017998BO CSB-BP017998BO CSB-MP017998BO CSB-EP017998BO-B	Recombinant Bovine Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

PIK3CA Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP017998HU CSB-BP017998HU CSB-MP017998HU CSB-EP017998HU-B	Recombinant Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP017998HU	Recombinant Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA), partial	E.coli

PIK3CA Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP017998MO CSB-EP017998MO CSB-BP017998MO CSB-MP017998MO CSB-EP017998MO-B	Recombinant Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (Pik3ca), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli