TNFRSF9, tumor necrosis factor receptor superfamily member 9, alias CD137, 4-1BB, is a type I transmembrane protein belonging to the tumor necrosis factor receptor superfamily, which is mainly expressed on the surface of activated T cells, but also in B cells, monocytes, and various transformed cell lines.
TNFRSF9 protein binds to ligand 4-1BBL (TNFSF9), which activates the downstream NF-κB signaling pathway and promotes clonal proliferation, survival and development of T cells. TNFRSF9 plays a critical role in the immune response, especially in enhancing T cell-mediated immune responses and regulating cytotoxic T cell activity.
TNFRSF9 expression is also associated with a variety of diseases, including autoimmune diseases and tumors, and is a potential target for immunotherapy.
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized TNFRSF9 at 2 μg/ml can bind TNFSF9(CSB-MP023997HU1), the EC50 is 1.011-2.429 ng/mL.
TNFRSF9 Antibody (CSB-PA08109A0Rb)
Validated Data
Western Blot
Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
All lanes: TNFRSF9 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 65 kDa
Immunofluorescent analysis of A549 cells using CSB-PA08109A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
The following TNFRSF9 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
TNFRSF9 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA011218 | TNFRSF9 Antibody | Human | WB, IF, ELISA |
CSB-PA983768 | TNFRSF9 Antibody | Human | ELISA,IHC |
CSB-PA718806 | TNFRSF9 Antibody | Human | IHC,ELISA |
CSB-PA08109A0Rb | TNFRSF9 Antibody | Human | ELISA, WB, IF |
CSB-PA08109B0Rb | TNFRSF9 Antibody, HRP conjugated | Human | ELISA |
CSB-PA08109C0Rb | TNFRSF9 Antibody, FITC conjugated | Human | |
CSB-PA08109D0Rb | TNFRSF9 Antibody, Biotin conjugated | Human | ELISA |
CSB-RA023984A1HU | TNFRSF9/4-1BB Recombinant Monoclonal Antibody | Human | ELISA |
TNFRSF9 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-AP001951HU | Recombinant Human Tumor necrosis factor receptor superfamily member 9 protein (TNFRSF9),Partial (Active) | E.Coli |
CSB-AP005131HU | Recombinant Human Tumor necrosis factor receptor superfamily member 9 (TNFRSF9), partial (Active) | Mammalian cell |
CSB-AP005141HU | Recombinant Human Tumor necrosis factor receptor superfamily member 9 (TNFRSF9), partial (Active) | Mammalian cell |
CSB-AP005151HU | Recombinant Human Tumor necrosis factor receptor superfamily member 9 (TNFRSF9), partial (Active) | Mammalian cell |
CSB-MP023984HU1 | Recombinant Human Tumor necrosis factor receptor superfamily member 9 (TNFRSF9), partial (Active) | Mammalian cell |
TNFRSF9 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-CF023984MO | Recombinant Mouse Tumor necrosis factor receptor superfamily member 9 (Tnfrsf9) | in vitro E.coli expression system |
CSB-YP023984MO1 CSB-EP023984MO1 CSB-BP023984MO1 CSB-MP023984MO1 CSB-EP023984MO1-B |
Recombinant Mouse Tumor necrosis factor receptor superfamily member 9 (Tnfrsf9), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TNFRSF9 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL023984HU | Human Tumor necrosis factor receptor superfamily member 9(TNFRSF9) ELISA kit | serum, plasma, tissue homogenates | 1.95 pg/mL |
TNFRSF9 ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL023984MO | Mouse Tumor necrosis factor receptor superfamily member 9(TNFRSF9) ELISA kit | serum, plasma, tissue homogenates | 3.9 pg/ml |