TOP2, as a topoisomerase II, plays a crucial role in DNA replication and transcription. Its complex protein structure endows it with specific catalytic and DNA binding abilities, enabling it to accurately recognize and bind to DNA. During DNA replication, TOP2 ensures the smooth progression of the replication process by solving the "topological problems" caused by topological structure changes. Similarly, during transcription, TOP2 also plays a similar role, ensuring the smooth transcription of RNA polymerase. In addition, the activity of TOP2 is regulated by various factors and signaling molecules, and abnormal regulation can lead to the occurrence of related diseases.
TOP2A Recombinant Monoclonal Antibody
Validated Data
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: TOP2A antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 175, 178, 179, 183 kDa
Observed band size: 175 kDa
IHC image of CSB-RA696677A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
The following TOP2 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
TOP2 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA024060GA01HU | TOP2A Antibody |
Human | ELISA,WB,IHC,IF |
CSB-PA248815 | Phospho-TOP2A (Thr1343) Antibody |
Human | ELISA,WB |
CSB-PA438319 | TOP2A (Ab-1343) Antibody |
Human | ELISA,WB |
CSB-PA105332 | TOP2A Antibody |
Human | ELISA,WB,IHC,IF |
CSB-PA949180 | Phospho-TOP2A (Ser1106) Antibody |
Human,Mouse | ELISA,WB,IHC |
CSB-PA004317 | TOP2A Antibody |
Human,Monkey | WB, IHC, IF, ELISA |
CSB-PA004663 | TOP2A Antibody |
Human,Mouse | WB, ELISA |
CSB-PA050284 | Phospho-TOP2A (T1343) Antibody |
Human | WB, ELISA |
CSB-PA050285 | Phospho-TOP2A (S1106) Antibody |
Human | WB, IHC, ELISA |
CSB-PA050286 | Phospho-TOP2A (S1525) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA050287 | TOP2A Antibody |
Human | WB, IHC, ELISA |
CSB-PA050288 | TOP2A Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA050289 | TOP2A Antibody |
Human | WB, IHC, ELISA |
CSB-PA062296 | TOP2A Antibody |
Human,Mouse,Rat | ELISA,IHC |
CSB-RA696677A0HU | TOP2A Recombinant Monoclonal Antibody |
Human, Mouse | ELISA, WB, IHC |
TOP2 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP024060HU CSB-EP024060HU CSB-BP024060HU CSB-MP024060HU CSB-EP024060HU-B |
Recombinant Human DNA topoisomerase 2-alpha (TOP2A), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |