Bovine Vascular endothelial growth factor A(VEGFA) ELISA kit

Code CSB-EL025833BO
Size 96T,5×96T,10×96T
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Product Details

Target Name
vascular endothelial growth factor A
Alternative Names
VEGFA ELISA kit; VEGFVascular endothelial growth factor A ELISA kit; VEGF-A ELISA kit; Vascular permeability factor ELISA kit; VPF ELISA kit
Uniprot No.
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
47 pg/mL-3000 pg/mL
11.75 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of bovine VEGFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 88102
1:2 Average % 92
Range % 87-92
1:4 Average % 90
Range % 85-94
1:8 Average % 92
Range % 84-96
The recovery of bovine VEGFA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 84-95
EDTA plasma (n=4) 104 97-111
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.147 1.988 2.068 1.940
1500 1.604 1.576 1.590 1.462
750 1.105 1.089 1.097 0.969
375 0.756 0.767 0.762 0.634
187.5 0.538 0.531 0.535 0.407
94 0.340 0.332 0.336 0.208
47 0.228 0.221 0.225 0.097
0 0.127 0.129 0.128  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Bovine VEGFA ELISA Kit was designed for the quantitative measurement of Bovine VEGFA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.

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I was wondering if you have a VEGF ELISA kit that I can test for both Human and Bovine samples? This is the Bovine code CSB-EL025833BO.

Thanks for your inquiry!
We suggest you use bovine kit CSB-EL025833BO to test bovine samples and human kit CSB-E11718h to test human samples because these kits are developed against different matrix. The antibodies used in the kits have different resistance to matrix interference.

Target Background

(From Uniprot)
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.
Gene References into Functions
  1. data for the first time demonstrate a calpain/PTP1B/VEGFR2 negative feedback loop in the regulation of VEGF-induced angiogenesis. Modulation of local PTP1B and/or calpain activities may prove beneficial in the treatment of impaired wound healing in diabetes. PMID: 27872190
  2. Studied the effect of cyclic uniaxial stretch (20%, 1 Hz), with and without the stimulation of vascular endothelial growth factor (VEGF), on sprouting angiogenesis by employing a stretchable three-dimensional cell culture model. PMID: 25376776
  3. Study shows that VEGFA mRNA in mammalian endothelial cells undergoes programmed translational readthrough (PTR) generating VEGF-Ax, an isoform containing a unique 22-amino-acid C terminus extension. PMID: 24949972
  4. Data indicate that superoxide dismutase (SOD) inhibited high glucose (HG)-induced expression of uPAR and VEGF in bovine retinal microvascular endothelial cell (REC). PMID: 23951261
  5. Exposure of endothelial cells to VEGF, high glucose, or hydrogen peroxide up-regulated the XBP1/IRE1 alpha and ATF6 arms of the unfolded protein response compared with untreated cells. PMID: 23395094
  6. Microvascular endothelial cells are more susceptible than aortic cells to advanced glycation end products-enhanced permeability and that AGE-enhanced permeability is dependent on VEGF expression induced by reactive oxygen species. PMID: 21296593
  7. VEGF supports germ cell survival and sperm production in bulls. PMID: 19633133
  8. analysis of polymorphisms of bovine VEGF gene and their associations with growth traits in Chinese cattle PMID: 20376703
  9. VEGF mRNA expression at estrus was higher than at the early I, early II and late luteal stages (P<0.05), whereas VEGF protein content was greatest at the early I luteal stage and decreased thereafter PMID: 20035107
  10. Alterations in the expression of VEGF-A and bFGF systems suggest that angiogenic factors are involved in abnormal placental development in cloned gestations, contributing to impaired fetal development and poor survival rates. PMID: 20198587
  11. Advanced glycation end products increase, through a protein kinase C-dependent pathway, expression in reignal endothelial cells; inhibited by gliclazide. PMID: 12126787
  12. VEGF signaling is regulated by atrial natriuretic peptide, which preserves endothelial cell tight junction functional morphology PMID: 12213803
  13. Airline pilots are good candidates to see if mutation in the VEGF promoter are responsible for some cases of amyotrophic lateral sclerosis. PMID: 12448348
  14. role in dowregulating Flk-1/KDR involves Cbl-mediated ubiquitination PMID: 12649282
  15. interaction with glucose-6-phosphate dehydrogenase is involved in angiogenesis PMID: 12777375
  16. Results suggest that leptin might elicit angiogenesis through vascular endothelial growth factor induction as well as pigment epithelium-derived factor suppression in pericytes. PMID: 12779248
  17. Shear stress and VEGF converge at the membrane receptor Flk-1 and these stimuli share the Flk-1/Cbl/Akt pathway in activating IKK activation. PMID: 14551058
  18. Tumor cells activate confluent, quiescent EC, promoting survival, phenotypic, and gene expression changes. Of importance, VEGF antagonism converts tumor-conditioned medium from protective to endothelial cell-damaging effects. PMID: 15075216
  19. MGP plays a role in endothelial cell function, by increasing transforming growth factor-beta1 activity and stimulating VEGF expression PMID: 15456771
  20. placenta growth factor expression is regulated by both VEGF and hyperglycaemia via VEGFR-2 PMID: 15501243
  21. VEGF has a role in increasing paracellular transport PMID: 15501249
  22. Thrombospondin-1 and -2 were coordinately expressed in the extravascular compartment of the ovary during early follicle development. VEGF was inversely expressed, with expression increasing as follicles developed. PMID: 15616224
  23. the activation of VEGFR-1 and VEGFR-2 heterodimer (VEGFR-1/R-2) is essential for PGI(2) synthesis mediated by VEGF-A(165) and VEGF-A(121), which cannot be reproduced by the parallel activation of VEGFR-1 and VEGFR-2 homodimers with corresponding agonists PMID: 15637071
  24. Reciprocally, adenovirus-mediated overexpression of Cyr61 in cells cultured under static conditions increased the expression of VEGF, alpha(v)-integrin, and SM alpha-actin. PMID: 15649872
  25. peroxynitrite causes increased expression of VEGF in vascular endothelial cells by a process that requires the activation of STAT3 PMID: 16257644
  26. ROS induce expression not only of VEGF but also of VEGFR2. VEGFR2 increase by ROS is mainly driven through a NF-kappaB-dependent pathway. PMID: 16899768
  27. expression of VEGF isoforms may be associated with characterization of the preovulatory phenotype during follicle development in the bovine ovary PMID: 17043385
  28. Gab1 is a novel critical regulatory component of endothelial cell migration and capillary formation with a key role in the activation of VEGF-evoked signaling pathways required for angiogenesis PMID: 17178724
  29. The results provide support for a role for VEGF in the primary to secondary follicle transition. PMID: 17290425
  30. This study reports the temporal changes in vascular endothelial growth factor A (VEGFA), fibroblast growth factor 2 (FGF2) and osteonectin during the follicular-luteal transition and corpus luteum development in the cow. PMID: 17360962
  31. a new molecular cascade, operating in retinal bovine pericytes and involving PKCbetaII, the mRNA-stabilizing protein HuR. and VEGF. In particular we show that PKCbetaII activation is responsible, through the RNA-binding protein HuR PMID: 18206386
  32. bFGF and VEGF control the corpus luteum formation and function. PMID: 18213648
  33. VEGF has an effect on the expression of its own transcription factor, HIF-1, and on VEGF itself PMID: 18305118
  34. By regulating VEGFR2 expression and activation, PKC-epsilon expression is critical for activation of Akt and eNOS by VEGF and contributes to VEGF-stimulated Erk activation, whereas PKC-alpha has opposite effects. PMID: 18323518
  35. Angiopoietin-2 may support the angiogenic effects of VEGF-A in these corpus luteum stages. PMID: 18541291
  36. DNA methylation of VEGF in blg(+/-) was noticeably decreased. PMID: 18789616
  37. VEGF-dependent production of NO by eNOS is mediated by as cascade involving ezrin, calpain, PI-3-kinase, AMPK and AKT. PMID: 19038867
  38. ALK1 and ALK5 are both essential for correct regulation of VEGF, and that disruption of either pathway leads to disease. PMID: 19506300
  39. inhibition of NO production by Ang-1, via phosphorylation of eNOS on Thr(497) by PKC zeta, is responsible, at least in part, for inhibition of VEGF-stimulated endothelial permeability by Ang-1. PMID: 19564638

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Subcellular Location
Protein Families
PDGF/VEGF growth factor family
Database Links
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