Mouse monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA kit

Code CSB-E07430m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


The mouse MCP-1 ELISA kit uses the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse MCP-1 in the serum, plasma, or tissue homogenates. Antibody specific for MCP-1 has been pre-coated onto the microplate. Standards and samples are pipetted into the wells and any MCP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated MCP-1 antibody is added to the wells. After washing, avidin conjugated HRP is added to the wells, forming an antibody-antigen-enzyme-labeled antibody complex. Following a wash to remove any unbound HRP-avidin, the TMB substrate solution is added to the wells, and the color develops into blue. The color changes from blue to yellow after adding the stop solution to the wells. The color intensity is proportional to the amount of MCP-1 bound in the initial step.

CCL2, also known as MCP-1, is a monocyte chemoattractant protein well-known for its ability to drive the chemotaxis of myeloid and lymphoid cells. CCL2 is found in the circulation and has been identified as a diagnostic biomarker of breast cancer and prostate cancer. In tissues, CCL2 attracts leukocytes to sites of infection or injury to mediate defense and repair. The CCL2-CCR2 signaling initiates several intracellular downstream signaling pathways, including JAK2/STAT3, MAPK, and PI3K pathways that are involved in tumor progression, such as increasing tumor cell proliferation and invasiveness and creating a tumor microenvironment through increased angiogenesis and recruitment of immunosuppressive cells. CCL2 is involved in the pathogenesis of inflammatory and neurodegenerative diseases, such as atherosclerosis, diabetes, rheumatoid arthritis (RA), neuropathic pain, and multiple sclerosis.

Target Name chemokine (C-C motif) ligand 2
Alternative Names Ccl2 ELISA Kit; Je ELISA Kit; Mcp1 ELISA Kit; Scya2 ELISA Kit; C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Platelet-derived growth factor-inducible protein JE ELISA Kit; Small-inducible cytokine A2 ELISA Kit
Abbreviation CCL2
Uniprot No. P10148
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %88
Range %82-94
1:2Average %95
Range %91-98
1:4Average %102
Range %96-108
1:8Average %95
Range %89-98
The recovery of mouse MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8882-94
EDTA plasma (n=4)9792-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
50001.946 1.864 1.905 1.806
25001.294 1.414 1.354 1.255
12500.861 0.843 0.852 0.753
6250.555 0.546 0.551 0.452
3120.347 0.356 0.352 0.253
1560.267 0.258 0.263 0.164
780.171 0.179 0.175 0.076
00.099 0.098 0.099  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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Is it possible to test the #CSB-E07430m product with urine?

Thanks for your inquiry. Usually we suggest you test the sample types mentioned in the kit manual. If you want to test urine sample, you can do a pretest first.

Target Background

(From Uniprot)
Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
Gene References into Functions
  1. Targeting a reduction of MCP-1 opens the door to a better understanding of the mechanistic consequences of ceramide accumulation. PMID: 29379059
  2. CCL2 played important roles in regulating platelet function and arterial thrombosis through the PKCalpha-P38MAPK-HSP27 pathway. PMID: 29864522
  3. these results suggested that the IL-1beta-induced recruitment of monocyte/macrophage lineage cells to inflamed synovial membranes in TMJ was further augmented by the cell-cell interaction-induced secretion of MCP-1 from the inflammation site, possibly resulting in prolonged inflammatory responses in TMJ synovial tissue. PMID: 29535277
  4. NOX4 is induced in early alcoholic liver injury and regulates CCR2/CCL2 mRNA stability thereby promoting recruitment of inflammatory cells and production of proinflammatory cytokines. PMID: 28383062
  5. Ccl2-Ccr2 signaling recruits a distinct fetal microchimeric population that rescues delayed maternal wound healing. PMID: 28516946
  6. itration reduced the potential of CCL2 to stimulate monocyte migration in diffusion gradient chemotaxis assays (p < 0.05). This was consistent with a trend towards reduced affinity of the nitrated chemokine for its cognate receptor CCR2b. PMID: 28290520
  7. CCL2 enables the prolonged Mesenchymal stem cells-T cell interactions needed for sufficient suppression of autoreactive T cells PMID: 28117437
  8. The data highlight Klf4 as an essential MCPIP1-dependent modulator of innate immunity that protects against excessive and self-destructive inflammation. PMID: 29975947
  9. a novel mechanism that contributes to the EMT and metastatic phenotype observed in a subset of ADT-resistant prostate cancer, where the CCL2 is stimulated through the inactivated of AR-mediated SPDEF. PMID: 29477409
  10. Macrophage subtypes enhanced the osteogenesis in transwell setting and the transition from M1 to M2 was associated with an increase in bone anabolic factors CCL2/MCP-1, CCL5/RANTES and IGF-1 in vitro. PMID: 28782174
  11. Axotomy Leads to Reduced Calcium Increase and Earlier Termination of CCL2 Release in Spinal Motoneurons. PMID: 28017131
  12. Our results showed that MIF regulates MCP-1 expression in hepatocytes of injured liver via CD74, CD44, and p38 MAPK in an autocrine manner. PMID: 27273604
  13. these studies identify a novel role for the microbiota in shaping immunity, which includes induction of CCL2 levels that control homeostatic trafficking of plasmacytoid dendritic cells PMID: 27827374
  14. These studies show that elevated levels of CCL2 in the CNS through increased astrocyte expression can significantly interact with the behavioral effects of alcohol. PMID: 28431906
  15. Topical application of glycolic acid suppresses the UVB induced IL-6, IL-8, MCP-1 and COX-2 inflammation by modulating NF-kappaB signaling pathway in mouse skin. PMID: 28330776
  16. Study demonstrated that CD36 deletion regulated MCP-1 expression at the transcriptional level by changing the acetylation of histones binding to the MCP-1 promoters in hepatocytes, promoting macrophage migration to the liver, and aggravating hepatic inflammatory response and fibrosis. PMID: 27967209
  17. Despite higher caloric intake and elevated body weights, carbohydrate restriction lowered serum MCP-1 levels, reduced prostate macrophage infiltration, reduced prostate weight, but failed to slow adenocarcinoma development. PMID: 28244492
  18. Data indicate that ASC-J9(R) can suppress CD4+ T cell migration via decreasing the cytokine CCL2 in vitro and in vivo. PMID: 27564257
  19. PDAC responds to radiotherapy by producing CCL2, which recruits Ly6C(+)CCR2(+) monocytes to support tumor proliferation and neovascularization after radiotherapy. PMID: 27354473
  20. Chemokine receptor 2 (CCR2(+)) monocytes invade the hippocampus between 1 and 3 d after SE. In contrast, only an occasional CD3(+) T lymphocyte was encountered 3 d after SE. The initial cellular sources of the chemokine CCL2, a ligand for CCR2, included perivascular macrophages and microglia. The induction of the proinflammatory cytokine IL-1beta was greater in FACS-isolated microglia than in brain-invading monocytes PMID: 27601660
  21. data suggest that MCP-1 may be a necessary component of the inflammatory response required for adipose tissue protection, remodeling and healthy expansion in the FVB/N strain in response to high-fat diet feedings PMID: 26620890
  22. MCP-1 knockout mice exposed for voluntary wheel running displayed changes in brain cytokine expression and glial activation. PMID: 28088610
  23. Carvedilol treatment also significantly reduced the levels of proinflammatory cytokines tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-6, transforming growth factor (TGF)-beta1 and monocyte chemoattractant protein (MCP)-1 in the lung tissue. PMID: 27619518
  24. High dilutions of antimony modulate cytokines production and macrophage - Leishmania (L.) amazonensis interaction in vitro.( PMID: 28092793
  25. Both IL-6 protein production and transcript levels were downregulated by RA in respiratory tract epithelial cells (LETs) , but upregulated in macrophages (MACs). RA also increased transcript levels of MCP-1, GMCSF, and IL-10 in MACs, but not in LETs. Conversely, when LETs, but not MACs, were exposed to RA PMID: 27940088
  26. FSAP deficiency causes an increase in CCL2 expression and CCL2-mediated infiltration of leukocytes into the injured vessel PMID: 27431088
  27. miR-33 suppresses CCL2 expression via targeting at the 3'UTR. The miR-33/CCL2 axis in chondrocytes regulates the monocyte chemotaxis. PMID: 27129293
  28. Sca-1(+) progenitor cells found to exhibit increased migration was associated with elevated levels of chemokines, CCL2 (chemokine (C-C motif) ligand 2) and CXCL1 (chemokine (C-X-C motif) ligand 1), and their corresponding receptors. PMID: 27300479
  29. that MCP-1 inhibition restores glomerular endothelial glycocalyx and barrier function and reduces tissue inflammation in the presence of ongoing diabetic injury PMID: 28837800
  30. In LLC-bearing mice, the high-fat diet increased and MCP-1 deficiency decreased plasma TRAP 5b; neither the high-fat diet nor MCP-1 deficiency resulted in significant changes in plasma concentration of osteocalcin. PMID: 28177896
  31. Grooved surfaces showed time-dependent increase in soluble mediators involved in cell fusion, CCL2 and MMP-9 PMID: 27102570
  32. In this study, the effect of continuous IL-4 delivery or bioactive implant coating that constitutively releases a protein inhibitor of CCL2 signaling (7ND) on particle induced osteolysis were studied in the murine continuous femoral intramedullary particle infusion model PMID: 27114284
  33. Blockade of MCP-1 activity significantly reduced inflammatory pleural effusion formation in a CAR model PMID: 27878909
  34. CCL2 recruits T cells into the brain in a CCR2-independent manner. PMID: 28836736
  35. Expression of MCP-1 induced by IL-17 requires NF-kappaB through the phosphorylation of p65 in cardiac myocytes. PMID: 28593659
  36. this study demonstrates that killed probiotics can reduce viral disease severity and identify that the MCP-1 pathway might act as a key mediator in the improved antiviral immune response PMID: 27072606
  37. tumor-promoting role for CCL2 acting through CCR2 on the tumor microenvironment PMID: 27820834
  38. type I interferons, besides their known antiviral properties, can initiate the recruitment and activation of leukocytes via induction of chemokine expression including CCL2. PMID: 26992431
  39. In cultured macrophages, LPS-induced expression of monocyte chemoattractant protein (MCP)-1 was suppressed by NaN3 through inhibition of STAT1 and IkappaBzeta activities. PMID: 28391993
  40. study concludes that CCL2 signaling onto melanin-concentrating hormone neurons represents a core mechanism that relays peripheral inflammation to sickness behavior PMID: 27733491
  41. The increased migratory phenotypes are dependent on secreted factors, including the cytokine interleukin-6 and the chemokine CCL2. We propose that Delta122p53 (and Delta133p53) acts in a similar manner to 'gain-of-function' mutant p53 proteins to promote migration, invasion and metastasis, which may contribute to poor survival in patients with Delta133p53-expressing tumours PMID: 26996665
  42. CCL5 paradoxically limits macrophage accumulation in the injured kidney during renin angiotensin system (RAS) activation by constraining the proinflammatory actions of CCL2. PMID: 27640148
  43. This study establishes ECL1i as the first allosteric inhibitor of CCR2 with functional selectivity. PMID: 26979087
  44. Constitutive expression of CCL2 by the mouse mammary epithelium induces a state of low level chronic inflammation that increases stromal density and elevates cancer risk. We propose that CCL2-driven inflammation contributes to the increased risk of breast cancer observed in women with high mammographic density. PMID: 28077158
  45. the individual and combined actions of CCL2/CCR2 and CX3CL1/CX3CR1 in hypoxia-induced pulmonary hypertension in mice, is reported. PMID: 28125278
  46. CCL2 acting at the suprachiasmatic nuclei level is important for the circadian effects of immune activation. PMID: 27178133
  47. FAP-STAT3-CCL2 signaling in Cancer-associated fibroblasts (CAF) was sufficient to program an inflammatory component of the tumor microenvironment, which may have particular significance in desmoplasia-associated cancers. PMID: 27216177
  48. Macrophages induce neural stem cells migrating into the spinal cord injury site possibly through promoting the secretion of MCP-1. PMID: 27609570
  49. this study shows that CCL2 supports the classical activation of macrophages, with miR-9 mediated down-regulation of Dusp6 and enhanced ERK-mediated signal transduction possibly mediating this enhanced pro-inflammatory gene expression PMID: 28242024
  50. macrophage-specific chemokine (C-C Motif) receptor 2 (Ccr2) knockout (M-Ccr2KO) and intestinal epithelial cell-specific tamoxifen-inducible Ccl2 knockout (Vil-Ccl2KO) mice, were studied. PMID: 27508875

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Subcellular Location Secreted.
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: mmu:20296

STRING: 10090.ENSMUSP00000000193

UniGene: Mm.290320

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