Mouse monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA kit

Instructions
Code CSB-E07430m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name chemokine (C-C motif) ligand 2
Alternative Names Ccl2 ELISA Kit; Je ELISA Kit; Mcp1 ELISA Kit; Scya2 ELISA Kit; C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Platelet-derived growth factor-inducible protein JE ELISA Kit; Small-inducible cytokine A2 ELISA Kit
Abbreviation CCL2
Uniprot No. P10148
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %82-94
1:2Average %95
Range %91-98
1:4Average %102
Range %96-108
1:8Average %95
Range %89-98
Recovery
The recovery of mouse MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8882-94
EDTA plasma (n=4)9792-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50001.946 1.864 1.905 1.806
25001.294 1.414 1.354 1.255
12500.861 0.843 0.852 0.753
6250.555 0.546 0.551 0.452
3120.347 0.356 0.352 0.253
1560.267 0.258 0.263 0.164
780.171 0.179 0.175 0.076
00.099 0.098 0.099  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Q&A and Customer Reviews

 Q&A
Q:

Is it possible to test the #CSB-E07430m product with urine?

A:
Thanks for your inquiry. Usually we suggest you test the sample types mentioned in the kit manual. If you want to test urine sample, you can do a pretest first.

Target Data

Function Chemotactic factor that attracts monocytes, but not neutrophils.
Gene References into Functions
  1. Targeting a reduction of MCP-1 opens the door to a better understanding of the mechanistic consequences of ceramide accumulation. PMID: 29379059
  2. CCL2 played important roles in regulating platelet function and arterial thrombosis through the PKCalpha-P38MAPK-HSP27 pathway. PMID: 29864522
  3. these results suggested that the IL-1beta-induced recruitment of monocyte/macrophage lineage cells to inflamed synovial membranes in TMJ was further augmented by the cell-cell interaction-induced secretion of MCP-1 from the inflammation site, possibly resulting in prolonged inflammatory responses in TMJ synovial tissue. PMID: 29535277
  4. NOX4 is induced in early alcoholic liver injury and regulates CCR2/CCL2 mRNA stability thereby promoting recruitment of inflammatory cells and production of proinflammatory cytokines. PMID: 28383062
  5. Ccl2-Ccr2 signaling recruits a distinct fetal microchimeric population that rescues delayed maternal wound healing. PMID: 28516946
  6. itration reduced the potential of CCL2 to stimulate monocyte migration in diffusion gradient chemotaxis assays (p < 0.05). This was consistent with a trend towards reduced affinity of the nitrated chemokine for its cognate receptor CCR2b. PMID: 28290520
  7. CCL2 enables the prolonged Mesenchymal stem cells-T cell interactions needed for sufficient suppression of autoreactive T cells PMID: 28117437
  8. The data highlight Klf4 as an essential MCPIP1-dependent modulator of innate immunity that protects against excessive and self-destructive inflammation. PMID: 29975947
  9. a novel mechanism that contributes to the EMT and metastatic phenotype observed in a subset of ADT-resistant prostate cancer, where the CCL2 is stimulated through the inactivated of AR-mediated SPDEF. PMID: 29477409
  10. Macrophage subtypes enhanced the osteogenesis in transwell setting and the transition from M1 to M2 was associated with an increase in bone anabolic factors CCL2/MCP-1, CCL5/RANTES and IGF-1 in vitro. PMID: 28782174
  11. Axotomy Leads to Reduced Calcium Increase and Earlier Termination of CCL2 Release in Spinal Motoneurons. PMID: 28017131
  12. Our results showed that MIF regulates MCP-1 expression in hepatocytes of injured liver via CD74, CD44, and p38 MAPK in an autocrine manner. PMID: 27273604
  13. these studies identify a novel role for the microbiota in shaping immunity, which includes induction of CCL2 levels that control homeostatic trafficking of plasmacytoid dendritic cells PMID: 27827374
  14. These studies show that elevated levels of CCL2 in the CNS through increased astrocyte expression can significantly interact with the behavioral effects of alcohol. PMID: 28431906
  15. Topical application of glycolic acid suppresses the UVB induced IL-6, IL-8, MCP-1 and COX-2 inflammation by modulating NF-kappaB signaling pathway in mouse skin. PMID: 28330776
  16. Study demonstrated that CD36 deletion regulated MCP-1 expression at the transcriptional level by changing the acetylation of histones binding to the MCP-1 promoters in hepatocytes, promoting macrophage migration to the liver, and aggravating hepatic inflammatory response and fibrosis. PMID: 27967209
  17. Despite higher caloric intake and elevated body weights, carbohydrate restriction lowered serum MCP-1 levels, reduced prostate macrophage infiltration, reduced prostate weight, but failed to slow adenocarcinoma development. PMID: 28244492
  18. Data indicate that ASC-J9(R) can suppress CD4+ T cell migration via decreasing the cytokine CCL2 in vitro and in vivo. PMID: 27564257
  19. PDAC responds to radiotherapy by producing CCL2, which recruits Ly6C(+)CCR2(+) monocytes to support tumor proliferation and neovascularization after radiotherapy. PMID: 27354473
  20. Chemokine receptor 2 (CCR2(+)) monocytes invade the hippocampus between 1 and 3 d after SE. In contrast, only an occasional CD3(+) T lymphocyte was encountered 3 d after SE. The initial cellular sources of the chemokine CCL2, a ligand for CCR2, included perivascular macrophages and microglia. The induction of the proinflammatory cytokine IL-1beta was greater in FACS-isolated microglia than in brain-invading monocytes PMID: 27601660
  21. data suggest that MCP-1 may be a necessary component of the inflammatory response required for adipose tissue protection, remodeling and healthy expansion in the FVB/N strain in response to high-fat diet feedings PMID: 26620890
  22. MCP-1 knockout mice exposed for voluntary wheel running displayed changes in brain cytokine expression and glial activation. PMID: 28088610
  23. Carvedilol treatment also significantly reduced the levels of proinflammatory cytokines tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-6, transforming growth factor (TGF)-beta1 and monocyte chemoattractant protein (MCP)-1 in the lung tissue. PMID: 27619518
  24. High dilutions of antimony modulate cytokines production and macrophage - Leishmania (L.) amazonensis interaction in vitro.( PMID: 28092793
  25. Both IL-6 protein production and transcript levels were downregulated by RA in respiratory tract epithelial cells (LETs) , but upregulated in macrophages (MACs). RA also increased transcript levels of MCP-1, GMCSF, and IL-10 in MACs, but not in LETs. Conversely, when LETs, but not MACs, were exposed to RA PMID: 27940088
  26. FSAP deficiency causes an increase in CCL2 expression and CCL2-mediated infiltration of leukocytes into the injured vessel PMID: 27431088
  27. miR-33 suppresses CCL2 expression via targeting at the 3'UTR. The miR-33/CCL2 axis in chondrocytes regulates the monocyte chemotaxis. PMID: 27129293
  28. Sca-1(+) progenitor cells found to exhibit increased migration was associated with elevated levels of chemokines, CCL2 (chemokine (C-C motif) ligand 2) and CXCL1 (chemokine (C-X-C motif) ligand 1), and their corresponding receptors. PMID: 27300479
  29. that MCP-1 inhibition restores glomerular endothelial glycocalyx and barrier function and reduces tissue inflammation in the presence of ongoing diabetic injury PMID: 28837800
  30. In LLC-bearing mice, the high-fat diet increased and MCP-1 deficiency decreased plasma TRAP 5b; neither the high-fat diet nor MCP-1 deficiency resulted in significant changes in plasma concentration of osteocalcin. PMID: 28177896
  31. Grooved surfaces showed time-dependent increase in soluble mediators involved in cell fusion, CCL2 and MMP-9 PMID: 27102570
  32. In this study, the effect of continuous IL-4 delivery or bioactive implant coating that constitutively releases a protein inhibitor of CCL2 signaling (7ND) on particle induced osteolysis were studied in the murine continuous femoral intramedullary particle infusion model PMID: 27114284
  33. Blockade of MCP-1 activity significantly reduced inflammatory pleural effusion formation in a CAR model PMID: 27878909
  34. CCL2 recruits T cells into the brain in a CCR2-independent manner. PMID: 28836736
  35. Expression of MCP-1 induced by IL-17 requires NF-kappaB through the phosphorylation of p65 in cardiac myocytes. PMID: 28593659
  36. this study demonstrates that killed probiotics can reduce viral disease severity and identify that the MCP-1 pathway might act as a key mediator in the improved antiviral immune response PMID: 27072606
  37. tumor-promoting role for CCL2 acting through CCR2 on the tumor microenvironment PMID: 27820834
  38. type I interferons, besides their known antiviral properties, can initiate the recruitment and activation of leukocytes via induction of chemokine expression including CCL2. PMID: 26992431
  39. In cultured macrophages, LPS-induced expression of monocyte chemoattractant protein (MCP)-1 was suppressed by NaN3 through inhibition of STAT1 and IkappaBzeta activities. PMID: 28391993
  40. study concludes that CCL2 signaling onto melanin-concentrating hormone neurons represents a core mechanism that relays peripheral inflammation to sickness behavior PMID: 27733491
  41. The increased migratory phenotypes are dependent on secreted factors, including the cytokine interleukin-6 and the chemokine CCL2. We propose that Delta122p53 (and Delta133p53) acts in a similar manner to 'gain-of-function' mutant p53 proteins to promote migration, invasion and metastasis, which may contribute to poor survival in patients with Delta133p53-expressing tumours PMID: 26996665
  42. CCL5 paradoxically limits macrophage accumulation in the injured kidney during renin angiotensin system (RAS) activation by constraining the proinflammatory actions of CCL2. PMID: 27640148
  43. This study establishes ECL1i as the first allosteric inhibitor of CCR2 with functional selectivity. PMID: 26979087
  44. Constitutive expression of CCL2 by the mouse mammary epithelium induces a state of low level chronic inflammation that increases stromal density and elevates cancer risk. We propose that CCL2-driven inflammation contributes to the increased risk of breast cancer observed in women with high mammographic density. PMID: 28077158
  45. the individual and combined actions of CCL2/CCR2 and CX3CL1/CX3CR1 in hypoxia-induced pulmonary hypertension in mice, is reported. PMID: 28125278
  46. CCL2 acting at the suprachiasmatic nuclei level is important for the circadian effects of immune activation. PMID: 27178133
  47. FAP-STAT3-CCL2 signaling in Cancer-associated fibroblasts (CAF) was sufficient to program an inflammatory component of the tumor microenvironment, which may have particular significance in desmoplasia-associated cancers. PMID: 27216177
  48. Macrophages induce neural stem cells migrating into the spinal cord injury site possibly through promoting the secretion of MCP-1. PMID: 27609570
  49. this study shows that CCL2 supports the classical activation of macrophages, with miR-9 mediated down-regulation of Dusp6 and enhanced ERK-mediated signal transduction possibly mediating this enhanced pro-inflammatory gene expression PMID: 28242024
  50. macrophage-specific chemokine (C-C Motif) receptor 2 (Ccr2) knockout (M-Ccr2KO) and intestinal epithelial cell-specific tamoxifen-inducible Ccl2 knockout (Vil-Ccl2KO) mice, were studied. PMID: 27508875
  51. PERK and IRE1alpha/XBP1 in the unfolded protein response differentially regulate the expression of CXCL10 and CCL2 likely through modulation of ER stress-induced NF-kappaB RelA and STAT3 activation. PMID: 28065589
  52. microRNA-155 promoted the directional migration of smooth muscle progenitor cells from the adventitia by regulating the monocyte chemoattractant protein 1 concentration gradient, which aggravated transplant arteriosclerosis. PMID: 27079875
  53. Kupffer cells secrete CCL2 to promote CCR2-expressing neutrophil recruitment from the bone marrow and subsequent infiltration into the liver during ischemia/reperfusion injury. These findings reveal a novel pro-inflammatory role of cell-mediated CCL2-CCR2 interactions during this sterile insult. PMID: 27229110
  54. IL-6 Mediates Macrophage Infiltration after Irradiation via Up-regulation of CCL2/CCL5 in Non-small Cell Lung Cancer PMID: 28054838
  55. These findings suggested that androgen-AR suppressed obesity-induced chronic inflammation in adipose tissue. PMID: 27392713
  56. this study shows that CCL2 ablation provides highly increased susceptibility to Japanese encephalitis virus PMID: 27260136
  57. Data show that loss of loss of matrix metalloproteinase-3 (MMP-3) repressed the upregulation of the chemokines monocyte chemoattractant protein (MCP)-1 and (C-X-C motif) ligand 1 (CXCL1). PMID: 27809288
  58. These data indicated that alpha7-nAChR caused the inhibition of ASPinduced activation of p38 kinase and NFkappa B to inhibit the production of MCP1 and keratinocytederived chemokine. PMID: 27572255
  59. Female MRL/lpr mice treated with dual blockade of the chemokines CCL2 and CXCL12 showed significantly more effective than either monotherapy in preventing proteinuria and immune complex glomerulonephritis. PMID: 27392463
  60. Data show that RP105 knockout (-/-) smooth muscle cells and mast cells secreted higher levels of chemokine (C-C motif) ligand 2 protein (CCL2). PMID: 27053419
  61. CCL2/CCL3 double-mutant animals are viable, fertile, and do not present with gross abnormalities. Cuprizone increased CCL2 expression in wild-type but not mutant miCuprizone-induced demyelination, oligodendrocyte loss, and astrogliosis were significantly ameliorated in the cortex but not corpus callosum of chemokine-deficient animals. PMID: 25663168
  62. study suggests that MCP-1 might promote intracerebral hemorrhage-induced microglia activation and toxicity to neurons PMID: 26851629
  63. Results show that CCL2 from astrocytes selectively induces microglial activation associated with M1 polarization and enhanced migration ability. PMID: 26910882
  64. kiss1 and kiss1r mRNA levels were significantly higher in old compared to reproductive-aged mice, and diet-induced obesity did not alter kiss1 or kiss1r mRNA levels. Compared to young control mice, young MCP-1 knockout mice had significantly lower ovarian kiss1 mRNA PMID: 26879207
  65. In 3T3-L1 preadipocytes, Ad36, but not E4orf1, increased MCP1 and TNFalpha mRNA, in presence of LPS stimulation. PMID: 25450249
  66. Probiotic Lactobacillus reuteri can prevent the exacerbating effects of stressor exposure on Citrobacter-induced colitis; one mechanism by which this occurs is through downregulation of the chemokine CCL2. PMID: 26422754
  67. CCL2 and its receptor CCR2 are important for the formation of osteoclasts and foreign body giant cells. PMID: 26205994
  68. FAMP significantly preserved cytochrome C in the mitochondrial fraction and inhibited its release into the cytosolic fraction in the heart, but did not significantly reduce mRNA levels of monocyte chemoattractant protein-1 PMID: 26476037
  69. Mycobacterium chelonae activates the gene expressions of chemokine (C-C motif) ligand 2 (CCL2) and CCL5 in murine bone marrow-derived macrophages and in vivo mouse model. PMID: 26626357
  70. This study demonstrated that Chemokine CCL2 Mediates the Seizure-enhancing Effects of Systemic Inflammation. PMID: 27030762
  71. cl2 mRNAs increased within 5 h after injury in mouse cortical slices. PMID: 25895671
  72. Data show that cathepsins S (CatS) regulates CCL2 chemokine expression by modulation of CD74 antigen processing. PMID: 26358505
  73. Data show that monocyte chemotactic protein 1 (MCP-1) expressed by HEK293T cells could apparently increase the migration of macrophages. PMID: 26927370
  74. Data indicate that Zoledronic acid (ZA) decreased mesenchymal stem cells (MSC)-monocyte chemotactic protein 1 (MCP-1) and therefore reduced the recruitment of tumor-associated macrophages (TAMs) to the tumor sites and hence inhibited the tumor growth. PMID: 26305552
  75. activation of canonical Wnt signaling might contribute to the focal retinal degeneration of mouse models with Ccl2 and Cx3cr1 deficiency PMID: 26476672
  76. Genetically modified mice overexpressing a human PS1 familial Alzheimer's disease mutant on a CCL2 null background significantly decreased hippocampal neurogenesis and affected age-dependent learning and memory. PMID: 26112421
  77. Given the important role of the TAM phenotype in the tumor microenvironment, inhibitory effect of luteolin on the monocyte recruitment and cancer migration via suppression of the TAM-secreted CCL2 suggests a novel therapeutic approach to treat tumors. PMID: 26766793
  78. In 3T3-L1 adipocytes, catechin and quercetin attenuated TNF-alpha-induced elevated protein carbonyls, increased proinflammatory cytokine expression (MCP-1, resistin), and decreased adiponectin. PMID: 25620282
  79. FXR down regulates MCP-1 expression in macrophages. PMID: 26474702
  80. the present study shows that sildenafil modulates inflammation, with the involvement of MMP-9, MCP-1, and CCR-2, and also contributes to myelin repair. PMID: 26515692
  81. CCL2 overexpression is sufficient to induce macrophage accumulation in uninjured L5 DRGs and increase the regenerative capacity of DRG neurons via a STAT3-dependent mechanism. PMID: 26431741
  82. tests the hypothesis that deletion of TRPV1 exaggerates salt-sensitive hypertension-induced renal injury due to enhanced inflammatory responses via monocyte chemoattractant protein-1 (MCP-1)/C-C chemokine receptor 2 (CCR2)-dependent pathways.\ PMID: 25585624
  83. CCL2 regulated T cell suppression of Polymorphonuclear Myeloid-myeloid-derived suppressor cells in a STAT3-mediated manner. PMID: 26146082
  84. The results of this study suggested that CCL2-mediated neuron-macrophage interaction plays a critical role for amplification and maintenance of enhanced regenerative capacity by preconditioning peripheral nerve injury. PMID: 26631474
  85. Results show that nerve-released CCL2 supports prostate cancer migration and perineural invasion though CCR2-mediated signaling. PMID: 25312961
  86. Data show that monocyte accumulation in the brain is dependent on chemokines CCL2 and CCL7. PMID: 26401006
  87. prenatal immunological stress delays the GnRH neuron migration in the nasal compartment of mouse fetuses, which may be mediated by the regulation of IL-6, MCP-1 and LIF secretion in the maternal-fetal system. PMID: 25341493
  88. These results indicate that MEK inhibitor diminishes Nasopharyngeal carcinoma cell proliferation and NPC-induced osteoclastogenesis via modulating CCL2 and CXCL16 expressions. PMID: 26058873
  89. Telmisartan treatment targets inflammatory cytokines to suppress the pathogenesis of acute colitis induced by dextran sulphate sodium. PMID: 25873126
  90. Inactivation of Ccl2 in an Lkb1-driven mouse model of endometrial cancer slowed tumor progression and increased survival. PMID: 26413869
  91. Obesity triggers enhanced MDSC accumulation in murine renal tumors via elevated local production of CCL2 PMID: 25769110
  92. MCP-1 protects the kidney during the acute inflammatory response following renal I/R injury. PMID: 25875776
  93. MCP-1 promotes the proliferation and invasion of osteosarcoma cells and upregulates the expression of AKT. PMID: 25695619
  94. The results indicate that testosterone enhances LPS-induced IL-6 and MCP-1 expression by activating the ERK1/2/NF-kappaB signalling pathways in 3T3-L1 adipocytes. PMID: 25738264
  95. that mast cells, recruited towards CCL2 and CCL5 expressed by epithelium induced to proliferate by Human Papillomavirus 16 E7 protein PMID: 25340820
  96. These results have suggested that the activation of MyD88 pathway by constitutive low-level IL-1beta expression is essential for pressure force-induced CXCL2 and CCL2 expression in osteoblasts. PMID: 25603464
  97. Stromal cell-derived CCL2 (in contrast to CCL2 immune cell-derived) tightly controls monocyte/macrophage recruitment into the lesion and plays a major role in the development of neuropathic pain. PMID: 25449579
  98. cholesterol did not cause increased the expression of CD14 in the aforementioned cells, and the addition of LPS to cholesterol-treated monocytic cells did not result in enhanced the expression of CCL2. PMID: 25497142
  99. aromatase may play a key role in the pathogenesis of Sjogren syndrome-like lesions by controlling the target organ and adipose tissue-associated macrophage; increased MPC1 protein expression has a role in adiposity PMID: 25447050
  100. data implicate MCP-1 as the key molecule governing tPA-induced fluid accumulation. The role of MCP-1 in the development of other exudative effusions warrants examination. PMID: 25474480

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Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: mmu:20296

STRING: 10090.ENSMUSP00000000193

UniGene: Mm.290320

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