Mouse monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA kit

Instructions
Code CSB-E07430m
Size 96T,5×96T,10×96T

Product Details

Target Name chemokine (C-C motif) ligand 2
Alternative Names GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF, monocyte chemoattractant protein-1|monocyte chemotactic and activating factor|monocyte secretory protein JE|small inducible cytokine
Abbreviation CCL2
Uniprot No. P10148
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %82-94
1:2Average %95
Range %91-98
1:4Average %102
Range %96-108
1:8Average %95
Range %89-98
Recovery
The recovery of mouse MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8882-94
EDTA plasma (n=4)9792-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50001.946 1.864 1.905 1.806
25001.294 1.414 1.354 1.255
12500.861 0.843 0.852 0.753
6250.555 0.546 0.551 0.452
3120.347 0.356 0.352 0.253
1560.267 0.258 0.263 0.164
780.171 0.179 0.175 0.076
00.099 0.098 0.099  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Q&A and Customer Reviews

 Q&A
Q:

Is it possible to test the #CSB-E07430m product with urine?

A:
Thanks for your inquiry. Usually we suggest you test the sample types mentioned in the kit manual. If you want to test urine sample, you can do a pretest first.
 Customer Reviews

Target Data

Function Chemotactic factor that attracts monocytes, but not neutrophils.
Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: mmu:20296

STRING: 10090.ENSMUSP00000000193

UniGene: Mm.290320

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