Ccl2 Antibody

Code CSB-PA004783ZA01MO
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Product Details

Full Product Name Rabbit anti-Mus musculus (Mouse) CCL2 Polyclonal antibody
Uniprot No. P10148
Target Names CCL2
Alternative Names Ccl2 antibody; Je antibody; Mcp1 antibody; Scya2 antibody; C-C motif chemokine 2 antibody; Monocyte chemoattractant protein 1 antibody; Monocyte chemotactic protein 1 antibody; MCP-1 antibody; Platelet-derived growth factor-inducible protein JE antibody; Small-inducible cytokine A2 antibody
Raised in Rabbit
Species Reactivity Mus musculus
Immunogen Recombinant Mus musculus Ccl2 protein
Immunogen Species Mus musculus (Mouse)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (12-14 weeks)

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Target Background

Function
(From Uniprot)
Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
Gene References into Functions
  1. Targeting a reduction of MCP-1 opens the door to a better understanding of the mechanistic consequences of ceramide accumulation. PMID: 29379059
  2. CCL2 played important roles in regulating platelet function and arterial thrombosis through the PKCalpha-P38MAPK-HSP27 pathway. PMID: 29864522
  3. these results suggested that the IL-1beta-induced recruitment of monocyte/macrophage lineage cells to inflamed synovial membranes in TMJ was further augmented by the cell-cell interaction-induced secretion of MCP-1 from the inflammation site, possibly resulting in prolonged inflammatory responses in TMJ synovial tissue. PMID: 29535277
  4. NOX4 is induced in early alcoholic liver injury and regulates CCR2/CCL2 mRNA stability thereby promoting recruitment of inflammatory cells and production of proinflammatory cytokines. PMID: 28383062
  5. Ccl2-Ccr2 signaling recruits a distinct fetal microchimeric population that rescues delayed maternal wound healing. PMID: 28516946
  6. itration reduced the potential of CCL2 to stimulate monocyte migration in diffusion gradient chemotaxis assays (p < 0.05). This was consistent with a trend towards reduced affinity of the nitrated chemokine for its cognate receptor CCR2b. PMID: 28290520
  7. CCL2 enables the prolonged Mesenchymal stem cells-T cell interactions needed for sufficient suppression of autoreactive T cells PMID: 28117437
  8. The data highlight Klf4 as an essential MCPIP1-dependent modulator of innate immunity that protects against excessive and self-destructive inflammation. PMID: 29975947
  9. a novel mechanism that contributes to the EMT and metastatic phenotype observed in a subset of ADT-resistant prostate cancer, where the CCL2 is stimulated through the inactivated of AR-mediated SPDEF. PMID: 29477409
  10. Macrophage subtypes enhanced the osteogenesis in transwell setting and the transition from M1 to M2 was associated with an increase in bone anabolic factors CCL2/MCP-1, CCL5/RANTES and IGF-1 in vitro. PMID: 28782174
  11. Axotomy Leads to Reduced Calcium Increase and Earlier Termination of CCL2 Release in Spinal Motoneurons. PMID: 28017131
  12. Our results showed that MIF regulates MCP-1 expression in hepatocytes of injured liver via CD74, CD44, and p38 MAPK in an autocrine manner. PMID: 27273604
  13. these studies identify a novel role for the microbiota in shaping immunity, which includes induction of CCL2 levels that control homeostatic trafficking of plasmacytoid dendritic cells PMID: 27827374
  14. These studies show that elevated levels of CCL2 in the CNS through increased astrocyte expression can significantly interact with the behavioral effects of alcohol. PMID: 28431906
  15. Topical application of glycolic acid suppresses the UVB induced IL-6, IL-8, MCP-1 and COX-2 inflammation by modulating NF-kappaB signaling pathway in mouse skin. PMID: 28330776
  16. Study demonstrated that CD36 deletion regulated MCP-1 expression at the transcriptional level by changing the acetylation of histones binding to the MCP-1 promoters in hepatocytes, promoting macrophage migration to the liver, and aggravating hepatic inflammatory response and fibrosis. PMID: 27967209
  17. Despite higher caloric intake and elevated body weights, carbohydrate restriction lowered serum MCP-1 levels, reduced prostate macrophage infiltration, reduced prostate weight, but failed to slow adenocarcinoma development. PMID: 28244492
  18. Data indicate that ASC-J9(R) can suppress CD4+ T cell migration via decreasing the cytokine CCL2 in vitro and in vivo. PMID: 27564257
  19. PDAC responds to radiotherapy by producing CCL2, which recruits Ly6C(+)CCR2(+) monocytes to support tumor proliferation and neovascularization after radiotherapy. PMID: 27354473
  20. Chemokine receptor 2 (CCR2(+)) monocytes invade the hippocampus between 1 and 3 d after SE. In contrast, only an occasional CD3(+) T lymphocyte was encountered 3 d after SE. The initial cellular sources of the chemokine CCL2, a ligand for CCR2, included perivascular macrophages and microglia. The induction of the proinflammatory cytokine IL-1beta was greater in FACS-isolated microglia than in brain-invading monocytes PMID: 27601660
  21. data suggest that MCP-1 may be a necessary component of the inflammatory response required for adipose tissue protection, remodeling and healthy expansion in the FVB/N strain in response to high-fat diet feedings PMID: 26620890
  22. MCP-1 knockout mice exposed for voluntary wheel running displayed changes in brain cytokine expression and glial activation. PMID: 28088610
  23. Carvedilol treatment also significantly reduced the levels of proinflammatory cytokines tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-6, transforming growth factor (TGF)-beta1 and monocyte chemoattractant protein (MCP)-1 in the lung tissue. PMID: 27619518
  24. High dilutions of antimony modulate cytokines production and macrophage - Leishmania (L.) amazonensis interaction in vitro.( PMID: 28092793
  25. Both IL-6 protein production and transcript levels were downregulated by RA in respiratory tract epithelial cells (LETs) , but upregulated in macrophages (MACs). RA also increased transcript levels of MCP-1, GMCSF, and IL-10 in MACs, but not in LETs. Conversely, when LETs, but not MACs, were exposed to RA PMID: 27940088
  26. FSAP deficiency causes an increase in CCL2 expression and CCL2-mediated infiltration of leukocytes into the injured vessel PMID: 27431088
  27. miR-33 suppresses CCL2 expression via targeting at the 3'UTR. The miR-33/CCL2 axis in chondrocytes regulates the monocyte chemotaxis. PMID: 27129293
  28. Sca-1(+) progenitor cells found to exhibit increased migration was associated with elevated levels of chemokines, CCL2 (chemokine (C-C motif) ligand 2) and CXCL1 (chemokine (C-X-C motif) ligand 1), and their corresponding receptors. PMID: 27300479
  29. that MCP-1 inhibition restores glomerular endothelial glycocalyx and barrier function and reduces tissue inflammation in the presence of ongoing diabetic injury PMID: 28837800
  30. In LLC-bearing mice, the high-fat diet increased and MCP-1 deficiency decreased plasma TRAP 5b; neither the high-fat diet nor MCP-1 deficiency resulted in significant changes in plasma concentration of osteocalcin. PMID: 28177896
  31. Grooved surfaces showed time-dependent increase in soluble mediators involved in cell fusion, CCL2 and MMP-9 PMID: 27102570
  32. In this study, the effect of continuous IL-4 delivery or bioactive implant coating that constitutively releases a protein inhibitor of CCL2 signaling (7ND) on particle induced osteolysis were studied in the murine continuous femoral intramedullary particle infusion model PMID: 27114284
  33. Blockade of MCP-1 activity significantly reduced inflammatory pleural effusion formation in a CAR model PMID: 27878909
  34. CCL2 recruits T cells into the brain in a CCR2-independent manner. PMID: 28836736
  35. Expression of MCP-1 induced by IL-17 requires NF-kappaB through the phosphorylation of p65 in cardiac myocytes. PMID: 28593659
  36. this study demonstrates that killed probiotics can reduce viral disease severity and identify that the MCP-1 pathway might act as a key mediator in the improved antiviral immune response PMID: 27072606
  37. tumor-promoting role for CCL2 acting through CCR2 on the tumor microenvironment PMID: 27820834
  38. type I interferons, besides their known antiviral properties, can initiate the recruitment and activation of leukocytes via induction of chemokine expression including CCL2. PMID: 26992431
  39. In cultured macrophages, LPS-induced expression of monocyte chemoattractant protein (MCP)-1 was suppressed by NaN3 through inhibition of STAT1 and IkappaBzeta activities. PMID: 28391993
  40. study concludes that CCL2 signaling onto melanin-concentrating hormone neurons represents a core mechanism that relays peripheral inflammation to sickness behavior PMID: 27733491
  41. The increased migratory phenotypes are dependent on secreted factors, including the cytokine interleukin-6 and the chemokine CCL2. We propose that Delta122p53 (and Delta133p53) acts in a similar manner to 'gain-of-function' mutant p53 proteins to promote migration, invasion and metastasis, which may contribute to poor survival in patients with Delta133p53-expressing tumours PMID: 26996665
  42. CCL5 paradoxically limits macrophage accumulation in the injured kidney during renin angiotensin system (RAS) activation by constraining the proinflammatory actions of CCL2. PMID: 27640148
  43. This study establishes ECL1i as the first allosteric inhibitor of CCR2 with functional selectivity. PMID: 26979087
  44. Constitutive expression of CCL2 by the mouse mammary epithelium induces a state of low level chronic inflammation that increases stromal density and elevates cancer risk. We propose that CCL2-driven inflammation contributes to the increased risk of breast cancer observed in women with high mammographic density. PMID: 28077158
  45. the individual and combined actions of CCL2/CCR2 and CX3CL1/CX3CR1 in hypoxia-induced pulmonary hypertension in mice, is reported. PMID: 28125278
  46. CCL2 acting at the suprachiasmatic nuclei level is important for the circadian effects of immune activation. PMID: 27178133
  47. FAP-STAT3-CCL2 signaling in Cancer-associated fibroblasts (CAF) was sufficient to program an inflammatory component of the tumor microenvironment, which may have particular significance in desmoplasia-associated cancers. PMID: 27216177
  48. Macrophages induce neural stem cells migrating into the spinal cord injury site possibly through promoting the secretion of MCP-1. PMID: 27609570
  49. this study shows that CCL2 supports the classical activation of macrophages, with miR-9 mediated down-regulation of Dusp6 and enhanced ERK-mediated signal transduction possibly mediating this enhanced pro-inflammatory gene expression PMID: 28242024
  50. macrophage-specific chemokine (C-C Motif) receptor 2 (Ccr2) knockout (M-Ccr2KO) and intestinal epithelial cell-specific tamoxifen-inducible Ccl2 knockout (Vil-Ccl2KO) mice, were studied. PMID: 27508875

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Subcellular Location Secreted.
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: mmu:20296

STRING: 10090.ENSMUSP00000000193

UniGene: Mm.290320

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