Pig Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

Pig Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

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VEGFA Antibodies

VEGFA Proteins

VEGFA Antibodies

VEGFA Proteins

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ELISA Kit
Pig Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

Specification
Target

Code

CSB-E12053p

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Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P49151

Lead Time

3-5 working days

Abbreviation

VEGFA

Protein Biological Process 1

Angiogenesis

Target Name

vascular endothelial growth factor A

Alias

RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor

Species

Sus scrofa (Pig)

Protein Biological Process 3

Angiogenesis

Sample Types

serum, plasma, tissue homogenates

Detection Range

4.69 pg/mL-300 pg/mL

Sensitivity

1.17 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	85-98
1:10	Average %	90
	Range %	85-97
1:20	Average %	96
	Range %	89-103
1:40	Average %	86
	Range %	81-92

Recovery

The recovery of pig VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	96	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.032	1.984	2.008	1.871
150	1.746	1.744	1.745	1.608
75	1.465	1.487	1.476	1.339
37.5	1.109	1.127	1.118	0.981
18.75	0.763	0.736	0.750	0.613
9.37	0.525	0.521	0.523	0.386
4.69	0.334	0.348	0.341	0.204
0	0.135	0.138	0.137

References

Placental growth factor 2-A potential therapeutic strategy for chronic myocardial ischemia Wu M. et al,Int J Cardiol,2016
Effect of collagen scaffold with adipose-derived stromal vascular fraction cells on diabetic wound healing: A study in a diabetic porcine model Yanan Jiang et al,Tissue Engineering and Regenerative Medicine,2013

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Function	Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).
Subcellular Location	Secreted
Protein Families	PDGF/VEGF growth factor family
Database Links	KEGG: ssc:397157 STRING: 9823.ENSSSCP00000001845 UniGene: Ssc.57541
Pathway	HIF-1 signaling pathway MAPK signaling pathway PI3K-Akt signaling pathway VEGF signaling pathway Focal adhesion Ras signaling pathway AGE-RAGE signaling pathway in diabetic complications Rap1 signaling pathway

VEGFA Antibody

VEGFA Protein

VEGFA Antibodies for Pig

Code	Product Name	Species Reactivity	Application
CSB-PA025833LC01PI	VEGFA Antibody, FITC conjugated	Pig	ELISA
CSB-PA025833LA01PI	VEGFA Antibody	Pig	ELISA
CSB-PA025833LD01PI	VEGFA Antibody, Biotin conjugated	Pig	ELISA
CSB-PA025833LB01PI	VEGFA Antibody, HRP conjugated	Pig	ELISA

VEGFA Proteins for Sus scrofa (Pig)

Code	Product Name	Source
CSB-YP025833PI CSB-BP025833PI CSB-MP025833PI	Recombinant Pig Vascular endothelial growth factor A(VEGFA)	Yeast Baculovirus Mammalian cell
CSB-EP025833PI	Recombinant Pig Vascular endothelial growth factor A(VEGFA)	E.coli

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Sensitivity: 0.39 ng/mL
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.