Pig Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

Instructions
Code CSB-E12053p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name vascular endothelial growth factor A
Alternative Names VEGFA ELISA kit; VEGFVascular endothelial growth factor A ELISA kit; VEGF-A ELISA kit; Vascular permeability factor ELISA kit; VPF ELISA kit
Abbreviation VEGFA
Uniprot No. P49151
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 4.69 pg/mL-300 pg/mL
Sensitivity 1.17 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %92
Range %85-98
1:10Average %90
Range %85-97
1:20Average %96
Range %89-103
1:40Average %86
Range %81-92
Recovery
The recovery of pig VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-99
EDTA plasma (n=4)9690-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
3002.032 1.984 2.008 1.871
1501.746 1.744 1.745 1.608
751.465 1.487 1.476 1.339
37.51.109 1.127 1.118 0.981
18.750.763 0.736 0.750 0.613
9.370.525 0.521 0.523 0.386
4.690.334 0.348 0.341 0.204
00.135 0.138 0.137  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).
Gene References into Functions
  1. It regulates endometrial remodeling in the porcine endometrial tissues during follicular and luteal phase. PMID: 28139071
  2. It was concluded that VEGF and factor VIII are important growth factors associated with fetal development in pigs and are identified in all uterine segments. PMID: 28237345
  3. results are consistent with a participation of (VEFG) in the regulation of the dynamics of oviductal fluid secretion and the oviduct contractibility PMID: 28257866
  4. Here we demonstrate that VEGF-165 mediates MSC differentiation into ECs via VEGFR-2-dependent induction of Sox18, which ultimately coordinates the transcriptional upregulation of specific markers of the EC phenotype. PMID: 25913202
  5. VEGF was significantly downregulated 7 days after cryotherapy of the sclera and stayed at that level until day 14. It returned to baseline by day 21. PMID: 25510786
  6. VEGF production, blood vessel network and follicle remodeling are impaired by the antiprogesterone RU486. PMID: 24756033
  7. findings suggest that the augmented neovascular response seen with VEGF administration was through the VEGF-induced upregulation of Notch signaling. PMID: 24366099
  8. interleukin-1beta-induced vascular endothelial growth factor in airway smooth muscle cells PMID: 24142467
  9. Upregulation of VEGF during hypoxia in chondrocyte is mediated partially through HIF-1alpha. PMID: 22970342
  10. data shows that members of the VEGF-VEGFR system are temporally and spatially well localized for playing key roles during umbilical cord formation and its intensive growth observed after day 75 of pregnancy PMID: 20825582
  11. cardiac-specific and hypoxia-induced coexpression of VEGF and Ang1 improves the perfusion and function of porcine MI heart through the induction of angiogenesis and cardiomyocyte proliferation PMID: 21245320
  12. The mRNA for VEGFA was expressed in early developing and in maturing glomeruli. PMID: 20713984
  13. Data show that subventricular zone neural stem cells express VEGF and all VEGF splice variants, VEGFR1, VEGFR2 and Neuropilin-1 and -2 mRNAs. PMID: 20552272
  14. The earlier increase in VEGF protein and mRNA expression in the Meishan versus the Yorkshire conceptus may explain the previously reported increased vascularity and increased placental efficiency of this breed compared the Yorkshire breed. PMID: 14998908
  15. Contrast media did not affect bone marrow cell viability/VEGF secretion. PMID: 15274157
  16. VEGF upregulation in the proliferative zone after ischemic damage may play a role in stimulating vascular invasion and granulation tissue formation in the necrotic hypertrophic zone of the epiphyseal cartilage PMID: 15537448
  17. Data demonstrate that lipopolysaccharides evoke a heat shock response, with an increase heat shock proteins 70 and Hsp32) and of VEGF, a specific endothelial cell growth factor. PMID: 16333987
  18. Vascular endothelial growth factor (VEGF) plays an important role in the thecal angiogenesis during follicular development. PMID: 16372043
  19. number of preovulatory follicles and the capillary density in the theca interna increased significantly in the ovaries injected with VEGF gene PMID: 16538032
  20. our study revealed the presence of VEGF in endothelial and smooth muscle cells of vascular subovarian plexus arteries PMID: 16584087
  21. Differential regulation of VEGF isoforms may suggest specific physiological roles for some of them, particularly in angioregression occurring during the apoptotic structural luteolysis. PMID: 16967516
  22. VEGF ligand-receptor system may play an important role in the development and maintenance of the corpus luteum in pigs. PMID: 17120307
  23. Sanguinarine inhibited both VEGF production and VEGF-induced Akt activation in swine granulosa cells. PMID: 17310078
  24. VEGF/Flk-1/Flt-1 system is activated during myocardial ischemia reperfusion injury. PMID: 17868880
  25. VEGF-A family members are probably involved in appropriate preparation of endometrium for implantation and in vascular events during implantation in pigs. PMID: 17874446
  26. Hemodialysis graft placement leads to early increases in wall shear stress, VEGF-A, pro-MMP-9, MMP-2, VEGFR-1, VEGFR-2, and TIMP-1, which may contribute to the development of venous stenosis. PMID: 18326810
  27. Interaction of soluble factors in plasma possibly generated during PICSO are not responsible for upregulation of HO-1 and VEGF mRNA. PMID: 19618599

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Subcellular Location Secreted
Protein Families PDGF/VEGF growth factor family
Database Links

KEGG: ssc:397157

STRING: 9823.ENSSSCP00000001845

UniGene: Ssc.57541

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