AKT2 (Ab-474) Antibody

Datasheet
Code CSB-PA215240
Product Type Polyclonal Antibody
Size US$297
Uniprot No. P31751
Image
  • Western blot analysis of extracts from 293 and PC12 cells using Akt2(Ab-474) Antibody.
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2(Ab-474) Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunogen Peptide sequence around aa. 472~476 (Q-F-S-Y-S) derived from Human Akt2.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of total Akt2 protein.
Tested Applications ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:200
Relevance General protein kinase capable of phosphorylating several known proteins.
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality Polyclonal
Alias PKB beta; Akt-2; Protein kinase B; RAC-PK-beta;
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names AKT2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development.; FUNCTION
Involvement in disease Diabetes mellitus, non-insulin-dependent (NIDDM); Hypoinsulinemic hypoglycemia with hemihypertrophy (HIHGHH)
Subcellular Location Cytoplasm, Nucleus, Cell membrane, Peripheral membrane protein, Early endosome
Protein Families Protein kinase superfamily, AGC Ser/Thr protein kinase family, RAC subfamily
Tissue Specificity Expressed in all cell types so far analyzed.
Database Links

HGNC: 392

OMIM: 125853

KEGG: hsa:208

STRING: 9606.ENSP00000375892

UniGene: Hs.631535

Pathway cAMP signaling pathway
Chemokine signaling pathway
ErbB signaling pathway
Estrogen signaling pathway
HIF-1 signaling pathway
Jak-STAT signaling pathway
MAPK signaling pathway
mTOR signaling pathway
PI3K-Akt signaling pathway
VEGF signaling pathway
Focal adhesion
Lipids and Inflammation in Atherogenesis
TNF signaling pathway
Apoptosis
Autophagy
Cellular senescence
Ras signaling pathway
Signaling pathways regulating pluripotency of stem cells
B cell receptor signaling pathway
Fc epsilon RI signaling pathway
Fc gamma R-mediated phagocytosis
Osteoclast differentiation
T cell receptor signaling pathway
Toll-like receptor signaling pathway
Adipocytokine signaling pathway
AGE-RAGE signaling pathway in diabetic complications
AMPK signaling
Apelin signaling pathway
cGMP-PKG signaling pathway
FoxO signaling pathway
Glucagon signaling pathway
Insulin signaling pathway
Oxidative Phosphorylation
Phospholipase D signaling pathway
Rap1 signaling pathway
Neurotrophin signaling pathway

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