Recombinant Burkholderia cenocepacia Multifunctional CCA protein(cca)

Product Type Recombinant Protein
Uniprot No. A0KBA2
Species Burkholderia cenocepacia (strain HI2424)
Gene Names cca
Protein Names Recommended name: Multifunctional CCA protein Including the following 4 domains: CCA-adding enzyme EC= 2.7.7.72 Alternative name(s): CCA tRNA nucleotidyltransferase tRNA CCA-pyrophosphorylase tRNA adenylyl-/cytidylyl-transfe
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. Also shows phosphatase, 2'-nucleotidase and 2',3'-cyclic phosphodiesterase activities. These phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases.
Protein Families TRNA nucleotidyltransferase/poly(A) polymerase family, Bacterial CCA-adding enzyme type 1 subfamily
Database Links

KEGG: bch:Bcen2424_3031

Product Details for Different Expression Systems

Product Name Recombinant Burkholderia cenocepacia Multifunctional CCA protein(cca),Yeast
Code CSB-YP368411BPO
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MNIYAVGGAI RDELLGVPVQ DRDYVVVGAT PEQMTAQGFR AVGKDFPVFL HPQTQEEYAL ARTERKTAAG YHGFQFHYAP DVTLDEDLAR RDLTINAMAR EVSPEGALVG PVIDPFDGQA DLRARVFRHV SDAFVEDPVR ILRIARFAAR FADFTVADET LALMRRMVDA GEVDALVPER VWQEIARGLM EAKPSRMFAV LRDCGALARI LPEVDALWGV PQRADYHPEV DTGVHVMMVV DYAAKQGYSL AVRFAALTHD LGKGTTPADV LPRHVGHESR SVELLKPLCE RLRVPNECRD LALVVAREHG NLHRVMEMGA AALVRLFERS DALRKPARFA ELLQACESDA RGRLGLDAQP YPQAERLRVA LAAARSVDAG AIARGIGNDT EKIKEAVHRA RIQAVAQALA IGE
Expression Region 1-413
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
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Product Name Recombinant Burkholderia cenocepacia Multifunctional CCA protein(cca),E.coli
Code CSB-EP368411BPO
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MNIYAVGGAI RDELLGVPVQ DRDYVVVGAT PEQMTAQGFR AVGKDFPVFL HPQTQEEYAL ARTERKTAAG YHGFQFHYAP DVTLDEDLAR RDLTINAMAR EVSPEGALVG PVIDPFDGQA DLRARVFRHV SDAFVEDPVR ILRIARFAAR FADFTVADET LALMRRMVDA GEVDALVPER VWQEIARGLM EAKPSRMFAV LRDCGALARI LPEVDALWGV PQRADYHPEV DTGVHVMMVV DYAAKQGYSL AVRFAALTHD LGKGTTPADV LPRHVGHESR SVELLKPLCE RLRVPNECRD LALVVAREHG NLHRVMEMGA AALVRLFERS DALRKPARFA ELLQACESDA RGRLGLDAQP YPQAERLRVA LAAARSVDAG AIARGIGNDT EKIKEAVHRA RIQAVAQALA IGE
Expression Region 1-413
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Burkholderia cenocepacia Multifunctional CCA protein(cca),Baculovirus
Code CSB-BP368411BPO
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MNIYAVGGAI RDELLGVPVQ DRDYVVVGAT PEQMTAQGFR AVGKDFPVFL HPQTQEEYAL ARTERKTAAG YHGFQFHYAP DVTLDEDLAR RDLTINAMAR EVSPEGALVG PVIDPFDGQA DLRARVFRHV SDAFVEDPVR ILRIARFAAR FADFTVADET LALMRRMVDA GEVDALVPER VWQEIARGLM EAKPSRMFAV LRDCGALARI LPEVDALWGV PQRADYHPEV DTGVHVMMVV DYAAKQGYSL AVRFAALTHD LGKGTTPADV LPRHVGHESR SVELLKPLCE RLRVPNECRD LALVVAREHG NLHRVMEMGA AALVRLFERS DALRKPARFA ELLQACESDA RGRLGLDAQP YPQAERLRVA LAAARSVDAG AIARGIGNDT EKIKEAVHRA RIQAVAQALA IGE
Expression Region 1-413
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Burkholderia cenocepacia Multifunctional CCA protein(cca),Mammalian cell
Code CSB-MP368411BPO
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MNIYAVGGAI RDELLGVPVQ DRDYVVVGAT PEQMTAQGFR AVGKDFPVFL HPQTQEEYAL ARTERKTAAG YHGFQFHYAP DVTLDEDLAR RDLTINAMAR EVSPEGALVG PVIDPFDGQA DLRARVFRHV SDAFVEDPVR ILRIARFAAR FADFTVADET LALMRRMVDA GEVDALVPER VWQEIARGLM EAKPSRMFAV LRDCGALARI LPEVDALWGV PQRADYHPEV DTGVHVMMVV DYAAKQGYSL AVRFAALTHD LGKGTTPADV LPRHVGHESR SVELLKPLCE RLRVPNECRD LALVVAREHG NLHRVMEMGA AALVRLFERS DALRKPARFA ELLQACESDA RGRLGLDAQP YPQAERLRVA LAAARSVDAG AIARGIGNDT EKIKEAVHRA RIQAVAQALA IGE
Expression Region 1-413
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat

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