Mouse Insulin,INS ELISA Kit

Code CSB-E05071m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01325
Lead Time 3-5 working days
Abbreviation INS
Target Name Insulin,INS
Alias N/A
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 15.6 nIU/mL-1000 nIU/mL
Sensitivity 3.9 nIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 90  
Range % 84-95  
1:200 Average % 93  
Range % 90-96  
1:400 Average % 95  
Range % 90-100  
1:800 Average % 94  
Range % 87-99  
The recovery of mouse INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 92-102  
EDTA plasma (n=4) 89 82-96  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nIU/ml. OD1 OD2 Average Corrected  
1000 2.546 2.598 2.572 2.396  
500 2.231 2.267 2.249 2.073  
250 1.745 1.769 1.757 1.581  
125 1.198 1.163 1.181 1.005  
62.5 0.648 0.628 0.638 0.462  
31.2 0.426 0.407 0.417 0.241  
15.6 0.302 0.326 0.314 0.138  
0 0.173 0.179 0.176    
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Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: mmu:16333

STRING: 10090.ENSMUSP00000049095

UniGene: Mm.46269

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