Mouse Insulin,INS ELISA Kit

Code CSB-E05071m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01325
Lead Time 3-5 working days
Abbreviation INS
Target Name Insulin,INS
Alias N/A
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 15.6 nIU/mL-1000 nIU/mL
Sensitivity 3.9 nIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 90  
Range % 84-95  
1:200 Average % 93  
Range % 90-96  
1:400 Average % 95  
Range % 90-100  
1:800 Average % 94  
Range % 87-99  
Recovery
The recovery of mouse INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 92-102  
EDTA plasma (n=4) 89 82-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nIU/ml. OD1 OD2 Average Corrected  
1000 2.546 2.598 2.572 2.396  
500 2.231 2.267 2.249 2.073  
250 1.745 1.769 1.757 1.581  
125 1.198 1.163 1.181 1.005  
62.5 0.648 0.628 0.638 0.462  
31.2 0.426 0.407 0.417 0.241  
15.6 0.302 0.326 0.314 0.138  
0 0.173 0.179 0.176    
References
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Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: mmu:16333

STRING: 10090.ENSMUSP00000049095

UniGene: Mm.46269

Q&A and Customer Reviews

Customer Reviews
  • Sample type: Plasma
    Sample species: Mouse
    Review: Mice were treated for 35 weeks (=5 cycles) or 56 weeks (=8 cycles), blood was collected from the heart into EDTA tubes, centrifuged and plasma analysed for insulin. Values are given as mean ± SEM. Values in parenthesis give the number of analysed animals.
    PMID: 30664922
  • Sample type: Serum
    Sample species: Mouse
    Review: Serum insulin levels at the end of 5-week administration. Data are presented as mean ± SEM. **p < 0.01 and *p < 0.05 versus model group, respectively. Normal, normal mice group; Model, T2D mice group; Met, T2D mice group administrated with metformin at 200 mg/kg/day; GXG-H, T2D mice group administrated with 200 mg/kg/day; GXG-L, T2D mice group administrated with 50 mg/kg/day.
    PMID: 30824102
  • Sample type: Serum
    Sample species: Mouse
    Review: Effects of BDE-209 on serum insulin concentration in mice. Insulin level in serum treated with various dosages of BDE-209. *p < 0.05, compared with control group.
    PMID: 30743236
  • Sample type: Serum
    Sample species: Mouse
    Review: Polytherapy with reserpine and GLP-1 increases serum insulin levels in STZ-induced diabetes. Note that both, monotherapy with reserpine and polytherapy with GLP-1/reserpine elevated serum levels of insulin compared to diabetic and healthy control mice.
  • Sample type: Serum
    Sample species: Mouse
    Review: Compared with the DM mice with OVX, ER agonists including E2, PPT and DPN treatment did not affect the levels of serum insulin ( F(6,27) = 17.240, p < 0.001 for serum insulin)
    PMID: 30201537
  • Sample type: Serum
    Sample species: Mouse
    Review: The insulin level is compared on the 18th day of pregnancy. The results showed that mice in the normal group had significantly higher FINS in comparison to those in the normal group.
    PMID: 30341900
  • Sample type: Serum
    Sample species: Mouse
    Review: compared with db/+ mice, fasting serum insulin (F[3,20] = 57.196, P<0.01) increased significantly in db/db mice.
    PMID: 29248482
  • Sample type: Serum
    Sample species: Mouse
    Review: Insulin levels tended to be higher in the 35 weeks treatment group (5 cycles) and became significant in the 56 weeks treatment group (8 cycles) compared to the controls.
    PMID: 30823598

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