Mouse Insulin,INS ELISA Kit

Code CSB-E05071m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Insulin,INS
Alternative Names Ins1 ELISA Kit; Ins-1 ELISA Kit; Insulin-1 [Cleaved into: Insulin-1 B chain; Insulin-1 A chain] ELISA Kit
Abbreviation INS
Uniprot No. P01325
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 15.6 nIU/mL-1000 nIU/mL
Sensitivity 3.9 nIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 90  
Range % 84-95  
1:200 Average % 93  
Range % 90-96  
1:400 Average % 95  
Range % 90-100  
1:800 Average % 94  
Range % 87-99  
The recovery of mouse INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 92-102  
EDTA plasma (n=4) 89 82-96  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nIU/ml. OD1 OD2 Average Corrected  
1000 2.546 2.598 2.572 2.396  
500 2.231 2.267 2.249 2.073  
250 1.745 1.769 1.757 1.581  
125 1.198 1.163 1.181 1.005  
62.5 0.648 0.628 0.638 0.462  
31.2 0.426 0.407 0.417 0.241  
15.6 0.302 0.326 0.314 0.138  
0 0.173 0.179 0.176    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


Q&A and Customer Reviews

 Customer Reviews
  • Sample type: Plasma
    Sample species: Mouse
    Review: Mice were treated for 35 weeks (=5 cycles) or 56 weeks (=8 cycles), blood was collected from the heart into EDTA tubes, centrifuged and plasma analysed for insulin. Values are given as mean ± SEM. Values in parenthesis give the number of analysed animals.
    PMID: 30664922
  • Sample type: Serum
    Sample species: Mouse
    Review: Serum insulin levels at the end of 5-week administration. Data are presented as mean ± SEM. **p < 0.01 and *p < 0.05 versus model group, respectively. Normal, normal mice group; Model, T2D mice group; Met, T2D mice group administrated with metformin at 200 mg/kg/day; GXG-H, T2D mice group administrated with 200 mg/kg/day; GXG-L, T2D mice group administrated with 50 mg/kg/day.
    PMID: 30824102
  • Sample type: Serum
    Sample species: Mouse
    Review: Effects of BDE-209 on serum insulin concentration in mice. Insulin level in serum treated with various dosages of BDE-209. *p < 0.05, compared with control group.
    PMID: 30743236
  • Sample type: Serum
    Sample species: Mouse
    Review: Polytherapy with reserpine and GLP-1 increases serum insulin levels in STZ-induced diabetes. Note that both, monotherapy with reserpine and polytherapy with GLP-1/reserpine elevated serum levels of insulin compared to diabetic and healthy control mice.
  • Sample type: Serum
    Sample species: Mouse
    Review: Compared with the DM mice with OVX, ER agonists including E2, PPT and DPN treatment did not affect the levels of serum insulin ( F(6,27) = 17.240, p < 0.001 for serum insulin)
    PMID: 30201537
  • Sample type: Serum
    Sample species: Mouse
    Review: The insulin level is compared on the 18th day of pregnancy. The results showed that mice in the normal group had significantly higher FINS in comparison to those in the normal group.
    PMID: 30341900
  • Sample type: Serum
    Sample species: Mouse
    Review: compared with db/+ mice, fasting serum insulin (F[3,20] = 57.196, P<0.01) increased significantly in db/db mice.
    PMID: 29248482
  • Sample type: Serum
    Sample species: Mouse
    Review: Insulin levels tended to be higher in the 35 weeks treatment group (5 cycles) and became significant in the 56 weeks treatment group (8 cycles) compared to the controls.
    PMID: 30823598

Target Data

Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Gene References into Functions
  1. Impairment in the insulin-Snail1 axis may contribute to non-alcoholic fatty liver disease in obesity. PMID: 30013137
  2. Feeding of high fat diet leads to impairment of brain insulin signaling linked with neuroinflammation. Insulin resistance due to high fat diet associated with biochemical changes in markers related with Alzheimer disease pathology. PMID: 27771511
  3. High INS1 expression is associated with weight gain and obesity. PMID: 29122848
  4. psychological stress impairs insulin signaling and results in hippocampal deficits. PMID: 29970188
  5. Our data shed light on the putative role of Kv1.3 in weight gain and insulin-dependent responses contributing to knowledge about adipocyte physiology. PMID: 29947924
  6. Data suggest that hybrid insulin peptides (HIPs), formed in insulin-secreting-cells by fusion of insulin C-peptide fragments to peptide fragments of chromogranin A or islet amyloid polypeptide, and reactivity of CD4+-T-lymphocytes to HIPs may act as biomarkers of autoimmunity in type 1 diabetes. PMID: 29976617
  7. Our findings, focusing on energy balance, provide a mechanistic understanding of the promising effect of early insulin initiation on lipotoxicity. Insulin, by recovering UCP3 activity, alleviated energy surfeit and potentiated AMPK-mediated lipid homeostasis in skeletal muscle cells following exposure to PA and in gastrocnemius of mice fed HFD. PMID: 29039450
  8. In the present study, the mRNA expression of the two mouse insulin genes Ins1 and Ins2 was investigated in MIN6 cells treated with different concentrations of melatonin, and insulin secretion was detected under the same conditions. Following the overexpression or silencing of MTNR1B, the activities of components of the MAPK signaling pathway PMID: 29207116
  9. diabetic gastroparesis was an aggressive process due to the successive damages of myenteric cholinergic neurones and ICC by impairing the insulin/InsR and IGF-1/IGF-1R signaling. Insulin therapy in the early stage may delay diabetic gastroparesis PMID: 28931726
  10. nNOS mediates insulin- and oxidative stress-induced glucose uptake in skeletal muscle myotubes. PMID: 28666850
  11. Data (including data from studies using knockout mice) suggest that Ins1 and Ins2 are required for pancreatic beta-cell maturation; thus, Ins1 and Ins2 are needed for normal beta-cell development and for maintenance of normal beta-cell function. PMID: 29029025
  12. Despite higher endogenous insulin concentrations following feeding, arcuate nucleus phosphorylation of Akt (pAkt) levels were significantly lower in the pregnant group compared with the nonpregnant group. PMID: 29029017
  13. Our current results reinforce the notion that the AT2R has a physiological role in the conservation of insulin action PMID: 27979738
  14. E4-ORF1 activation of PI3K in adipocytes recapitulates insulin regulation of FoxO1 but not regulation of Glut4. This uncoupling of PI3K effects occurs despite E4-ORF1 activating PI3K and downstream signaling to levels achieved by insulin PMID: 28009298
  15. These data support a role for islet NGF in fine-tuning insulin secretion. PMID: 27424144
  16. PDX1 and ISL1 regulation of insulin gene expression in pancreatic beta cells, was investigated. PMID: 26994512
  17. insulin and aPC converge on a common spliced-X-box binding protein-1 (sXBP1) signaling pathway to maintain endoplasmic reticulum (ER) homeostasis. PMID: 28687614
  18. Insulin stimulation of Akt1 and Akt2 signaling in Cystic fibrosis airway cells was diminished compared with that observed in airway cells expressing wild-type CFTR. PMID: 28213469
  19. These data implicate the insulin-FoxM1/PLK1/CENP-A pathway-regulated mitotic cell-cycle progression as an essential component in the beta cell adaptation to delay and/or prevent progression to diabetes. PMID: 28286049
  20. Rac1 activation is caused by membrane translocation of a guanine nucleotide exchange factor FLJ00068 in Akt2-mediated insulin signaling in mouse skeletal muscle. PMID: 27163697
  21. Netrin-1 enhanced insulin secretion by promoting beta-cell Ca(2+) influx and cAMP production. PMID: 27520508
  22. This study identifies AR as a novel receptor that enhances beta cell function. PMID: 27133133
  23. The effects were abolished by using pharmacological inhibition of PI3K/Akt with LY294002 and paralleled by transfecting DCs with klotho siRNA. In conclusion, the regulation of klotho sensitive DC function by IGF-1 or insulin is mediated through PI3K/Akt signaling pathway in BMDCs. PMID: 27808000
  24. Overexpression of either ca-Nfatc2 or ca-Nfatc1 in mouse islets enhanced insulin secretion, whereas only ca-Nfatc2 was able to promote b-cell proliferation, suggesting distinct molecular pathways mediating insulin secretion vs. b-cell proliferation are regulated by NFAT PMID: 27935966
  25. BMP-7 therefore is an attractive candidate for tackling a multifaceted disease such as diabetes, since it not only reduces body fat, but also strengthens insulin signaling, causing improved glucose uptake and ameliorating peripheral insulin resistance. PMID: 28186649
  26. these findings demonstrate, for the first time, that miR-155 is a positive regulator of insulin sensitivity with potential applications for diabetes treatment. PMID: 27711113
  27. Maternal chromium restriction leads to glucose metabolism imbalance in mice offspring through insulin signaling and Wnt signaling pathways. PMID: 27782077
  28. Hyperglycemia and hyperlipidemia blunts the Insulin-Inpp5f negative feedback loop in the diabetic heart. PMID: 26908121
  29. Data suggest that resveratrol acts on differentiating preadipocytes by inhibiting insulin signaling, mitochondrial biogenesis, and lipogenesis. PMID: 26968895
  30. elevating nuclear O-GlcNAc increases intracellular insulin levels and preserves glucose-stimulated insulin secretion during chronic hyperglycemia PMID: 26598517
  31. Data (including data from studies in knockout/transgenic mice) suggest INS is required for lipogenic effects of activation of LXRalpha in liver; INS is not required to down-regulate gene expression in ER stress or inflammation (as seen in diabetes). PMID: 26511317
  32. the insulin-InsR signaling drives multipotent progenitors differentiation into lymphoid lineages in early lymphopoiesis. PMID: 26573296
  33. The target miRNAs are closely associated with dysregulation of insulin/PI3K-AKT signaling, suggesting that the Cmah-null mice could be a useful model for studying diabetes. PMID: 25243123
  34. the inhibitory effect of CRFR2 signaling on insulin action is mediated by cAMP in a mammalian target of rapamycin-dependent manner. PMID: 25875045
  35. This study discloses age-dependent changes in insulin CSF/serum ratios in humans. In the elderly, cerebral insulin resistance might be partially attributed to an impaired transport of insulin into the central nervous system PMID: 25965336
  36. adiponectin. Taken together, our results show that adiponectin is stored in a unique vesicular compartment, and released through a regulated exocytosis pathway that is dependent on insulin signalling. PMID: 26330614
  37. LKB1 is essential for mitochondrial maintenance and negatively regulates a distal step of insulin secretion. PMID: 26139601
  38. ubiquitin-like protein 4A (Ubl4A) plays a crucial role in insulin-induced Akt plasma membrane translocation. PMID: 26195787
  39. synaptotagmin-7 is directly activated by GLP-1 signaling and may serve as a drug target for boosting insulin secretion. PMID: 26216970
  40. The paracrine actions of Ucn3 activate a negative feedback loop that promotes somatostatin release to ensure the timely reduction of insulin secretion upon normalization of plasma glucose. PMID: 26076035
  41. Tcf7l2 is regulating proinsulin expression directly via Isl1, Ins1 and indirectly via MafA, NeuroD1 and Pdx1. PMID: 25015099
  42. elevated adiponectin levels improve systemic lipid metabolism in the near absence of insulin. PMID: 25339419
  43. the RhoA/ROCK signaling pathway is involved in insulin release through the up-regulation of Cx36 expression in 3D-cultured MIN6 cells. PMID: 25129107
  44. Analyzed was insulin translation in islets and in INS-1 cells. Insulin translational levels were significantly increased in islets of mice fed a high-fat diet to meet systemic demand, without altering its transcriptional levels. PMID: 25686499
  45. Data indicate that Src homology-2 domain containing protein B (SHB) deficiency causes a chronic increase in beta-cell focal adhesion kinase (FAK) activity that perturbs the normal insulin secretory characteristics of beta-cells. PMID: 25274988
  46. The activation of Cav-1 during the adipocyte differentiation process could facilitate the maintenance of insulin sensitivity by mature adipocytes isolated from additional external stimuli. PMID: 24751908
  47. GX sPLA2 negatively regulated pancreatic insulin secretion by augmenting COX-2-dependent PGE2 production. PMID: 25122761
  48. Diet-induced obesity mice exhibited significant increases in body weight, plasma glucose, insulin, and IGF1. PMID: 24914941
  49. The over-expression of miR-200a in the hypothalamus of obese mice is linked to leptin and insulin signaling impairment. PMID: 24394757
  50. Mouse Ins2 and Ins1 promoters were transiently activated in mouse fetal hepatocytes of embryonic days 13.5 and 16.5, respectively. PMID: 24258027
  51. Data suggest that CaMKII is a beta-cell Ca2+ sensor that amplifies secretagogue-induced Ca2+ entry and insulin secretion to maintain glucose homeostasis. PMID: 24627477
  52. Data suggest that alkali metals potassium, rubidium, or cesium concentration-dependently transform the pattern of calcium influx into pancreatic beta-cells and may thus generate stimuli of supraphysiological strength for insulin secretion. PMID: 24452455
  53. The lack of normal insulin signaling in Agpat2(-/-) livers allows unrestricted phosphatidic acid-induced gluconeogenesis. PMID: 24425876
  54. Girdin was identified as a new and major regulator of the insulin signal in myoblasts and skeletal muscle. PMID: 23886629
  55. Data suggest that CD34 may be a specific marker for functionality, with some specificity for insulin. PMID: 23973805
  56. Using all-atom structural models this work endeavored to understand the conformational reorganization of insulin on binding to its receptor in the presence of a recently resolved critical tandem hormone-binding element, the CT-peptide. PMID: 23348915
  57. These results suggest that autophagy is differentially regulated by insulin and amino acids in a tissue-dependent manner. PMID: 23744068
  58. B-cell translocation gene 2 (BTG2) is a crucial regulator in GLP-1-induced insulin gene expression and insulin secretion via upregulation of pancreatic duodenal homeobox-1 (PDX-1) in pancreatic beta-cells. PMID: 23703573
  59. Increased adiposity and insulin level is associated with the suppression of pulsatile GH secretion during weight gain. PMID: 23708999
  60. Selective serotonin reuptake inhibitors (SSRIs) inhibit insulin secretion and action in pancreatic beta cells PMID: 23275337
  61. Data suggest that C-reactive protein (CRP) attenuates insulin-induced skeletal muscle blood flow; CRP alters insulin resistance via IgG receptor IIB-mediated inhibition of glucose delivery to skeletal muscle. PMID: 23069625
  62. a crosstalk of leptin and insulin in the brain which leads to a decline in locomotor activity PMID: 22759970
  63. The aim of present study is to evaluate the effects of long-term high-fat diet on the regulation of the insulin-sensitizing fibroblast growth factor-21 (FGF-21) and visfatin in ApoE(-/-) mice. PMID: 22542568
  64. Klotho enhances glucose-induced insulin secretion by up-regulating plasma membrane levels of TRPV2 in MIN6 beta-cells PMID: 22597535
  65. Insulin promotes neuronal survival via the alternatively spliced protein kinase CdeltaII isoform. PMID: 22275369
  66. Genetic ablation of the sweet TR protein T1R2 obliterates fructose-induced insulin release and its potentiating effects on glucose-stimulated insulin secretion in vitro and in vivo. PMID: 22315413
  67. RORgamma controls adipogenesis and adipocyte size and modulates insulin sensitivity in obesity. PMID: 21853531
  68. Data show that when injected in utero into living mouse embryos, TAT-MafA significantly up-regulates target genes and induces enhanced insulin production as well as cytoarchitectural changes consistent with faster islet maturation. PMID: 21857924
  69. Estrogen-related receptor gamma (ERRgamma) is a novel transcriptional regulator of phosphatidic acid phosphatase, LIPIN1, and inhibits hepatic insulin signaling PMID: 21911493
  70. the control of precursor maturation and disposal acts as an early regulative mechanism in normal insulin production, and its disorder is crucially linked to beta-cell failure and diabetes pathogenesis PMID: 21559376
  71. Knockdown of the IRE1alpha gene prevents the insulin-mediated activation of the lipogenic program in hepatoma cells. PMID: 21447637
  72. Human IGFBP-3 overexpression impairs glucose regulation in mice via an inhibition of insulin secretion. PMID: 21447640
  73. PDE3B and the anti-lipolytic action of insulin may have a role in increased S-nitrosylation of adipose tissue in obesity PMID: 21724851
  74. These results suggest that increased KCNQ1 protein expression limits insulin secretion from pancreatic beta-cells by regulating the potassium channel current. PMID: 21426901
  75. Insulin-stimulated GLUT4 protein translocation in adipocytes requires the Rab10 guanine nucleotide exchange factor Dennd4C PMID: 21454697
  76. CDKAL1 controls first-phase insulin exocytosis in beta cells by facilitating ATP generation, K(ATP) channel responsiveness and the subsequent activity of Ca(2+) channels through pathways other than CDK5-mediated regulation PMID: 21151568
  77. nuclear import of FoxO1 contributes to the suppression of Pdx1 and Ins2 gene expression at low glucose, the latter via a previously unsuspected and direct physical interaction with the Ins2 promoter. PMID: 21335550
  78. SMS1 is important for controlling ROS generation, and that SMS1 is required for normal mitochondrial function and insulin secretion in pancreatic beta-cells PMID: 21115496
  79. Examination of "normal" insulin-responsive podocytes in vivo and in vitro demonstrates that insulin signals through the MAPK and PI3K pathways via the insulin receptor and directly remodels the actin cytoskeleton of this cell. PMID: 20889126
  80. M(3)-muscarinic receptor-mediated augmentation of sustained insulin release is largely independent of G protein-coupling but involves phosphorylation-/arrestin-dependent coupling of the receptor to protein kinase D1 PMID: 21078968
  81. These results demonstrate an initial spatiotemporal mapping of a dynamic protein-protein interaction on individual secretory granules that is linked to a specific granule behavior in living cells. PMID: 20713017
  82. Adiponectin-induced ERK and Akt phosphorylation protects against pancreatic beta cell apoptosis and increases insulin gene expression and secretion PMID: 20709750
  83. SREBP-1c mediates the effect of insulin on the expression of Cidea in mouse hepatocytes. PMID: 20575761
  84. Quantify the temporal pattern of initiation and expression of insulin genes during development of pancreatic islet cells. PMID: 19812524
  85. Data show that PCSK9-nullmice carried more LDLR and less insulin in their pancreas. PMID: 20026049
  86. Studies indicate that insulin/IGF-I and other genes converge and play partially overlapping roles in regulating aging and stress resistance in higher eukaryotes. PMID: 20098959
  87. Proinsulin C-peptide regulates ribosomal RNA expression PMID: 19917601
  88. insulin promoter CpG demethylation may play a crucial role in beta cell maturation and tissue-specific insulin gene expression PMID: 19742322
  89. Treatment with celecoxib increased insulin release from INS-1E cultured cells in a dose-dependent manner in glucose concentrations ranging from 5 to 17 mM. PMID: 12397372
  90. STAT 5 proteins are not activated by physiological levels of insulin in adipose tissue. PMID: 12604355
  91. the proximal insulin promoter is hyperacetylated at histone H3 and hypermethylated at H3-K4 in beta cells PMID: 12711597
  92. insulin increases the level of M2-PK mRNA in adipocytes by acting at transcriptional and post-transcriptional levels through signaling pathways involving both PI3K and MAPK kinase PMID: 12843653
  93. Insulin inhibition of the expression of IGFBP-1, PEPCK, and G6Pase genes occurs through different regulatory pathways PMID: 12914928
  94. Insulin up-regulates and leptin down-regulates adipocyte fatty acid uptake, leading to alterations in fatty acid partitioning that affect adiposity. PMID: 12949354
  95. an insulin splice-variant overexpressed in diabetes, obesity, and insulin resistance results in increased insulin translation PMID: 15550470
  96. Ins1 locus is unlikely to be imprinted in common laboratory mice PMID: 15567729
  97. the degradation of C-peptide in kidney and placenta follows similar patterns, dominated by endopeptidase cleavages N-terminal of Leu PMID: 15583859
  98. increased hepatocyte CYP2E1 expression and the presence of steatohepatitis result in the down-regulation of insulin signaling PMID: 15632182
  99. Pdx-1 directly regulates insulin transcription through formation of a complex with transcriptional coactivators on the proximal insulin promoter PMID: 15743769
  100. proinsulin/insulin molecules have a sequence that is a primary target of the autoimmunity that causes diabetes of the non-obese diabetic (NOD) mouse PMID: 15889095

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Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: mmu:16333

STRING: 10090.ENSMUSP00000049095

UniGene: Mm.46269

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