Rat luteinizing hormone (LH) Elisa kit

Instructions
Code CSB-E12654r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name luteinizing hormone
Alternative Names LhbLutropin subunit beta ELISA Kit; Lutropin beta chain ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta ELISA Kit
Abbreviation LH
Uniprot No. P01230
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 0.3 mLU/mL-60 mLU/mL
Sensitivity 0.15 mLU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 88-100  
1:2 Average % 95  
Range % 91-101  
1:4 Average % 94  
Range % 85-105  
1:8 Average % 96  
Range % 90-102  
Recovery
The recovery of rat LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 93-107  
EDTA plasma (n=4) 96 91-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average    
60 0.150 0.153 0.152    
15 0.352 0.338 0.345    
4.8 0.513 0.549 0.531    
1.2 0.985 1.002 0.994    
0.3 1.476 1.441 1.459    
0 2.679 2.654 2.667    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Gene References into Functions
  1. Orchestrated Egr1 and Pitx1 activities depending on specific central and peripheral regulatory inputs could be responsible for physiologically variable Lhb gene promoter activation in vivo. PMID: 25258388
  2. GnRH stimulates AMPK, and AMPK inhibition suppresses GnRH-stimulated LHbeta transcription. PMID: 23518923
  3. data suggest that the molecular decision for LHbeta subunit to enter the regulated secretory pathway is a pre-Golgi event controlled by the C-terminal heptapeptide. PMID: 23734233
  4. increased LH concentration induces production of incomplete LHR transcripts in hippocampal cells and decreases immature LHR at the protein level PMID: 23678542
  5. Data suggest that LH beta subunit expression (and FSH beta subunit expression) is regulated by DUSP1 expression (specifically in response to high-frequency GnRH pulses); this effect may occur in association with MAPK3/1 phosphorylation. PMID: 21228211
  6. Complex interactions between Ahr and Esr alter Prl and luteinizing hormone (LH) synthesis by direct actions in lactotropes and gonadotropes. PMID: 21187122
  7. LHbeta promoter activity is further regulated by the orphan nuclear receptors, chicken ovalbumin upstream promoter-transcription factors (COUP-TFI and COUP-TFII). PMID: 20797425
  8. data suggest that LHbeta G1502A polymorphism may be involved in the predisposition to infertility and minimal/mild endometriosis-associated infertility, although endometriosis might be only a coincidental finding along with infertility. PMID: 20430510
  9. PACAP may be released from the anterior pituitary cells in a concentration which would be able to influence LH release not only in vitro but under in vivo conditions as well. PMID: 12409218
  10. These results suggest that GnRH directly regulates LH secretion as well as LHbeta-subunit synthesis, and that LH2 cells are a useful model for the study of LH secretion induced by several secretagogues. PMID: 12940464
  11. androgens acting directly on the pituitary increase FSH-beta and decrease LH-beta transcription PMID: 14512439
  12. identify a second functional Pitx1 region spanning positions -73 to -52 in the LHbeta gene promoter PMID: 16087728
  13. JNK regulates both LHbeta and FSHbeta transcription in a differential manner. PMID: 17932215
  14. LHbeta, FSHbeta and the common alpha-subunit mRNA's were detected in intact follicles, theca cells, corpora lutea and in meiotically competent and incompetent oocytes. PMID: 18248883
  15. the carboxyl-terminal sequence in the lutropin beta subunit contributes to the sorting of lutropin to the regulated pathway PMID: 18292086
  16. In the 14-day-old rat pituitary, the majority of GHRH target cells are cells expressing LH beta mRNA alone or in combination with GH and/or POMC mRNA. PMID: 18363807
  17. Results demonstrate that degradation via the proteasome is vital to GnRH-stimulated LHbeta expression, and this occurs in part by allowing proper transcription factor associations with the LHbeta promoter. PMID: 19095772
  18. Lhb gene transcription in rat pituitary requires GnRH-induced early growth response 1 gene expression, which is mediated by MAPK8/9 and MAPK1/3. PMID: 19710510

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Subcellular Location Secreted
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: rno:25329

STRING: 10116.ENSRNOP00000060360

UniGene: Rn.10108

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