Rat luteinizing hormone (LH) Elisa kit

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Rat luteinizing hormone (LH) Elisa kit

Specification
Target

Code

CSB-E12654r

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P01230

Lead Time

3-5 working days

Abbreviation

Target Name

luteinizing hormone

Alias

N/A

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma

Detection Range

0.3 mLU/mL-60 mLU/mL

Sensitivity

0.15 mLU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-100
1:2	Average %	95
	Range %	91-101
1:4	Average %	94
	Range %	85-105
1:8	Average %	96
	Range %	90-102

Recovery

The recovery of rat LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-107
EDTA plasma (n=4)	96	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average
60	0.150	0.153	0.152
15	0.352	0.338	0.345
4.8	0.513	0.549	0.531
1.2	0.985	1.002	0.994
0.3	1.476	1.441	1.459
0	2.679	2.654	2.667

References

Chronic Administration of High Doses of NandroloneDecanoate on the Pituitary-Gonadal Axis in Male Rats Alsi J. et al,Basic Clin Pharmacol Toxicol,2009 Nov;105(5):307-14.
Erectogenic and neurotrophic effects of icariin, a purified extract of horny goat weed (Epimedium spp.) in vitro and in vivo /,The Journal of Sexual Medicine,2010 Apr;7(4 Pt 1):1518-28.
Extended intervention time and evaluation of sperm suppression by dienogest plus testosterone undecanoate in male rat /,Contraception,2012 Jan;85(1):113-21.
Clomiphene citrate potentiates the adverse effects of estrogen on rat testis and down-regulates the expression of steroidogenic enzyme genes Bharti S et al,Fertil Steril,2013 Jan;99(1):140-8.
A role for endocannabinoids in acute stress-induced suppression of the hypothalamic-pituitary-gonadal axis in male rats Karamikheirabad M et al,Clin Exp Reprod Med,2013 Dec;40(4):155-62.
Evaluation of effects of repeated sevoflurane exposure on rat testicular tissue and reproductive hormones Kaya Z et al,Inhal Toxicol.,2013 Mar;25(4):192-8.
Intermittent fasting dietary restriction regimen negatively influences reproduction in young rats: a study of hypothalamo-hypophysial-gonadal axis Kumar S et al,PLoS One,2013 8(1):e52416.
Impact of 900 MHz electromagnetic field exposure on main male reproductive hormone levels: a Rattus norvegicus model Sepehrimanesh M et al,Int J Biometeorol,2013 Dec 20.
Effects of the phytoestrogen genistein on the development of the reproductive system of Sprague Dawley rats Siti Rosmani Md Zin et al,Clinics,2013 68(2):253-62.
Introducing a rat model of prenatally androgen-induced polycystic ovary syndrome in adulthood Ramezani Tehrani F et al,Exp Physiol,2014 Feb 14.
A Survey of the Relationship Between Noised Pollution, Honey and Vitamin E and Plasma Level of Blood Sexual Hormones in Noise-Exposed Rats Asghar Rajabzadeh et al,Jentashapir Journal of Health Research,2015 Feb;6(1): e27331
Effect of Excess Iodine on Oxidative StressMarkers, Steroidogenic-Enzyme Activities, Testicular Morphology, and Functions in Adult Male Rats Chakraborty A. et al,Biol Trace Elem Res,2015 Dec 23.
Effect of honey on the reproductive system of male rat offspring exposed to prenatal restraint stress Haron MN. et al,Andrologia,2015 Aug 20.
Evaluation of offspring sex ratio, sex hormones and antioxidant enzymes following exposure to methyl tertiary butyl ether in adult male Sprague-Dawley rats. Khalili L.et al,EXCLI J.,14:75-82
Moxa smoke: Is it a beneficial or hazardous factor for infertility? A preclinical study on sperm parameters and sex hormones in male rats L Wang. et al,European Journal of Integrative Medicine,2016
Effects of low-dose cadmium exposure during gestation and lactation on development and reproduction in rats Luo X et al,Environ Sci Pollut Res Int,2015 Mar 5.
Honey and Vitamin E Restore the Plasma Level of Gonadal Hormones and Improve the Fertilization Capacity in Noise-Stressed Rats Rajabzadeh A.et al,A. General Policy,2015 2(2):64-68
The Effect of Intraventricular Administration of Zinc on Serum LH, FSH, Prolactin, and Testosterone in Male Rats Shahraki M R.et al,Zahedan Journal of Research in Medical Sciences,2015 17(9)
Increase in endogenous estradiol in the progeny of obese rats is associated with precocious puberty and altered follicular development in adulthood Ambrosetti V. et al,Endocrine,2016 Jan 14.
Active immunization with GnRH-tandem-dimer peptide in young male rats reduces serum reproductive hormone concentrations, testicular development and spermatogenesis Han X F.et al,Asian journal of andrology,,18(3): 485.
Disruptions in the hypothalamic-pituitary-gonadal axis in rat offspring following prenatal maternal exposure to lipopolysaccharide Izvolskaia MS.et al,Stress.,2016 1-8
THERAPEUTICS FOR THE INDUCTION OF ENDOGENOUS STEROIDOGENESIS AND METHODS ASSOCIATED WITH THEIR IDENTIFICATION Papadopoulos V.et al,U.S. Patent ,/
Protective Antioxidant Efficiency of Garlic against Lead-Induced Renal and Testicular Toxicity in Adult Male Rats Salem NA.et al,Heavy Metal Toxicity and Diseases,January
Surgical castration but not immuncastration is associated with reduced hypothalamic GnIH and GHRH/GH/IGF-I axis function in male rats Xingfa Han.et al,Theriogenology,2016
High-Cholesterol Diet Disrupts the Levels of Hormones Derived from Anterior Pituitary Basophilic Cells Yang J.et al,J Neuroendocrinol.,2016 ,28(3).
The anorectic agent, lorcaserin, disturbs estrous cyclicity and produces endometrial hyperplasia without affecting ovarian population in female rats Abdel Latif RT.et al,Life Sci.,Jun 6
Effect of prenatal DINCH plasticizer exposure on rat offspring testicular function and metabolism Campioli E.et al,Sci Rep,Sep 11
EFFECT OF ADDING DRIED GINGER RHIZOMES TO DIETS ON SEMEN QUALITY AND FERTILITY RATE IN AGED LOCAL COCKS UNDER EGYPTIAN HOT Ezzat, W.et al,Egyptian Poultry Science Journal,Mar
Citalopram Induces Reproductive Toxicity in Male Rats Ilgin S.et al,Birth Defects Res,Mar 31.
Alteration in follistatin gene expression detected in prenatally androgenized rats Marziyeh Salehi Jahromi.et al,Gynecol Endocrinol,Feb 26
Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress Merve Baysa.et al,PLOS ONE,April 18
Investigating the prenatal exposure of hydro-alcoholic extract of ginger on the function of Pituitary-Gonad axis in male mature offspring rats Nasim Zamani .et al,BASIC AND LABORATORY RESEARCH,/
Therapeutic effect of curcumin against nicotine-induced reproductive dysfunction in male rats Neveen A. Salem.et al,JIPBS,/
Lactational exposure of polychlorinated biphenyls impairs Leydig cellular steroidogenesis in F1 progeny rats Thangavelu SK.et al,Reprod Toxicol,Dec 2
Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats Ivana Jari.et al,Toxicology and Applied Pharmacology,Jan 15
Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats Tang YQ.et al,BMC Complement Altern Med,2 April
Protective effects of persian honey, Apis Mellifera Meda Skorikov on side effects of chemotherapy and ischemia/reperfusion induced testicular injury Mohammadreza Gholami .et al,Journal of Complementary and Integrative Medicine,May 23
Tetragonia tetragonioides (Pall.) Kuntze Regulates Androgen Production in a Letrozole-Induced Polycystic Ovary Syndrome Model Bo-Jeong Pyun.et al,Molecules,May 14
TOXIC EFFECTS OF TRAZADONE ON MALE REPRODUCTIVE SYSTEM VIA DISRUPTING HYPOTHALAMIC-PITUITARY-TESTICULAR AXIS AND INDUCING TESTICULAR OXIDATIVE STRESS Sinem Ilgin.et al,/,/
The Effect of Galega officinalis on Hormonal and Metabolic Profile in a Rat Model of Polycystic Ovary Syndrome Seyed Hosein Abtahi-Eivari.et al,International Journal of Women’s Health and Reproduction Sciences,July
Anti-hypercholesterolemic impacts of barley and date palm fruits on the ovary of Wistar albino rats and their offspring Hassan I.H.El-Sayyad.et al,Reproductive Biology,July 10
Amelioration of diabetes‐induced testicular and sperm damage in rats by cerium oxide nanoparticle treatment Tayebe Artimani.et al,ANDROLOGIA,July 18
Crataegus aronia enhances sperm parameters and preserves testicular architecture in both control and non-alcoholic fatty liver disease-induced rats Dallak M, et al,Pharmaceutical Biology,Octobert 30
Cyanidin-3-O-glucoside at Low Doses Protected against 3-Chloro-1, 2-propanediol Induced Testis Injury and Improved Spermatogenesis in Male Rats Jiang X, et al,Journal of Agricultural and Food Chemistry,Octobert 30
Protective role of Ficus deltoidea against BPA-induced impairments of the follicular development, estrous cycle, gonadotropin and sex steroid hormones level of prepubertal rats Zaid SSM, et al,Journal of ovarian research,November 26
Protective role of Ficus deltoidea against BPA-induced impairments of the follicular development, estrous cycle, gonadotropin and sex steroid hormones level of prepubertal rats Zaid SSM, et al,Journal of ovarian research,November 26

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Function	Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Subcellular Location	Secreted
Protein Families	Glycoprotein hormones subunit beta family
Database Links	KEGG: rno:25329 STRING: 10116.ENSRNOP00000060360 UniGene: Rn.10108

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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