Human Tumor necrosis factor α,TNF-α ELISA KIT

Code CSB-E04740h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01375
Lead Time 3-5 working days
Abbreviation TNF
Target Name tumor necrosis factor (TNF superfamily, member 2)
Alias DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
HGNC 11935
RGD 708418
MGI 88337
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 89-99  
1:2 Average % 90  
Range % 85-95  
1:4 Average % 97  
Range % 88-102  
1:8 Average % 92  
Range % 85-100  
Recovery
The recovery of human TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 94-101  
EDTA plasma (n=4) 96 88-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.607 2.705 2.656 2.482  
250 2.296 2.307 2.302 2.128  
125 1.642 1.624 1.633 1.459  
62.5 0.912 0.940 0.926 0.752  
31.2 0.489 0.504 0.497 0.323  
15.6 0.317 0.328 0.323 0.149  
7.8 0.282 0.271 0.277 0.103  
0 0.172 0.175 0.174    
References
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Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective
Involvement in disease Psoriatic arthritis (PSORAS)
Subcellular Location Cell membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, membrane form: Membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, soluble form: Secreted, SUBCELLULAR LOCATION: C-domain 1: Secreted, SUBCELLULAR LOCATION: C-domain 2: Secreted
Protein Families Tumor necrosis factor family
Database Links

HGNC: 11892

OMIM: 191160

KEGG: hsa:7124

STRING: 9606.ENSP00000398698

UniGene: Hs.241570

Pathway MAPK signaling pathway
mTOR signaling pathway
TGF-beta signaling pathway
NF-kappa B signaling pathway
TNF signaling pathway
Apoptosis
Necroptosis
Antigen processing and presentation
Fc epsilon RI signaling pathway
Hematopoietic cell lineage
IL-17 signaling pathway
Natural killer cell mediated cytotoxicity
NOD-like receptor signaling pathway
Osteoclast differentiation
T cell receptor signaling pathway
Toll-like receptor signaling pathway
Adipocytokine signaling pathway
AGE-RAGE signaling pathway in diabetic complications
Inflammatory Pain

Q&A and Customer Reviews

Customer Reviews
  • Sample type: Serum
    Sample species: Human
    Review: Cell viability and cytotoxicity of PBMCs (e), IL-6 (f) and TNF-α(g) secretion levels with different incubation time. Temperature was 37.5 °C, MNP@DMSA concentration was 1.0 mg/mL, and incubation time was 120 minutes.
    PMID: 30390582
  • Sample type: Serum
    Sample species: Human
    Review: Concentrations of IL-6 and TNF-α in MNP@CTAB-BSA group maintain at normal level when the incubation time increases from 30 minutes to 180 minutes. Concentrations of IL-6 and TNF-α in MNP@CTAB group rise above the normal level with prolonged incubation time.
  • Sample type: Cell culture supernatant
    Sample species: Human
    Review: Same trend was observed in the concentrations of IL-1α, IL-6, IL-8 and TNF-α in cell culture supernatant.
    PMID: 30026098
  • Sample type: Cell culture supernatant after Lnc-DC silencing
    Sample species: Human
    Review: The ELISA data indicate that Lnc-DC silencing significantly decreased the concentration of TNF-α .
    PMID: 30202418
  • Sample type: Serum
    Sample species: Human
    Review:The main outcome measures in this study were circulating pro-inflammatory cytokine levels (TNF-α, IL-1B, IL-6, IL-10), urinary cortisol levels, beck depression inventory (BDI) score, weight and body mass index (BMI).
  • Sample type: Serum
    Sample species: Human
    Review: ELISA was used to analyze cytokines (TNF-α, IFN-γ, IL-2 and IL-6) expression after Jurkat and P116 cells were stimulated with anti-CD3/CD28 antibodies for 24 hours.
    PMID: 30639475

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