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Rat TNF-α ELISA kit

Citations (455) Protocol MSDS
Code CSB-E11987r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
tumor necrosis factor (TNF superfamily, member 2)
Alternative Names
Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
Abbreviation
TNF
Uniprot No.
P16599
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 90  
Range % 87-96  
1:2 Average % 99  
Range % 95-104  
1:4 Average % 101  
Range % 97-105  
1:8 Average % 98  
Range % 93-107  
Recovery
The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 85 80-92  
EDTA plasma (n=4) 99 95-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.037 2.067 2.052 1.871  
200 1.445 1.489 1.467 1.286  
100 1.017 1.034 1.026 0.845  
50 0.655 0.677 0.666 0.485  
25 0.411 0.438 0.425 0.244  
12.5 0.351 0.378 0.365 0.184  
6.25 0.297 0.282 0.290 0.109  
0 0.183 0.179 0.181 　  
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat TNF ELISA Kit was designed for the quantitative measurement of Rat TNF protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

Citations

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Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
4.6 - 10 reviews

Submit a Review here

Sample type: Tissue homogenate(PVN tissue)

Sample species: Rat

Review: The cytokine levels of IL-1β(F) and TNF-α(G) from the PVN tissue as measured by ELISA. n = 10–11 for each group. *P < 0.01 and **P < 0.05 versus sham groups; &P < 0.01 and #P < 0.05 versus MI + shCtrl group.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Rat

Review: The TNF-α protein level in the conditioned media.#P < 0.05 vs Control group; *P < 0.05 vs Ang II group. The statistical analyses were performed using one-way ANOVA followed by Bonferroni\'s post hoc test. n = 3 independent experiments.

By Anonymous

Sample type: Tissue homogenate(lung tissue)

Sample species: Rat

Review: MSCs-HO-1 decreased LPS-mediated tumor necrosis factor α (TNF-α; a) and interleukin 1β (IL-1β; b) in sera of all groups at the indicated time points. (c, d) MSCs-HO-1 decreased LPS-induced TNF-α (c) and IL-1β (d) in the lungs of all groups at the indicated time points. All data are expressed as mean ± SEM (n = 6). *p < 0.05 versus 0 day time point, and #p < 0.05 versus lenti-HO-1-GFP and MSC groups at each time point.

By Anonymous

Sample type: Serum

Sample species: Rat

Review: Effect of GABARAP and DCs on cartilage appearance and the expression of inflammatory cytokines in serum. In vivo cartilagerepair at 4 and 8 weeks postsurgery, (a) Macroscopic appearance and (b) macroscopic scores of femoral condyles were detected. Meanwhile, serum levels of (c) IL-6, (d) TNF-α, and (e) MMP-13 were determined using ELISA kits. Values are the means ± SD (n = 12). Bars with different letters are significantly different from each other at p < 0.05 and those with the same letter exhibit no significant difference. DC: differentiated chondrocytes; ELISA: enzyme-linked immunosorbent assay; GABARAP: γ-aminobutyric acid receptor-associated protein; SD: standard deviation 9Color figure can be viewed at wileyonlinelibrary.com).

By Anonymous

Sample type: Tissue homogenate(Colorectal tissues)

Sample species: Rat

Review: Effects of BD-SNEDDS on colon levels of inflammatory cytokines and oxidative stress.

By Anonymous

Sample type: Tissue homogenate(Liver homogenate)

Sample species: Rat

Review: Effects of MFKE on proinflammatory biomarkers in rats treated with APAP-induced liver toxicity. (A) MRNA expressions of tumor necrosis factor-alpha, interleukin-1, and inducible nitric oxide synthase.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Rat

Review: The release of TNF-α. Single asterisk refers to p < 0.05 and double asterisks refer to p < 0.01.

By Anonymous

Sample type: Tissue homogenate(Spinal cord tissue)

Sample species: Rat

Review: The concentrations of inflammatory cytokines IL-6 (a), TNF-α (b), and IL-1β (c) and anti‐inflammatory cytokines TGF-β (d) and IL‐13 (e) were measured by ELISA in sham-operated rats (n = 6) and in SCI rats treated with melatonin (n = 6) or vehicle (n = 6). Data are presented as mean ± SEM, ***p < 0.001: SCI + Vehicle versus sham; ### p < 0.001: SCI + Melatonin versus SCI + Vehicle, and One‐way ANOVA.

By Anonymous

Sample type: Biological fluids(Bronchoalveolar lavage fluid)

Sample species: Rat

Review: Levels of TNF-α, IL-6, IL-8, and IL-10 detected by ELISA. n =15 in each group; The measurement data were expressed by mean ± standard deviation, one-way ANOVA was used for comparison between groups and the survival rate of 0~120 h in each group was compared by Kaplan-Meier analysis; *, p < 0.05 when compared with the normal group; #, p <0.05 when compared with the ALI + PBS group; eMSCs, mesenchymal stem cell; ALI, acute lung injury; OS, oxidative stress; TNF, tumor necrosis factor; IL, interleukin; SOD, superoxide dismutase; MDA, methylenedioxyamphetamine; GSH, glutathione; BALF, bronchoalveolar lavage fluid; ELISA, enzyme-linked immunosorbent assay; HE, hematoxylin-eosin; ANOVA, analysis of variance.

By Anonymous

Sample type: Tissue homogenate(heart tissue homogenate)

Sample species: Rat

Review: The standard was worked perfectly in my experiment but I could not get much difference in control and disease group animal serum samples.

By Anonymous

Read more reviews >>
■ Q&A
Q:

Seeking for ELISA kits of TNF a .I am working on SD Rats and project related to High Fat Diet induced Metabolic complications.

A:
Thanks for your inquiry.
For the mentiond detection targets,we have the following kit:
#CSB-E11987r,Rat TNF-α ELISA kit
And we use kits to test ordinary rats.While there is not big problems to detect SD Rats.Pls take your actual test results as the real results
Q:

Rat TNF-α ELISA kit -CSB-E11987r this kit can be used for estimation in BALF...fluid
Kindly answer me.

A:
Thanks for your enquiry.
We usually use this kit to test serum, plasma, cell culture supernates, tissue homogenates.If you want to test bronchoalveolar lavage fluid,we suggest you do pre-testing first.If the content of your sample falls in the detection range,it can usually be detected out.
The manual will be provided to you by e-mail.
Pls let me know if you have any further questions. Thank you.
Q:

I want to know what the normal serum concentrations of TNFa in experimental rats are and the serum concentrations of TNFa in an induced freund adjuvant RA in experimental rats during 7 weeks. And I want to know the concentrations estimated in nano gramml. I want to ask about the weight loss of experimental animal rats also during an induced freund adjuvant arthritis RA during 7 weeks estimated by the gram.

A:

We used this CSB-E11987r Rat TNF-α ELISA kit to measure normal serum/plasma samples on rats. The model with heavy inflammation could even reach hundreds of pg/ml. The results varied with different conditions of the sample and different organism models. As to the mentioned induced model, we are not so sure about the sample condition. We suggest taking the actual measurement as your real result.
As to your question about weight loss, it usually depends on your own experiment aim or the choice of your target. If you just want to test TNF-α, then it is just ok to use the form of concentration of TNF-α.

Target Background

Function
(From Uniprot)
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Subcellular Location
Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
Protein Families
Tumor necrosis factor family
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