Rat TNF-α ELISA kit

Code CSB-E11987r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P16599
Lead Time 3-5 working days
Abbreviation TNF
Target Name tumor necrosis factor (TNF superfamily, member 2)
Alias DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 87-96  
1:2 Average % 99  
Range % 95-104  
1:4 Average % 101  
Range % 97-105  
1:8 Average % 98  
Range % 93-107  
Recovery
The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 85 80-92  
EDTA plasma (n=4) 99 95-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.037 2.067 2.052 1.871  
200 1.445 1.489 1.467 1.286  
100 1.017 1.034 1.026 0.845  
50 0.655 0.677 0.666 0.485  
25 0.411 0.438 0.425 0.244  
12.5 0.351 0.378 0.365 0.184  
6.25 0.297 0.282 0.290 0.109  
0 0.183 0.179 0.181    
References
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Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.; FUNCTION
Subcellular Location Cell membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, membrane form: Membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, soluble form: Secreted, SUBCELLULAR LOCATION: C-domain 1: Secreted, SUBCELLULAR LOCATION: C-domain 2: Secreted
Protein Families Tumor necrosis factor family
Database Links

KEGG: rno:103694380

STRING: 10116.ENSRNOP00000001110

UniGene: Rn.2275

Pathway MAPK signaling pathway
mTOR signaling pathway
TGF-beta signaling pathway
NF-kappa B signaling pathway
TNF signaling pathway
Apoptosis
Necroptosis
Antigen processing and presentation
Fc epsilon RI signaling pathway
Hematopoietic cell lineage
IL-17 signaling pathway
Natural killer cell mediated cytotoxicity
NOD-like receptor signaling pathway
Osteoclast differentiation
T cell receptor signaling pathway
Toll-like receptor signaling pathway
Adipocytokine signaling pathway
AGE-RAGE signaling pathway in diabetic complications
Inflammatory Pain

Q&A and Customer Reviews

Q&A
Q:

Seeking for ELISA kits of TNF a .I am working on SD Rats and project related to High Fat Diet induced Metabolic complications.

A:
Thanks for your inquiry.
For the mentiond detection targets,we have the following kit:
#CSB-E11987r,Rat TNF-α ELISA kit
And we use kits to test ordinary rats.While there is not big problems to detect SD Rats.Pls take your actual test results as the real results
Q:

Rat TNF-α ELISA kit -CSB-E11987r this kit can be used for estimation in BALF...fluid
Kindly answer me.

A:
Thanks for your enquiry.
We usually use this kit to test serum, plasma, cell culture supernates, tissue homogenates.If you want to test bronchoalveolar lavage fluid,we suggest you do pre-testing first.If the content of your sample falls in the detection range,it can usually be detected out.
The manual will be provided to you by e-mail.
Pls let me know if you have any further questions. Thank you.
Customer Reviews
  • Sample type: PVN tissue
    Sample species: Rat
    Review: The cytokine levels of IL-1β(F) and TNF-α(G) from the PVN tissue as measured by ELISA. n = 10–11 for each group. *P < 0.01 and **P < 0.05 versus sham groups; &P < 0.01 and #P < 0.05 versus MI + shCtrl group.
    PMID: 30978539
  • Sample type: Myocardial cells
    Sample species: Rat
    Review: TNF-α protein level in the conditioned media.#P < 0.05 vs Control group; *P < 0.05 vs Ang II group. The statistical analyses were performed using one-way ANOVA followed by Bonferroni's post hoc test. n = 3 independent experiments.
    PMID: 30953402
  • Sample type: Serum and lung tissue
    Sample species:Rat
    Review: MSCs-HO-1 decreased LPS-mediated tumor necrosis factor α (TNF-α; a) and interleukin 1β (IL-1β; b) in sera of all groups at the indicated time points. (c, d) MSCs-HO-1 decreased LPS-induced TNF-α (c) and IL-1β (d) in lungs of all groups at the indicated time points. All data are expressed as mean ± SEM (n = 6). *p < 0.05 versus 0 day time point, and #p < 0.05 versus lenti-HO-1-GFP and MSC groups at each time point.
    PMID: 30362554
  • Sample type: Serum
    Sample species: Rat
    Review: Effect of GABARAP and DCs on cartilage appearance and the expression of inflammatory cytokines in serum. In vivo cartilagerepair at 4 and 8 weeks postsurgery, (a) Macroscopic appearance and (b) macroscopic scores of femoral condyles were detected. Meanwhile,serum levels of (c) IL-6, (d) TNF-α, and (e) MMP-13 were determined using ELISA kits. Values are the means ± SD (n = 12). Bars with different letters are significantly different from each other at p < 0.05 and those with the same letter exhibit no significant difference. DC: differentiated chondrocytes; ELISA: enzyme-linked immunosorbent assay; GABARAP: γ-aminobutyric acid receptor-associated protein; SD: standard deviation [Color figure can be viewed at wileyonlinelibrary.com].
    PMID: 31020644
  • Sample type: Colorectal tissues
    Sample species: Rat
    Review: Effects of BD-SNEDDS on colon levels of inflammatory cytokines and oxidative stress.
    PMID: 30319255
  • Sample type: Liver homogenate
    Sample species: Rat
    Review: Effects of MFKE on proinflammatory biomarkers in rats treated with APAP-induced liver toxicity. (A) MRNA expressions of tumour necrosis factor- , interlukin-1 and inducible nitric oxide synthase.
    PMID: 30823534
  • Sample type: Serum
    Sample species: Rat
    Review: Serum concentrations of biomarkers, including TGF-β1, TNFα, IL-1β, IL-6, IL-18, CRP, and BNP were measured by Elisa kits following manufacture instructions.
    PMID: 30466624
  • Sample type: Cell culture supernates
    Sample species: Rat
    Review:The release of TNF-α. Single asterisk refers to p < 0.05 and double asterisks refer to p < 0.01.
    PMID: 30675709
  • Sample type: Spinal cord tissue
    Sample species: Rat
    Review: The concentrations of inflammatory cytokines IL-6 (a), TNF-α (b), and IL-1β (c) and anti‐inflammatory cytokines TGF-β (d) and IL‐13 (e) were measured by ELISA in sham-operated rats (n = 6) and in SCI rats treated with melatonin (n = 6) or vehicle (n = 6). Data are presented as mean ± SEM, ***p < 0.001: SCI + Vehicle versus sham; ### p < 0.001: SCI + Melatonin versus SCI + Vehicle, and One‐way ANOVA.
    PMID: 31006904
  • Sample type: Bronchoalveolar lavage fluid
    Sample species: Rat
    Review: Levels of TNF-α, IL-6, IL-8, and IL-10 detected by ELISA. n =15 in each group; The measurement data were expressed by mean ± standard deviation, one-way ANOVA was used for comparison between groups and the survival rate of 0~120 h in each group was compared by Kaplan-Meier analysis; *, p < 0.05 when compared with the normal group; #, p <0.05 when compared with the ALI + PBS group; eMSCs, mesenchymal stem cell; ALI, acute lung injury; OS, oxidative stress; TNF, tumor necrosis factor; IL, interleukin; SOD, superoxide dismutase; MDA, methylenedioxyamphetamine; GSH, glutathione; BALF, bronchoalveolar lavage fluid; ELISA, enzyme linked immunosorbent assay; HE, hematoxylin-eosin; ANOVA, analysis of variance.
    PMID: 30892077
  • Tested application: ELISA
    Test sample: Serum,Tissue homogenate: heart tissue homogenate
    Species: Rat
    Review: The standard was worked perfectly in my experiment but I could don get much difference in control and disease group animal serum samples.

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