Code | CSB-E04638h |
Size | 96T,5×96T,10×96T |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
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Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The human IL-6 ELISA kit is suitable for the quantitative determination of human IL-6 in a wide range of sample types, including serum, plasma, cell culture supernates, tissue homogenates, and urine. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products are positively related to the analyte of interest in the sample.This human IL-6 ELISA kit has been described in up to 143 publications.
IL-6 is rapidly and transiently generated in response to infections and tissue damage, contributing to host defense via the induction of acute-phase responses, hematopoiesis, and immune reactions. In addition to regulating immune and inflammatory reactions, IL-6 also plays an important role in hematopoiesis, metabolism, and organ development. IL-6 binding to its receptor IL-6R ligates to gp130, initiating a downstream signal cascade, including JAK/STAT3 and JAK-SHP-2-MAPK pathways. Dysregulated IL-6 has been associated with the pathogenesis of autoimmune and inflammatory diseases, metabolic abnormalities, and cancer. Targeting IL-6 has shown to be potential for the treatment of various immune-mediated diseases.
Sample type: Serum
Sample species: Human
Review: Cell viability and cytotoxicity of PBMCs (e), IL-6 (f) and TNF-α(g) secretion levels with different incubation time. Temperature was 37.5 °C, MNP@DMSA concentration was 1.0 mg mL-1, and incubation time was 120 minutes.
By Anonymous
Sample type: Serum
Sample species: Human
Review: Serum β-EP, MOR, IL-6 and IL-10 concentrations in the MDD were significantly higher than in the control group (p<0.05, Table 2), while EM-2 was not significantly different between both groups.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Human
Review: The ELISA data indicate that Lnc-DC silencing significantly decreased the concentration of TNF-α. Similarly, we also observed a reduction in the concentration of IL-6.
By Anonymous
Sample type: Serum
Sample species: Human
Review: ELISA was used to analyze cytokines (TNF-α, IFN-γ, IL-2 and IL-6) expression after Jurkat and P116 cells were stimulated with anti-CD3/CD28 antibodies for 24 hours.
By Anonymous