| Code | CSB-E04638h |
| Size | 96T,5×96T,10×96T |
| Trial Size |  |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
The human IL-6 ELISA kit is suitable for the quantitative determination of human IL-6 in a wide range of sample types, including serum, plasma, cell culture supernates, tissue homogenates, and urine. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products are positively related to the analyte of interest in the sample.This human IL-6 ELISA kit has been described in up to 143 publications. IL-6 is rapidly and transiently generated in response to infections and tissue damage, contributing to host defense via the induction of acute-phase responses, hematopoiesis, and immune reactions. In addition to regulating immune and inflammatory reactions, IL-6 also plays an important role in hematopoiesis, metabolism, and organ development. IL-6 binding to its receptor IL-6R ligates to gp130, initiating a downstream signal cascade, including JAK/STAT3 and JAK-SHP-2-MAPK pathways. Dysregulated IL-6 has been associated with the pathogenesis of autoimmune and inflammatory diseases, metabolic abnormalities, and cancer. Targeting IL-6 has shown to be potential for the treatment of various immune-mediated diseases. |
| Target Name | interleukin 6 (interferon, beta 2) |
| Alternative Names | Interleukin BSF 2 ELISA Kit; B cell differentiation factor ELISA Kit; B cell stimulatory factor 2 ELISA Kit; B-cell stimulatory factor 2 ELISA Kit; BSF 2 ELISA Kit; BSF-2 ELISA Kit; BSF2 ELISA Kit; CDF ELISA Kit; CTL differentiation factor ELISA Kit; Cytotoxic T cell differentiation factor ELISA Kit; Hepatocyte stimulating factor ELISA Kit; Hepatocyte stimulatory factor ELISA Kit; HGF ELISA Kit; HSF ELISA Kit; Hybridoma growth factor ELISA Kit; Hybridoma growth factor Interferon beta-2 ELISA Kit; Hybridoma plasmacytoma growth factor ELISA Kit; IFN-beta-2 ELISA Kit; IFNB2 ELISA Kit; IL 6 ELISA Kit; IL-6 ELISA Kit; IL6 ELISA Kit; IL6_HUMAN ELISA Kit; Interferon beta 2 ELISA Kit; Interferon beta-2 ELISA Kit; Interleukin 6 ELISA Kit; Interleukin 6 (interferon beta 2) ELISA Kit; Interleukin BSF 2 ELISA Kit; Interleukin-6 ELISA Kit |
| Abbreviation | IL6 |
| Uniprot No. | P05231 |
| Species | Homo sapiens (Human) |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates, urine |
| Detection Range | 7.8 pg/mL-500 pg/mL |
| Sensitivity | 2.453 pg/mL |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision |
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays):CV%<10% Three samples of known concentration were tested in twenty assays to assess.
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| Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
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| Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
| Troubleshooting and FAQs |
ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
Sample type: Serum
Sample species: Human
Review: Cell viability and cytotoxicity of PBMCs (e), IL-6 (f) and TNF-α(g) secretion levels with different incubation time. Temperature was 37.5 °C, [email protected] concentration was 1.0 mg mL-1, and incubation time was 120 minutes.
By Anonymous
Sample type: Serum
Sample species: Human
Review: Serum β-EP, MOR, IL-6 and IL-10 concentrations in the MDD were significantly higher than in the control group (p<0.05, Table 2), while EM-2 was not significantly different between both groups.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Human
Review: The ELISA data indicate that Lnc-DC silencing significantly decreased the concentration of TNF-α. Similarly, we also observed a reduction in the concentration of IL-6.
By Anonymous
Sample type: Serum
Sample species: Human
Review: ELISA was used to analyze cytokines (TNF-α, IFN-γ, IL-2 and IL-6) expression after Jurkat and P116 cells were stimulated with anti-CD3/CD28 antibodies for 24 hours.
By Anonymous
| Function (From Uniprot) |
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury (Probable). In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells.; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine (Probable). Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.
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| Gene References into Functions |
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| Involvement in disease | Rheumatoid arthritis systemic juvenile (RASJ) |
| Subcellular Location | Secreted. |
| Protein Families | IL-6 superfamily |
| Tissue Specificity | Produced by skeletal muscle. |
| Database Links |
HGNC: 6018 OMIM: 147620 KEGG: hsa:3569 STRING: 9606.ENSP00000258743 UniGene: Hs.654458 |
