Human Interleukin 6,IL-6 ELISA KIT

Code CSB-E04638h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P05231
Lead Time 3-5 working days
Abbreviation IL6
Protein Biological Process 1 Cytokine
Target Name interleukin 6 (interferon, beta 2)
Alias BSF2, HGF, HSF, IFNB2, IL-6, B cell stimulatory factor-2|B-cell differentiation factor|CTL differentiation factor|OTTHUMP00000158544|hybridoma growth factor|interleukin 6|interleukin BSF-2
Species Homo sapiens (Human)
Protein Biological Process 3 Acute phase
Sample Types serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 2.453 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.
HGNC 1834
RGD 2327
MGI 88373
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

 

 

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

References
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Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.
Involvement in disease Rheumatoid arthritis systemic juvenile (RASJ)
Subcellular Location Secreted
Protein Families IL-6 superfamily
Database Links

HGNC: 6018

OMIM: 147620

KEGG: hsa:3569

STRING: 9606.ENSP00000258743

UniGene: Hs.654458

Pathway HIF-1 signaling pathway
Jak-STAT signaling pathway
PI3K-Akt signaling pathway
TNF signaling pathway
Cellular senescence
Hematopoietic cell lineage
IL-17 signaling pathway
NOD-like receptor signaling pathway
Th17 cell differentiation
Toll-like receptor signaling pathway
AGE-RAGE signaling pathway in diabetic complications
FoxO signaling pathway
Inflammatory Pain

Q&A and Customer Reviews

Customer Reviews
  • Sample type: Serum
    Sample species: Human
    Review: Cell viability and cytotoxicity of PBMCs (e), IL-6 (f) and TNF-α(g) secretion levels with different incubation time. Temperature was 37.5 °C, MNP@DMSA concentration was 1.0 mg mL-1, and incubation time was 120 minutes.
    PMID: 30390582
  • Sample type: Serum
    Sample species: Human
    Review: Concentrations of IL-6 and TNF-α in MNP@CTAB-BSA group maintain at normal level when the incubation time increases from 30 minutes to 180 minutes. Concentrations of IL-6 and TNF-α in MNP@CTAB group rise above the normal level with prolonged incubation time.
  • Sample type: Serum
    Sample species: Human
    Review: Serum β-EP, MOR, IL-6 and IL-10 concentrations in the MDD were significantly higher than in the control group (p<0.05, Table 2), while EM-2 was not significantly different between both groups.
    PMID: 30677547
  • Sample type: Plasma (ARDS and Non-ARDS Groups)
    Sample species: Human
    Review: We found that plasma ANG-2 and sE-selectin levels in ARDS patients were dramatically higher than those in nonARDS patients, but IL-8 or IL-6 level displayed no difference between the ARDS and non-ARDS groups.
    PMID: 30008611
  • Sample type: Cell culture supernatant after Lnc-DC silencing
    Sample species: Human
    Review: The ELISA data indicate that Lnc-DC silencing significantly decreased the concentration of TNF-α. Similarly, we also observed a reduction in the concentration of IL-6
    PMID: 30202418
  • Tested application: ELISA
    Test sample: Biological fluids (Milk)
    Species: Human
    Review: We tested milk samples from healthy donors across lactational stage, so from 1 week after birth to 6 months, samples were collected every 2-4 weeks. Plated in duplicate for ELISA. No differences observed over time.
  • Sample type: Serum
    Sample species: Human
    Review:The main outcome measures in this study were circulating pro-inflammatory cytokine levels (TNF-α, IL-1B, IL-6, IL-10), urinary cortisol levels, beck depression inventory (BDI) score, weight and body mass index (BMI).
  • Sample type: Serum
    Sample species: Human
    Review: ELISA was used to analyze cytokines (TNF-α, IFN-γ, IL-2 and IL-6) expression after Jurkat and P116 cells were stimulated with anti-CD3/CD28 antibodiesfor 24 hours.
    PMID: 30639475

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