Code | CSB-E04638h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
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Trial Size |
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Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The human IL-6 ELISA kit is suitable for the quantitative determination of human IL-6 in a wide range of sample types, including serum, plasma, cell culture supernates, tissue homogenates, and urine. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products are positively related to the analyte of interest in the sample.This human IL-6 ELISA kit has been described in up to 143 publications.
IL-6 is rapidly and transiently generated in response to infections and tissue damage, contributing to host defense via the induction of acute-phase responses, hematopoiesis, and immune reactions. In addition to regulating immune and inflammatory reactions, IL-6 also plays an important role in hematopoiesis, metabolism, and organ development. IL-6 binding to its receptor IL-6R ligates to gp130, initiating a downstream signal cascade, including JAK/STAT3 and JAK-SHP-2-MAPK pathways. Dysregulated IL-6 has been associated with the pathogenesis of autoimmune and inflammatory diseases, metabolic abnormalities, and cancer. Targeting IL-6 has shown to be potential for the treatment of various immune-mediated diseases.
Sample type: Serum
Sample species: Human
Review: Cell viability and cytotoxicity of PBMCs (e), IL-6 (f) and TNF-α(g) secretion levels with different incubation time. Temperature was 37.5 °C, MNP@DMSA concentration was 1.0 mg mL-1, and incubation time was 120 minutes.
By Anonymous
Sample type: Serum
Sample species: Human
Review: Serum β-EP, MOR, IL-6 and IL-10 concentrations in the MDD were significantly higher than in the control group (p<0.05, Table 2), while EM-2 was not significantly different between both groups.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Human
Review: The ELISA data indicate that Lnc-DC silencing significantly decreased the concentration of TNF-α. Similarly, we also observed a reduction in the concentration of IL-6.
By Anonymous
Sample type: Serum
Sample species: Human
Review: ELISA was used to analyze cytokines (TNF-α, IFN-γ, IL-2 and IL-6) expression after Jurkat and P116 cells were stimulated with anti-CD3/CD28 antibodies for 24 hours.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Human
Sample dilution: 1:20
Review: This brand of kit has been used for many years. It is necessary to do a pre-experiment in advance to feel the best dilution concentration, and do not blindly do the formal experiment. The OD values are within the range of standard curve, and the results are the same as expected. The cell supernatant of LPS model group was diluted 1:20, and the OD of diluted samples was 0.393-1.550.
By Anonymous