Recombinant Human SARS coronavirus Spike glycoprotein(S),partial (Active)

In Stock
Code CSB-MP348663HQE
Size $4660Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2 (CSB-MP684964PAL), the EC50 is 5.056-7.559 ng/ml. Biological Activity Assay
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 7.941-10.49 ng/ml. Biological Activity Assay
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Product Details

Description

Human SARS-CoV spike (S) protein amino acids 306-527 linked an N-terminal 10xHis-tag by the Thrombin linker, as well as a C-terminal Myc tag, was expressed in mammalian cells. The product is the recombinant human SARS-CoV S protein. This SARS-CoV S protein is an active protein, whose activity has been determined through functional ELISA by binding to ACE2 of Paguma larvata and human, with EC50 values of 5.056-7.559 ng/ mL and 7.941-10.49 ng/ mL, respectively. Its purity is high (>95%, SDS-PAGE) and endotoxin is low (<1.0 EU/ug protein, LAL method). Due to the glycosylation, it has an apparent molecular weight of 36 kDa on the gel. It is in stock now.

The SARS-CoV is an enveloped, single, and positive-stranded RNA virus. Its S protein is made up of the S1 subunit and S2 subunit. The S1 subunit contains a receptor-binding domain responsible for the engagement with the host cell receptor ACE2. The S2 subunit mediates the virus-host cell membrane fusion. The S protein plays important role in the induction of neutralizing-antibody and T-cell responses, and protective immunity during SARS-CoV infection.

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/ug as determined by LAL method.
Activity ①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2 (CSB-MP684964PAL), the EC50 is 5.056-7.559 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 7.941-10.49 ng/ml.
Target Names S
Uniprot No. P59594
Research Area Microbiology
Alternative Names (S glycoprotein)(E2)(Peplomer protein)(Spike protein S1)(Spike protein S2)
Molecular Characterization
Species Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Source Mammalian cell
Expression Region 306-527aa
Target Protein Sequence RVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNF
Mol. Weight 30 kDa
Protein Length Partial
Tag Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
(From Uniprot)
S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.; FUNCTION
Subcellular Location Virion membrane, Single-pass type I membrane protein, Host endoplasmic reticulum-Golgi intermediate compartment membrane, Single-pass type I membrane protein, Host cell membrane, Single-pass type I membrane protein
Protein Families Betacoronaviruses spike protein family

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