BUB3

BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog) is a core component of the spindle assembly checkpoint (SAC), with aliases like BUB3_HUMAN. It forms a complex with BUB1 to recruit Mad2 to kinetochores, monitoring proper spindle-microtubule attachment to centromeres. This ensures accurate chromosome segregation during mitosis, preventing aneuploidy.
Aberrant BUB3 expression links to cancers (e.g., colorectal, breast) via disrupted SAC function and chromosomal instability. While direct BUB3-targeted drugs remain preclinical, therapies targeting its interacting partners (e.g., BUB1) or SAC pathways are explored for anti-tumor efficacy, leveraging its role in maintaining genomic integrity.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

BUB3 Antibody (CSB-PA002884LA01HU)

Validated Data

Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, Mouse brain tissue
All lanes: BUB3 antibody at 3.4μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38, 37 kDa
Observed band size: 38, 37 kDa

Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA002884LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA002884LA01HU at dilution of 1:100

The following BUB3 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

BUB3 Antibodies

BUB3 Proteins

BUB3 Antibodies

BUB3 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA002884GA01HU	BUB3 Antibody	Human,Mouse,Rat	ELISA,WB,IF
CSB-PA117544	Phospho-BUB3 (Tyr207) Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA001070	BUB3 Antibody	Human,Mouse,Rat	WB, IHC, IF, ELISA
CSB-PA912911	BUB3 Antibody	Human,Mouse	ELISA,WB
CSB-PA002884LA01HU	BUB3 Antibody	Human, Mouse	ELISA, WB, IHC
CSB-PA002884LB01HU	BUB3 Antibody, HRP conjugated	Human	ELISA
CSB-RA549049A0HU	BUB3 Recombinant Monoclonal Antibody	Human	ELISA, IHC, IF, FC

BUB3 Antibodies for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Code	Product Name	Species Reactivity	Application
CSB-PA326630XA01SVG	BUB3 Antibody	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	ELISA, WB (ensure identification of antigen)

BUB3 Antibodies for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Code	Product Name	Species Reactivity	Application
CSB-PA002884XA01SXV	bub3 Antibody	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)	ELISA, WB (ensure identification of antigen)

BUB3 Proteins

BUB3 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP002884HU CSB-EP002884HU CSB-BP002884HU CSB-MP002884HU CSB-EP002884HU-B	Recombinant Human Mitotic checkpoint protein BUB3 (BUB3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP002884HUb1	Recombinant Human Mitotic checkpoint protein BUB3 (BUB3)	E.coli
CSB-EP002884HUj9	Recombinant Human Mitotic checkpoint protein BUB3 (BUB3)	E.coli
CSB-BP002884HUj9	Recombinant Human Mitotic checkpoint protein BUB3 (BUB3）	Homo sapiens (Human)

BUB3 Proteins for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Code	Product Name	Source
CSB-YP002884SXV CSB-EP002884SXV CSB-BP002884SXV CSB-MP002884SXV CSB-EP002884SXV-B	Recombinant Schizosaccharomyces pombe Mitotic checkpoint protein bub3 (bub3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

BUB3 Proteins for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Code	Product Name	Source
CSB-YP326630SVG CSB-EP326630SVG CSB-BP326630SVG CSB-MP326630SVG CSB-EP326630SVG-B	Recombinant Saccharomyces cerevisiae Cell cycle arrest protein BUB3 (BUB3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

BUB3 Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP628839BO CSB-EP628839BO CSB-BP628839BO CSB-MP628839BO CSB-EP628839BO-B	Recombinant Bovine Mitotic checkpoint protein BUB3 (BUB3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

BUB3 Proteins for Dictyostelium discoideum (Slime mold)

Code	Product Name	Source
CSB-YP709659DKK CSB-EP709659DKK CSB-BP709659DKK CSB-MP709659DKK CSB-EP709659DKK-B	Recombinant Dictyostelium discoideum Mitotic checkpoint protein bub3 (bub3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

BUB3 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)

Code	Product Name	Source
CSB-YP732732PYX CSB-EP732732PYX CSB-BP732732PYX CSB-MP732732PYX CSB-EP732732PYX-B	Recombinant Pongo abelii Mitotic checkpoint protein BUB3 (BUB3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

BUB3 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP893001MO CSB-EP893001MO CSB-BP893001MO CSB-MP893001MO CSB-EP893001MO-B	Recombinant Mouse Mitotic checkpoint protein BUB3 (Bub3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

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BUB3

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