CD47 (also known as Integrin-Associated Protein or IAP) is a transmembrane protein composed of five extracellular cytoplasmic domains and a short cytoplasmic tail. Its extracellular domains interact with other proteins, while its intracellular tail is involved in intracellular signal transduction. It is widely distributed on the cell surface and plays a significant role in various biological processes. CD47 serves multiple biological functions, including self-recognition, regulation of immune responses, recognition of blood cells, and tumor immune evasion. Research on CD47 is providing new insights and therapeutic strategies in the fields of immune system regulation and cancer treatment.
More Interesting read related CD47, A New Target for Tumor Immune
● Recombinant Human Leukocyte surface antigen CD47(CD47),partial (Active) CSB-MP004940HU
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity: Measured by its binding ability in a functional ELISA. Immobilized SIRPA (CSB-MP021334HU) at 2 μg/ml can bind human CD47, the EC50 of human CD47 protein is 65.91-82.42 ng/ml.
Human SIRPA protein His/Myc tag (CSB-MP021334HU) captured on COOH chip can bind Human CD47 protein Fc tag (CSB-MP004940HU) with an affinity constant of 19.1 nM as detected by LSPR Assay.
● CD47 Recombinant Monoclonal Antibody CSB-RA802124A0HU
Validated Data
Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate
All lanes: CD47 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36, 32, 34 kDa
Observed band size: 55 kDa
IHC image of CSB-RA802124A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of MCF7 Cells with CSB-RA802124A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
The following CD47 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CD47 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004940GA01HU | CD47 Antibody |
Human | ELISA,WB |
CSB-PA005993 | CD47 Antibody |
Human | WB, ELISA |
CSB-PA445679 | CD47 Antibody |
Human | ELISA,WB,IHC |
CSB-PA911004 | CD47 Antibody |
Human | ELISA,IHC |
CSB-PA004940LA01HU | CD47 Antibody |
Human | ELISA, IHC, IF |
CSB-PA004940LB01HU | CD47 Antibody, HRP conjugated |
Human | ELISA |
CSB-PA004940LC01HU | CD47 Antibody, FITC conjugated |
Human | |
CSB-PA004940LD01HU | CD47 Antibody, Biotin conjugated |
Human | ELISA |
CSB-RA802124A0HU | CD47 Recombinant Monoclonal Antibody |
Human | ELISA, WB, IHC, IF |
CD47 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-CF713985MO | Recombinant Mouse Leukocyte surface antigen CD47 (Cd47) |
in vitro E.coli expression system |
CSB-YP713985MO1 CSB-EP713985MO1 CSB-BP713985MO1 CSB-MP713985MO1 CSB-EP713985MO1-B |
Recombinant Mouse Leukocyte surface antigen CD47 (Cd47), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-AP005351MO | Recombinant Mouse Leukocyte surface antigen CD47 (Cd47), partial (Active) |
Mammalian cell |
CD47 Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-CF872379PI | Recombinant Pig Leukocyte surface antigen CD47 (CD47) |
in vitro E.coli expression system |
CSB-YP872379PI1 CSB-EP872379PI1 CSB-BP872379PI1 CSB-MP872379PI1 CSB-EP872379PI1-B |
Recombinant Pig Leukocyte surface antigen CD47 (CD47), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD47 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-CF885628BO | Recombinant Bovine Leukocyte surface antigen CD47 (CD47) |
in vitro E.coli expression system |
CSB-YP885628BO1 CSB-EP885628BO1 CSB-BP885628BO1 CSB-MP885628BO1 CSB-EP885628BO1-B |
Recombinant Bovine Leukocyte surface antigen CD47 (CD47), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD47 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-CF004940RA | Recombinant Rat Leukocyte surface antigen CD47 (Cd47) |
in vitro E.coli expression system |
CSB-YP004940RA1 CSB-EP004940RA1 CSB-BP004940RA1 CSB-MP004940RA1 CSB-EP004940RA1-B |
Recombinant Rat Leukocyte surface antigen CD47 (Cd47), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD47 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Code | Product Name | Source |
---|---|---|
CSB-CF729089PYX | Recombinant Pongo abelii Leukocyte surface antigen CD47 (CD47) |
in vitro E.coli expression system |
CSB-YP729089PYX1 CSB-EP729089PYX1 CSB-BP729089PYX1 CSB-MP729089PYX1 CSB-EP729089PYX1-B |
Recombinant Pongo abelii Leukocyte surface antigen CD47 (CD47), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD47 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-AP005201HU | Recombinant Human Leukocyte surface antigen CD47 (CD47), partial (Active) |
Mammalian cell |
CSB-AP005211HU | Recombinant Human Leukocyte surface antigen CD47 (CD47), partial (Active) |
Mammalian cell |
CSB-MP004940HU | Recombinant Human Leukocyte surface antigen CD47 (CD47), partial (Active) |
Mammalian cell |
CSB-MP004940HUj8-B | Recombinant Human Leukocyte surface antigen CD47 (CD47), partial,Biotinylated |
Mammalian cell |
CD47 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL004940HU | Human Leukocyte surface antigen CD47(CD47) ELISA kit |
serum, plasma, urine, tissue homogenates | 0.78 pg/mL |
CD47 ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL004940MO | Mouse Leukocyte surface antigen CD47(CD47) ELISA kit |
serum, plasma,tissue homogenates | 3.9 pg/mL |