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Cathepsin F is a protein in humans that is encoded by CTSF gene. Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
The following CTSF reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CTSF Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA15279B0Rb | CTSF Antibody, HRP conjugated |
Human | ELISA |
| CSB-PA006189GA01HU | CTSF Antibody |
Human,Mouse,Rat | ELISA,IHC |
| CSB-PA15279A0Rb | CTSF Antibody |
Human | ELISA |
| CSB-PA15279C0Rb | CTSF Antibody, FITC conjugated |
Human |
CTSF Proteins for Mus musculus (Mouse)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP006189MO CSB-EP006189MO CSB-BP006189MO CSB-MP006189MO CSB-EP006189MO-B |
Recombinant Mouse Cathepsin F (Ctsf) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CTSF Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP006189HU CSB-EP006189HU CSB-BP006189HU CSB-MP006189HU CSB-EP006189HU-B |
Recombinant Human Cathepsin F (CTSF) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-RP152794h | Recombinant Human Cathepsin F protein (CTSF), partial |
E.coli |
Cathepsin F (CTSF) is a cysteine endopeptidase broadly expressed in various tissues and cells [1]. The CTSF gene was mapped to the long arm of chromosome 11 at position q13.1-3, a locus shared with CTSW. Sequence comparisons of the propeptides indicated that cathepsin F and cathepsin W may form a new cathepsin subgroup. Like other cathepsins, CTSF is synthesized as an inactive pro-cathepsin F, and it is processed to become mature and active enzymes. Mature cathepsin F was highly active with comparable specific activities toward synthetic substrates as reported for cathepsin L [2]. CTSF has a broad pH optimum between 5.2 and 6.8 [2]. Its pH stability at cytosolic pH (7.2) was short, with a half-life of approximately 2 minutes [2]. As a subset of antigen-presenting cells (APCs), CTSF can efficiently degrade the MHC class II-associated invariant (Ii) chain [3]. The CTSF mRNA level was associated with tumor differentiation, depth of tumor invasion, and lymph node metastasis. Ji C et al. demonstrated that downregulation of CTSF expression efficiently inhibited apoptosis and promoted the proliferation of gastric cancer (GC) cells and suggested CTSF may be a potential therapeutic target in the treatment of GC, one of the most common cancers in the world [4]. Overexpression of CTSF is involved in the pathogenesis of cervical carcinoma (CC), one of the most common cancers among women worldwide [5].
[1] Santamaria I., Velasco G., et al. Molecular cloning and structural and functional characterization of human cathepsin F, a new cysteine proteinase of the papain family with a long propeptide domain [J]. J. Biol. Chem 1999, 274, 13800-13809.
[2] B Wang, G P Shi, Human Cathepsin F. Molecular Cloning, Functional Expression, Tissue Localization, and Enzymatic Characterization [J]. J Biol Chem, 1998, 273 (48), 32000-8.
[3] Shi GP, Bryant RA, et al. Role for cathepsin F in invariant chain processing and major histocompatibility complex class II peptide loading by macrophages [J]. J Exp Med. 2000 Apr 3; 191(7):1177-86.
[4] Ji C, Zhao Y, et al. Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells [J]. Oncol Res. 2018 Jan 19;26(1):83-93.
[5] Vazquez-Ortiz G, Pina-Sanchez P, et al. Overexpression of cathepsin F, matrix metalloproteinases 11 and 12 in cervical cancer [J]. BMC Cancer. 2005 Jun 30;5:68.
