Code | CSB-RA007479A1092phHU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
The production of the phospho-EGFR (Y1092) recombinant monoclonal antibody involves the application of protein technology and DNA recombinant techniques. The process begins by immunizing animals with a synthesized peptide derived from human phospho-EGFR (Y1092), followed by the isolation of B cells. Positive B cells are then selected and undergo single clone identification. The light and heavy chains of the phospho-EGFR (Y1092) antibody are amplified through PCR and inserted into a plasmid vector to create a recombinant vector. This recombinant vector is transfected into host cells for antibody expression. The phospho-EGFR (Y1092) recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. It serves as a valuable tool for the detection of human phospho-EGFR (Y1092) protein in ELISA and WB applications.
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