ERG

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Overlay Peak curve showing jurkat cells stained with CSB-RA274766A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunoprecipitating ERG in Rat heart tissue
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA007781HA01HU in Rat heart tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA007781HA01HU (8µg) + Rat heart tissue (500µg)
Lane 3: Rat heart tissue (10µg)

Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA007781HA01HU at dilution of 1:100

Immunofluorescent analysis of PC-3 cells using CSB-PA007781HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)