Rat Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

Code CSB-E04757r
Size 96T,5×96T,10×96T
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Product Details

Target Name vascular endothelial growth factor A
Alternative Names Vegfa ELISA Kit; Vegf ELISA Kit; Vascular endothelial growth factor A ELISA Kit; VEGF-A ELISA Kit; Vascular permeability factor ELISA Kit; VPF ELISA Kit
Abbreviation VEGFA
Uniprot No. P16612
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.9 pg/mL-250 pg/mL
Sensitivity 0.97 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 84-101  
1:2 Average % 94  
Range % 90-100  
1:4 Average % 96  
Range % 91-103  
1:8 Average % 94  
Range % 86-100  
The recovery of rat VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-97  
EDTA plasma (n=4) 97 92-102  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
250 2.712 2.892 2.802 2.614  
125 2.369 2.317 2.343 2.155  
62.5 1.568 1.604 1.586 1.398  
31.25 0.877 0.888 0.883 0.695  
15.6 0.558 0.562 0.560 0.372  
7.8 0.371 0.384 0.378 0.190  
3.9 0.276 0.286 0.281 0.093  
0 0.188 0.187 0.188    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


Target Data

Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).
Gene References into Functions
  1. Our main findings demonstrate that 2-h swim exercise rapidly (i.e., immediately postexercise) increased Vegf-a mRNA levels in both subcutaneous (iWAT) and intra-abdominal (eWAT, rpWAT) adipose tissue of male Wistar rats after 2 weeks of high fat diet but not in rats kept on low fat diet. PMID: 29486133
  2. the epigenetic mechanisms involved in the physical exercise-mediated induction of VegfA expression, were examined. PMID: 27975171
  3. Our data suggest that activation of the GPR91-ERK1/2-C/EBP beta (c-Fos, HIF-1a) signaling pathway plays a tonic role in regulating VEGF transcription in rat retinal ganglion cells. PMID: 28374767
  4. present results suggested that pathologically high cyclic stretch induces the shuttling of SRSF1 which results in the secretive pattern splicing of VEGFA and finally contributes to the proliferation of vascular smooth muscle cells. PMID: 29974845
  5. Results indicate that vascular endothelial growth factor (VEGF) is a vital important factor that contributes to the small-airway remodelling in a rat model of Chronic obstructive pulmonary disease through promoting angiogenesis, which mainly depend on the specific binding between VEGF and VEGF receptor 1 and can be effectively attenuated by sunitinib. PMID: 28117425
  6. 150-kDa oxygen-regulated protein (ORP150) levels were significantly increased in rat retinas after induction of diabetes. Vascular endothelial growth factor (VEGF) and the pro-inflammatory cytokines interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha) induced upregulation of ORP150 in HRMEC. PMID: 29098793
  7. Expression of vascular endothelial growth factor in cardiac repair: Signaling mechanisms mediating vascular protective effects PMID: 29462681
  8. VEGF-A expression in soft tissues repaired by shockwave therapy PMID: 29921384
  9. While the mRNA expression of the proangiogenic factors VEGF, transforming growth factor-beta1 (TGF-beta1), and adrenomedullin dropped at 0 h post-hypoxia compared to normoxic control, no changes were detected at the protein level, showing an impaired response of these proangiogenic factors to hypoxia in the aged striatum PMID: 28501895
  10. Kisspeptin-10 prevents the increased vascular permeability of ovarian hyperstimulation syndrome by the activation of KISS1R and the inhibition of VEGF. PMID: 28676533
  11. The results indicate that VEGF promotes endothelial progenitor cell differentiation and vascular repair through Cx43. PMID: 29065929
  12. The results obtained in the study revealed that down-regulation of VEGF by RNAi could be a novel target approach for the treatment of failure (UFF) in rats with peritoneal dialysis. PMID: 28733472
  13. VEGF165 induces differentiation of hair follicle stem cells into endothelial cells and plays a role in in vivo angiogenesis. PMID: 28244687
  14. Enhanced neuroprotective efficacy of bone marrow mesenchymal stem cells co-overexpressing BDNF and VEGF in a rat model of cardiac arrest-induced global cerebral ischemia PMID: 28492549
  15. this is the first report describing the use of novel polymeric gene carriers for the delivery of VEGF gene to DA neurons with improved transfection efficiency. Finally, the study will lead to a significant advancement in the field of non-viral Parkinson's Disease gene therapy treatment. PMID: 28025049
  16. the results indicate that, Osx-overexpression promotes ADSCs' osteogenesis both in vitro and in vivo, which could be enhanced by release of VEGF. PMID: 28107808
  17. Following IL-1beta (50mug/kg ip.) administration, a higher serum IL-1beta level was detected in females than in males. In male rats, IL-1beta decreased BDNF mRNA in all the brain structures, except for the pituitary, and VEGF mRNA in the amygdala. PMID: 27744091
  18. VEGF knockdown can enhance chondrogenesis and prevent osteoarthritis progression. PMID: 27164955
  19. Here we report for the first time the expression of a rare and unusually potent splice variant, VEGF 111 , in vivo in mammals. Our discovery of VEGF 111 in the uterus of both a eutherian (rat) and a marsupial (fat-tailed dunnart) suggests that the splice variant may be common to all mammals PMID: 27722773
  20. Data indicate vascular endothelial growth factor A (VEGFA) as a target gene of miR-200b. PMID: 28122882
  21. Our results suggest that hUCMSCs can promote ovarian expression of HGF, VEGF, and IGF-1 through secreting those cytokines, resulting in improving ovarian reserve function and withstanding ovarian senescence. PMID: 28279229
  22. We demonstrate that a fine balance of VEGF and angiopoietin signaling is required for the formation of a functional vascular network. PMID: 27868196
  23. results indicate that the paracrine factor, VEGF, derived from transplanted BM-MSCs, regulated the expression of miRNAs such as miRNA-23a and miRNA-92a and exerted anti-apoptotic effects in cardiomyocytes after MI PMID: 28662151
  24. VEGF expression increased markedly in the cerebellum and mildly in the occipital lobe following up to 4 weeks of exposure to 20-ppm methylmercury. VEGF expression was detected mainly in astrocytes of the blood brain barrier. Intravenous administration of anti-VEGF neutralizing antibody mildly reduced the rate of hind-limb crossing signs observed in MeHg-exposed rats. PMID: 28118383
  25. Overexpressed long non-coding RNA (lncRNA) ANRIL upregulates vascular endothelial growth factor (VEGF) and promotes angiogenesis by activating NF-kappa B (NF-kappaB) signaling pathway in diabetes mellitus (DM) combined with cerebral infarction (DM + CI) rats. PMID: 28060742
  26. Vitexicarpine increased rats' survival time and decreased serum alpha-fetoprotein as well as it ameliorated fibrosis and massive hepatic tissue breakdown. It attenuated hepatocellular carcinoma-induced protein and gene expression of vascular endothelial growth factor PMID: 28651490
  27. Knockdown of hepatic VEGF decreases hepatic sdf1 expression and plasma sdf1 levels. PMID: 26939868
  28. XBP1 regulates VEGF-mediated cardiac angiogenesis. PMID: 27133203
  29. a novel rodent model to reproduce much of the known phenotype of CTEPH, including the pivotal pathophysiological role of impaired vascular endothelial growth factor-dependent vascular remodeling, is reported. PMID: 28183533
  30. VEGF treatment increases expression of HGF in vitro and in vivo and that, based on a series of integrative studies, we suggest that the effects of VEGF are mediated through downstream HGF signaling. PMID: 27036872
  31. MiR-21 exerts protective effects on endothelial injury through the PTEN/VEGF pathway after acute myocardial infarction. PMID: 27708252
  32. Chemerin and VEGF play important roles in diabetic nephropathy. PMID: 27612613
  33. Vegf expression is increased in brain from ischemic rats following treadmill exercise. PMID: 27747480
  34. these results suggested that miRNA-29a suppresses cardiac fibrosis and fibroblasts proliferation via targeting VEGF-A/MAPK signal pathway implicating that miRNA-29a might play a role in the treatment of cardiac fibrosis. PMID: 27060017
  35. Data suggest that a vascular endothelial growth factor (VEGF) signaling defect possibly plays an important role in defective embryonic airway branching. PMID: 27372649
  36. the inhibitory effect of miR1405p on angiogenesis was attenuated by the overexpression of VEGFA. PMID: 27035554
  37. we aimed to investigate the effect on angiogenesis of endoglin and VEGF expression in 1, 2-dimethylhydrazine dihydrochloride (DMH)-induced rat colon carcinogenesis and evaluated the implementation of COX2 inhibitors for molecular targets on tumor considering the above-mentioned mechanisms. PMID: 27466499
  38. This study demonstrated that vegf increases in male rat with aneurysmal subarachnoid hemorrhage. PMID: 26923576
  39. This study demonstrates that hyperthyroidism may have an effect on the development of rat adrenal glands mediated by VEGF expression, angiogenesis, and apoptosis. PMID: 26708182
  40. This PHD-2-mediated transcriptional activation may be important for regulating VEGF expression through HIF-1a signaling in LCs during corpus luteum development in mammals. PMID: 26681021
  41. Results indicate that chronic stress enhances vulnerability of brain-derived neurotrophic factor and vascular endothelial growth factor response to neuroinflammation. PMID: 26396035
  42. Identify a mechanism of VEGF overexpression in liver and mesentery that promotes pathologic, but not physiologic, angiogenesis, via sequential and nonredundant functions of CPEB1 and CPEB4. PMID: 26627607
  43. The increased level of VEGF expression in the nicotine-treated group may have affected endothelial cell apoptosis PMID: 27363062
  44. TP53, BCL-2, and VEGFA genes may participate in the proliferation of esophagus cancer cells in a synergistic manner PMID: 26439224
  45. results indicate OVX may induce 3-D capillary regression in soleus muscle through an imbalance between VEGF-A and TSP-1 expression, possibly associated with decreased exercise tolerance in ovariectomized rats. PMID: 26092525
  46. PHD2-mediated transcriptional activation may be one of the mechanisms regulating VEGF expression in luteal cells during mammalian corpus luteum development. PMID: 25975603
  47. Cardiomyocyte VEGF activates EC Notch signaling, facilitating GPIHBP1-mediated translocation of LPL across EC and regulating LPL-derived fatty acid delivery to the cardiomyocytes. PMID: 26586663
  48. Expression in control and blank groups was similar at 3 and 6 months. Atorvastatin can inhibit VEGI and vascular endothelial growth factor expression to protect rats from diabetic retinopathy PMID: 26125742
  49. VEGF is implicated in the pathogenesis of inflammation after electrical burns. PMID: 25466960
  50. No significant differences were noted in the expression of VEGF in rat tympanic membrane perforation. PMID: 25920966
  51. hyperglycemia causes succinate accumulation and GPR91 activity in retinal ganglion cells, which mediate VEGF-induced retinal vascular change via the ERK1/2/COX-2/PGE2 pathway PMID: 25324681
  52. The data implied that miR-210 played an important role in ameliorating the estrogen deficiency caused-postmenopausal osteoporosis through promotion the VEGF expression and osteoblast differentiation. PMID: 25503465
  53. This study demonstrated that Chronic restraint stress increases the protein expression of VEGF in rats. PMID: 25655083
  54. VEGF mRNA and protein expression levels increased in day 10, while the expression of miR-126 was remarkably down-regulated PMID: 26413624
  55. Autoantibodies in dilated cardiomyopathy induce VEGF expression in cardiomyocytes. PMID: 26248134
  56. results suggested that VEGF165 attenuated the spinal cord injury by inhibiting the inflammation and increasing the autophagy function PMID: 26116774
  57. The increases in both BBB permeability and VEGF expression in the brain cortex of RIBI groups at same time period confirmed the possibility of brain injury following irradiation of 6 Gy. PMID: 25279427
  58. Chronic nerve compression activates transcription factors such as NF-kappaB and p53, which are key effectors of the cellular stress response, suggesting a neuroprotective process associated with an increased VEGF-A-mediated neurotrophic effect. PMID: 24865514
  59. Increased expression of Ang-1, Ang-2, Tie-2, and VEGF in ulcerative colitis-derived colorectal cancer might lead to chronic colitis and pathologic angiogenesis in rats. PMID: 25759532
  60. Although no difference was found in anxiety levels as a result of involuntary exercise in males, females showed increased anxiety levels, and decreased VEGF and BDNF levels in the prefrontal cortex after involuntary exercise PMID: 25203492
  61. Finasteride reduces the VEGF expression and decreases the MVD in the renal tissue of diabetic rats, suggesting the therapeutic potential of finasteride on diabetic microvascular complications. PMID: 25789687
  62. Intravitreal treatment with VEGF increases PEDF levels and reduces the apoptosis of RGCs in rats with chronic intraocular hypertension. PMID: 25337213
  63. the obviously decreased serum PEDF concentration and the abnormal enhanced expression of VEGF density in local retinal tissue broke down the balance of PEDF/VEGF in whole-body or local tissues, which might play an important role in retinal vascular proliferation. PMID: 25673207
  64. Data indicate that MiR-377 acts directly at the 3'UTR of vascular endothelial growth factor (VEGF). PMID: 25251394
  65. The expression of VEGF was found to be beneficial for functional recovery following cerebral ischemic injury in rats. PMID: 25174606
  66. Dynamic changes in VEGF expression in the rat cerebral cortex following gamma knife surgeries. PMID: 25176344
  67. This study demonistrated that the relative levels of alternatively spliced VEGF-A isoforms are critical for pain modulation under both normal conditions and in sensory neuropathy. PMID: 25151644
  68. This work suggested that VEGF signaling defect plays an important role in the defective lung development following maternal protein malnutrition PMID: 25031729
  69. Fractionated gamma-irradiation with fixed total dose differently modified VEGF, GFAP, and BDNF gene expression in the brain tissue. PMID: 25110093
  70. VEGF expression in retinal Muller cells induced by high-glucose is dependent on intracellular calcium. PMID: 22796763
  71. Goto-Kakizaki diabetic rats manifested a premature increase in chondrocyte apoptosis in the growth plate, and a compensatory overexpression of chondroregulatory proteins, such as PTHrP, Runx2, and VEGF. PMID: 25159845
  72. High-glucose PD fluid and uraemic circumstance resulted in the abnormal expression of TTP and the VEGF family in a PD time-dependent manner; this may lead to UFF through angiogenesis and lymphangiogenesis PMID: 24696420
  73. Catwalktrade mark analysis showed AdV-ZFP-VEGF treatment dramatically improves hindlimb weight support (p<0.05) and increases hindlimb swing speed (p<0.02) when compared to control animals PMID: 24846143
  74. Given the dose-dependent inhibitory effect of D2-ags on ovarian VEGF production reported herein, we infer that current OHSS therapies used in humans may be improved by increasing the intraovarian concentration of D2-ags in these patients. PMID: 25217874
  75. A decrease of VEGF and NGF-beta levels is associated with the increase of the apoptosis and testicular damage in diabetic rats. PMID: 24771956
  76. these findings indicate that carbon ion may be superior to X-ray radiation for inhibiting tumorigenesis and angiogenesis through modulation of VEGF level in the glioma microenvironment. PMID: 24893038
  77. Studied exercise training effects on age-related changes in cerebral blood flow and capillary vascularity through upregulation of VEGF and eNOS. PMID: 24822184
  78. The results of this study show that inhibiting VEGF may have detrimental effects on the apoptosis of neuronal cells in the inner layers of the diabetic retina. PMID: 24709590
  79. Increased vascular resistance, secondary to reduced VEGF-incduced nitric oxide release, contributes to blood pressure increases. PMID: 24329544
  80. These results suggest that activation of the VEGF/VEGFR signaling pathway is important in the formation of venous hypertension-induced DAVFs. PMID: 24626343
  81. VEGF signaling at vascular blood-neural barriers is different depending on whether the agonist is tissue or blood borne PMID: 25175707
  82. VEGF secreted by transplanted MSCs is one of the critical paracrine factors that play seminal roles in attenuating hyperoxic lung injuries in neonatal rats PMID: 24669883
  83. We found that vasohibin-1 and VEGF are up-regulated, in mesentery and liver, in cirrhotic and precirrhotic portal hypertensive rats and cirrhosis patients. PMID: 24390792
  84. The synergistic effects of VEGF and Ang-2 may be responsible for the angiogenesis following cerebral infarction. PMID: 23073804
  85. VEGF-A promoted the production of astrocytes from SVZ glial progenitors while VEGF-C stimulated the proliferation of both early and late oligodendrocyte progenitors during recovery from neonatal hypoxia-ischemia PMID: 23565129
  86. R3V6 delivered both VEGF-siRNA and BCNU efficiently into the glioblastoma cells. PMID: 24219243
  87. Administration of Ad-HIF-1alpha after spinal cord injury could ameliorate neuronal apoptosis and promote angiogenesis in rats via Bax/VEGF pathway. PMID: 24228092
  88. The palmitate may increase the expression and secretion of Vegf120 through, mainly, the PI3K pathways in UMR-106 cells. PMID: 24491567
  89. COX-2 could increase vascular endothelial growth factor (VEGF)-A and VEGF-C expressions in peritoneal tissue, resulting in increased formation of peritoneal and lymphatic microvessels playing pivotal roles in the development of ultrafiltration failure. PMID: 24559584
  90. VEGF induced vascularisation in the bony repair of injured growth plate cartilage. PMID: 24464023
  91. data suggested that the extracellular matrix stiffness stimulation signal was transduced into HCC cells via integrin beta1, and this signal activated the PI3K/Akt pathway and upregulated VEGF expression PMID: 24472554
  92. Data indicate that FTY720 treatment changes the expression of vascular endothelial growth factor alpha (VEGF alpha) in the periinfarct cortex. PMID: 23936150
  93. Importin-alpha is a novel and critical regulator of gastric angiogenesis via alterations in VEGF expression. PMID: 24356884
  94. Endogenous expression of human and rat VEGF-A165b was detected in hippocampal and cortical neurons. PMID: 23838428
  95. Knockdown of up-regulated VEGFA in cells that overexpress it under pathological conditions would reduce intravitreous neovascularization without affecting physiological retinal vascular development. PMID: 23972394
  96. Social isolation impairs oral palatal wound healing in rats; VEGFA is a direct target for miR-29- and miR-203-mediated suppression. PMID: 23951316
  97. Studied the expression and significance of vascular endothelial growth factor (VEGF) and p53 expression in rat degenerated intervertebral disc (IVD) tissues. PMID: 23608382
  98. Osteopontin induces the release of VEGF from retinal Muller cells by inhibiting osmotic swelling. PMID: 23639879
  99. Significant changes in the proportion of Vascular endothelial growth factor a were noted mainly in the matrix-bound variants Vegf144 and Vegf188 in microvascular endothelial cells and osteoblasts, respectively. PMID: 23483612
  100. Hypoxic induction did not alter this ranking, although it did favor an increased stoichiometry of Vegf164 mRNA over the other two splice variants. PMID: 24076411

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Subcellular Location Secreted
Protein Families PDGF/VEGF growth factor family
Tissue Specificity Expressed in the pituitary, in brain, in particularly in supraoptic and paraventricular nuclei and the choroid plexus. Also found abundantly in the corpus luteum of the ovary and in kidney glomeruli. Expressed in the ductal epithelial cells of post-pubert
Database Links

KEGG: rno:83785

STRING: 10116.ENSRNOP00000026637

UniGene: Rn.1923

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