Rat Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

Rat Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

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Rat Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

Specification
Target

Code

CSB-E04757r

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P16612

Lead Time

3-5 working days

Abbreviation

VEGFA

Protein Biological Process 1

Angiogenesis

Target Name

vascular endothelial growth factor A

Alias

RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor

Species

Rattus norvegicus (Rat)

Protein Biological Process 3

Angiogenesis

Sample Types

serum, plasma, tissue homogenates

Detection Range

3.9 pg/mL-250 pg/mL

Sensitivity

0.97 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	84-101
1:2	Average %	94
	Range %	90-100
1:4	Average %	96
	Range %	91-103
1:8	Average %	94
	Range %	86-100

Recovery

The recovery of rat VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	97	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
250	2.712	2.892	2.802	2.614
125	2.369	2.317	2.343	2.155
62.5	1.568	1.604	1.586	1.398
31.25	0.877	0.888	0.883	0.695
15.6	0.558	0.562	0.560	0.372
7.8	0.371	0.384	0.378	0.190
3.9	0.276	0.286	0.281	0.093
0	0.188	0.187	0.188

References

Vasoprotective effect of vitamin E: Rescue of ethanol-induced atherosclerosis and inflammatory stress in rat vascular wall Alireza Shirpoora et al,International Immunopharmacology,2013 May 9.pii: S1567-5769(13)00170-7.
Angiotensin II type 2 receptor correlates with therapeutic effects of losartan in rats with adjuvant-induced arthritis Di Wang et al,Journal of Cellular and Molecular Medicine ,2013 Dec;17(12):1577-87
Aloe vera and Vitis vinifera improve wound healing in an in vivo rat burn wound model Lin.et al,Molecular Medicine Reports,2015 1070-1076
THE EFFECT OF MIDKINE ON GROWTH FACTORS AND OXIDATIVE STATUS IN AN EXPERIMENTAL WOUND MODEL IN DIABETIC AND HEALTHY RATS BURAK DiK.et al,Canadian Journal of Physiology and Pharmacology,Dec 23
Enhanced hypoxic tolerance by Seabuckthorn is due to upregulation of HIF-1伪 and attenuation of ER stress Kanika Jain.et al,Applied Biomedicine,2016
Synergistic Effects of Aerobic Exercise after BoneMarrow Stem Cell Transplantation on Recovery of Dopaminergic Neurons and AngiogenesisMarkers of Parkinsonian Rats Seyed Abdollah Hashemvarzi.et al,IJAEP,2016 5(1)
Ferulic acid alleviates symptoms of preeclampsia in rats by upregulating vascular endothelial growth factor Gong W.et al,Clin Exp Pharmacol Physiol. ,Jun 22
Protective role of B-patchoulene from Pogostemon cablin against indomethacin-induced gastric ulcer in rats: Involvement of anti-inflammation Jia-Zhen Wu.et al,Phytomedicine,Dec 25
Effect of Niacin on Inflammation and Angiogenesis in a Murine Model of Ulcerative Colitis Salem HA.et al,Sci Rep,Aug 2
The role of resveratrol on full - Thickness uterine wound healing in rats. Sayin O.et al,Taiwan J Obstet Gynecol.,Oct
Preconditioning Effect of Aerobic Exercise with Vitamin D3 Intake on VEGF Levels in 6-OHDA-Lesioned Rat Model of Parkinson's Disease Seyed Abdollah Hashemvarzi.et al,Journal of Basic and Clinical ,Sep 3
Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo Tian Zhou.et al,Int J Nanomedicine,May 2
Metformin prevents hormonal and metabolic disturbances and 1, 2-dimethylhydrazine-induced colon carcinogenesis in non-diabetic rats Viktoria V. Bekusova.et al,Cancer Biol Med,/
Oligo-peptide ICF-6 inhibits hepatic stellate cell activation and ameliorates CCl4-induced liver fibrosis by suppressing NF-kB signaling and Wnt/B-catenin Haitao Sun.et al,Journal of Pharmacological Sciences,Feb 2
Tunable sequential drug delivery system based on chitosan/hyaluronic acid hydrogels and PLGA microspheres for management of non-healing infected wounds JinjianHuang.et al,Materials Science and Engineering: C,11 April
Diabetex: A novel approach for diabetic wound healing Alshaymaa Amin ZakiEl-Bahy.et al,Life Sciences,June 25
Olopatadine enhances recovery of alkali-induced corneal injury in rats SamahKandeel.et al,Life Sciences,July 3
Bevacizumab-treated pregnant rats may constitute an experimental model for studying preeclampsia Abdullah Boztosun.et al,Pamukkale Medical Journal,/
Subcutaneous Inoculation of Echinococcus multilocularis Induces Delayed Regeneration after Partial Hepatectomy Apaer S, et al,Scientific Reports,January 24

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Function	Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).
Subcellular Location	Secreted
Protein Families	PDGF/VEGF growth factor family
Tissue Specificity	Expressed in the pituitary, in brain, in particularly in supraoptic and paraventricular nuclei and the choroid plexus. Also found abundantly in the corpus luteum of the ovary and in kidney glomeruli. Expressed in the ductal epithelial cells of post-pubert
Database Links	KEGG: rno:83785 STRING: 10116.ENSRNOP00000026637 UniGene: Rn.1923
Pathway	HIF-1 signaling pathway MAPK signaling pathway PI3K-Akt signaling pathway VEGF signaling pathway Focal adhesion Ras signaling pathway AGE-RAGE signaling pathway in diabetic complications Rap1 signaling pathway

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.