Rat Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

Code CSB-E04757r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P16612
Lead Time 3-5 working days
Abbreviation VEGFA
Protein Biological Process 1 Angiogenesis
Target Name vascular endothelial growth factor A
Alias RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Angiogenesis
Sample Types serum, plasma, tissue homogenates
Detection Range 3.9 pg/mL-250 pg/mL
Sensitivity 0.97 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 84-101  
1:2 Average % 94  
Range % 90-100  
1:4 Average % 96  
Range % 91-103  
1:8 Average % 94  
Range % 86-100  
Recovery
The recovery of rat VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-97  
EDTA plasma (n=4) 97 92-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
250 2.712 2.892 2.802 2.614  
125 2.369 2.317 2.343 2.155  
62.5 1.568 1.604 1.586 1.398  
31.25 0.877 0.888 0.883 0.695  
15.6 0.558 0.562 0.560 0.372  
7.8 0.371 0.384 0.378 0.190  
3.9 0.276 0.286 0.281 0.093  
0 0.188 0.187 0.188    
References
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Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).
Subcellular Location Secreted
Protein Families PDGF/VEGF growth factor family
Tissue Specificity Expressed in the pituitary, in brain, in particularly in supraoptic and paraventricular nuclei and the choroid plexus. Also found abundantly in the corpus luteum of the ovary and in kidney glomeruli. Expressed in the ductal epithelial cells of post-pubert
Database Links

KEGG: rno:83785

STRING: 10116.ENSRNOP00000026637

UniGene: Rn.1923

Pathway HIF-1 signaling pathway
MAPK signaling pathway
PI3K-Akt signaling pathway
VEGF signaling pathway
Focal adhesion
Ras signaling pathway
AGE-RAGE signaling pathway in diabetic complications
Rap1 signaling pathway

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