Recombinant Arabidopsis thaliana Potassium channel AKT1(AKT1) ,partial

Code CSB-YP655043DOA
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Source Yeast
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Code CSB-EP655043DOA
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Source E.coli
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Code CSB-EP655043DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP655043DOA
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Source Baculovirus
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Code CSB-MP655043DOA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names AKT1
Uniprot No. Q38998
Alternative Names AKT1; At2g26650; F18A8.2Potassium channel AKT1
Species Arabidopsis thaliana (Mouse-ear cress)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Highly selective inward-rectifying potassium channel that mediate potassium uptake by plant roots in response to low K(+) conditions, by a calcium-, CBL-, and CIPK-dependent pathway. Positively regulated by phosphorylation by CIPK23. Negatively regulated by a kinase-independent regulatory mechanism involving a competing direct binding of CBL10. Involved in the stomatal regulation by monitoring the turgor pressure in guard cells. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by hyperpolarization. May interact with the cytoskeleton or with regulatory proteins. Is essential with POT5/HAK5 for high-affinity potassium uptake in roots during seedling establishment and postgermination growth under low potassium conditions.
Gene References into Functions
  1. CIPK23 and AtKC1 exhibit distinct effects; however, they act synergistically and balance K(+) uptake/leakage to modulate AKT1-mediated low potassium responses in Arabidopsis. PMID: 26829980
  2. results suggest that NO decreases K(+) absorption by promoting the synthesis of vitamin B6 PLP, which further represses the activity of K(+) channel AKT1 in Arabidopsis. PMID: 25355908
  3. Examination of the athak5 atakt1 double mutant, revealing novel aspects of an uptake system as yet unidentified by genetic means. PMID: 23553635
  4. AKT1 is regulated by CIPK23 in guard cells and is involved in water stress responses. PMID: 22210899
  5. These findings provide further insights into the signaling network consisting of CBL-CIPK-PP2C interactions in the activation of the AKT1 channel. PMID: 21596690
  6. Electrophysiological results showed that AtKC1 inhibited the AKT1-mediated inward K(+) currents and negatively shifted the voltage dependence of AKT1 channels. PMID: 20514083
  7. AtHAK5 and AKT1 are vital for plant growth and development at low K+ concentrations. PMID: 20413648
  8. In the range between 0.01 and 0.05 mM K+ AtHAK5 and AtAKT1 are the only contributors to K+ acquisition. At higher K+ concentrations, unknown systems come into operation and participate together with AtAKT1 in low-affinity K+ uptake. PMID: 20088908
  9. Potassium transport and gene expression in A. thaliana deficient at this site. PMID: 15734909
  10. CIPK23 directly phosphorylates the K+ transporter AKT1 PMID: 16814720
  11. Data show that interacting calcium sensors (CBL1 and CBL9) together with CIPK23, but not either alone, activated the AKT1 channel in a Ca(2+)-dependent manner, connecting the Ca(2+) signal to K(+) uptake through activation of a K(+) channel. PMID: 16895985
  12. AtAKT1 mediates K+ absorption at high concentrations, and remains active in the presence of NH4+ although activity is reduced. PMID: 19000196
  13. AtKC1-AKT1 channels in Arabidopsis roots facilitate growth under K+-limiting conditions PMID: 19509299

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Subcellular Location Cell membrane; Multi-pass membrane protein.
Protein Families Potassium channel family, Plant (TC 1.A.1.4) subfamily
Tissue Specificity Preferentially expressed in the peripheral cell layers of root mature including root cortex and root hairs. Detected also, at a lower level, in the mesophyll of the leaves and at restricted sites corresponding to hydathodes and guard cells.
Database Links

KEGG: ath:AT2G26650

STRING: 3702.AT2G26650.1

UniGene: At.48521

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