AKT1 Antibody

Code CSB-PA655043XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AKT1 Polyclonal antibody
Uniprot No.
Target Names
AKT1
Alternative Names
AKT1 antibody; At2g26650 antibody; F18A8.2Potassium channel AKT1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AKT1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Highly selective inward-rectifying potassium channel that mediate potassium uptake by plant roots in response to low K(+) conditions, by a calcium-, CBL-, and CIPK-dependent pathway. Positively regulated by phosphorylation by CIPK23. Negatively regulated by a kinase-independent regulatory mechanism involving a competing direct binding of CBL10. Involved in the stomatal regulation by monitoring the turgor pressure in guard cells. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by hyperpolarization. May interact with the cytoskeleton or with regulatory proteins. Is essential with POT5/HAK5 for high-affinity potassium uptake in roots during seedling establishment and postgermination growth under low potassium conditions.
Gene References into Functions
  1. CIPK23 and AtKC1 exhibit distinct effects; however, they act synergistically and balance K(+) uptake/leakage to modulate AKT1-mediated low potassium responses in Arabidopsis. PMID: 26829980
  2. results suggest that NO decreases K(+) absorption by promoting the synthesis of vitamin B6 PLP, which further represses the activity of K(+) channel AKT1 in Arabidopsis. PMID: 25355908
  3. Examination of the athak5 atakt1 double mutant, revealing novel aspects of an uptake system as yet unidentified by genetic means. PMID: 23553635
  4. AKT1 is regulated by CIPK23 in guard cells and is involved in water stress responses. PMID: 22210899
  5. These findings provide further insights into the signaling network consisting of CBL-CIPK-PP2C interactions in the activation of the AKT1 channel. PMID: 21596690
  6. Electrophysiological results showed that AtKC1 inhibited the AKT1-mediated inward K(+) currents and negatively shifted the voltage dependence of AKT1 channels. PMID: 20514083
  7. AtHAK5 and AKT1 are vital for plant growth and development at low K+ concentrations. PMID: 20413648
  8. In the range between 0.01 and 0.05 mM K+ AtHAK5 and AtAKT1 are the only contributors to K+ acquisition. At higher K+ concentrations, unknown systems come into operation and participate together with AtAKT1 in low-affinity K+ uptake. PMID: 20088908
  9. Potassium transport and gene expression in A. thaliana deficient at this site. PMID: 15734909
  10. CIPK23 directly phosphorylates the K+ transporter AKT1 PMID: 16814720
  11. Data show that interacting calcium sensors (CBL1 and CBL9) together with CIPK23, but not either alone, activated the AKT1 channel in a Ca(2+)-dependent manner, connecting the Ca(2+) signal to K(+) uptake through activation of a K(+) channel. PMID: 16895985
  12. AtAKT1 mediates K+ absorption at high concentrations, and remains active in the presence of NH4+ although activity is reduced. PMID: 19000196
  13. AtKC1-AKT1 channels in Arabidopsis roots facilitate growth under K+-limiting conditions PMID: 19509299

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Potassium channel family, Plant (TC 1.A.1.4) subfamily
Tissue Specificity
Preferentially expressed in the peripheral cell layers of root mature including root cortex and root hairs. Detected also, at a lower level, in the mesophyll of the leaves and at restricted sites corresponding to hydathodes and guard cells.
Database Links

KEGG: ath:AT2G26650

STRING: 3702.AT2G26650.1

UniGene: At.48521

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