Recombinant Drosophila melanogaster RAC serine/threonine-protein kinase(Akt1) ,partial

Code CSB-YP818217DLU
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Source Yeast
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Code CSB-EP818217DLU
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Source E.coli
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Code CSB-EP818217DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP818217DLU
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Source Baculovirus
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Code CSB-MP818217DLU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Akt1
Uniprot No. Q8INB9
Alternative Names Akt1; CG4006RAC serine/threonine-protein kinase; DAkt; DRAC-PK; Dakt1; EC 2.7.11.1; Akt; Protein kinase B; PKB
Species Drosophila melanogaster (Fruit fly)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Serine/threonine kinase involved in various developmental processes. During early embryogenesis, acts as a survival protein. During mid-embryogenesis, phosphorylates and activates trh, a transcription factor required for tracheal cell fate determination. Also regulates tracheal cell migration. Later in development, acts downstream of PI3K and Pk61C/PDK1 in the insulin receptor transduction pathway which regulates cell growth and organ size, by phosphorylating and antagonizing FOXO transcription factor. Controls follicle cell size during oogenesis. May also stimulate cell growth by phosphorylating Gig/Tsc2 and inactivating the Tsc complex. Dephosphorylation of 'Ser-586' by Phlpp triggers apoptosis and suppression of tumor growth.
Gene References into Functions
  1. The metabolic defects of cycG mutant animals are abrogated by a concomitant loss of Wdb, CycG presumably influences Akt1 activity at the PP2A nexus; Well rounded (Wrd), another B' subunit of PP2A in Drosophila, binds CycG similar to Wdb, and that its loss ameliorates some, but not all, of the metabolic defects of cycG mutants. PMID: 26980713
  2. The developmental delay of these novel Akt1 hypomorphs results in a latent phenotype uncovered by generation of somatic clones PMID: 25889856
  3. these data show that Drosophila Trbl has a conserved role to bind Akt and block Akt-mediated insulin signaling, and implicate Trib proteins as novel sites of signaling pathway integration that link nutrient availability with cell growth and proliferation PMID: 25329475
  4. Akt1 governs two critical elements of synapse development, neurotransmitter receptor localization, and postsynaptic membrane elaboration PMID: 23592328
  5. Regeneration of Drosophila sensory neuron axons and dendrites is regulated by the Akt pathway involving Pten and microRNA bantam. PMID: 22759636
  6. Overexpression of Akt enhanced Sindbis virus replication. PMID: 22258238
  7. dAkt activation under wild-type conditions is defined by feedback inhibition that depends on TOR Complex 1 PMID: 20585550
  8. A mutation in the pleckstrin homology (PH) domain of Akt that reduces its affinity for PIP3 sufficed to rescue the lethality of flies devoid of PTEN activity. PMID: 11872800
  9. stimulates growth by phosphorylating the tuberous sclerosis complex 2 (Tsc2) tumour suppressor and inhibiting formation of a Tsc1-Tsc2 complex PMID: 12172554
  10. cell growth control by PTEN and it's ability to regulate known cell size regulators involved in protein translation (review) PMID: 12367630
  11. In Drosophila cells, Drosophila protein kinase B is required for the activation of Drosophila p70 ribosomal S6 protein kinase by insulin. PMID: 12841848
  12. InR regulates cell growth and proliferation through the dAkt pathway. PMID: 12893776
  13. Although S6K phosphorylation is independent of phosphoinositide 3-OH kinase (PI3K) and serine/threonine protein kinase Akt, that of 4E-BP is dependent on PI3K and Akt. PMID: 14645523
  14. results show show that target of rapamycin (TOR) kinase and its associated protein rictor are necessary for AKT/PKB Ser473 phosphorylation and that a reduction in rictor or drosophila TOR (dTOR) expression inhibited an Akt/PKB effector PMID: 15718470
  15. The characterization of synaptic long-term depression (LTD) at the Drosophila requires AKt protein. PMID: 16611817
  16. Ras-phosphatidylinositol 3-kinase (PI3K)-Akt activity in the peripheral glia promotes growth of the perineurial glia by inhibiting forkhead box O (FOXO). PMID: 17215387
  17. both AKT and HSP70 alleviated mHtt-induced toxicity in the retina. In contrast, their protective effects differed in the brain PMID: 18065778
  18. Akt regulates a complex containing Zw3, Armadillo, APC2, and EB1 and that this complex has a role in stabilizing MT-cortex interactions, facilitating both centrosome separation and mitotic spindle orientation. PMID: 18268102
  19. The protein phosphatase PP2A-B' subunit Widerborst is a negative regulator of cytoplasmic activated Akt and lipid metabolism in Drosophila PMID: 18827008
  20. shows that phosphorylation of the Tsc1/2 complex by Akt is not required for Akt to activate TORC1 and to promote tissue growth in Drosophila PMID: 19609361

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Subcellular Location Cytoplasm, cytosol. Cell membrane. Note=Recruited to plasma membrane upon activation.
Protein Families Protein kinase superfamily, AGC Ser/Thr protein kinase family, RAC subfamily
Tissue Specificity Ubiquitously expressed. Present in ovary, where it is concentrated at the basal side of follicle cells.
Database Links

KEGG: dme:Dmel_CG4006

STRING: 7227.FBpp0082682

UniGene: Dm.1219

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