CD33 is a gene providing an instruction of making a protein named myeloid cell surface antigen CD33 (also abbreviated as CD33). CD33 protein, also known as SIGLEC3 or gp67, is a type I transmembrane protein exclusively expressed on immune cells, including myeloid stem cells, myeloblasts and monoblasts, monocytes/macrophages, granulocyte precursors, and mast cells. It is a 67kDa glycosylated transmembrane protein belonging to the Siglec family. After cross-linking or ligand binding, activated CD33 mediates inhibitory signals and regulates intracellular calcium mobilization, cell adhesion, leukemia cell apoptosis, myeloid cell maturation, and cytokine production.
During differentiation, CD33 is decreased on mature granulocytes but is retained on macrophages, monocytes and dendritic cells. CD33 has limited expression in non-hematopoietic tissues but is highly expressed in AML cells. In addition, CD33 has now been demonstrated to be expressed in leukemic progenitor cells, making this molecule a very promising therapeutic target for acute myelocytic leukemia (AML). At present, there are many companies developing monoclonal antibodies, double antibodies, tertiary antibodies, and ADCs targeting CD33, including Wyeth Laboratories, Seagen Inc., Sanofi, Johnson & Johnson, etc.
Recombinant Human Myeloid cell surface antigen CD33(CD33),partial (Active) (CSB-MP004925HU)
Validated Data
Greater than 95% as determined by SDS-PAGE.
Immobilized CD33 at 2 μg/ml can bind Anti-CD33 rabbit monoclonal antibody, the EC50 of human CD33 protein is 4.289- 5.312 ng/ml.
The following CD33 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CD33 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004925GA01HU | CD33 Antibody | Human | ELISA,WB,IHC,IF |
CSB-PA004925ESR2HU | CD33 Antibody | Human | ELISA, IHC |
CSB-PA004925ESR1HU | CD33 Antibody | Human | ELISA, WB, IHC |
CSB-PA006175 | CD33 Antibody | Human | WB, ELISA |
CSB-PA070366 | CD33 Antibody | Human,Mouse | ELISA,WB,IHC |
CSB-PA243594 | CD33 Antibody | Human | ELISA,WB,IHC |
CSB-PA004925LA01HU | CD33 Antibody | Human | ELISA, WB, IHC |
CSB-PA004925LD01HU | CD33 Antibody, Biotin conjugated | Human | ELISA |
CD33 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP730931MO CSB-EP730931MO CSB-BP730931MO CSB-MP730931MO CSB-EP730931MO-B |
Recombinant Mouse Myeloid cell surface antigen CD33 (Cd33), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD33 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-EP004925HU | Recombinant Human Myeloid cell surface antigen CD33 (CD33), partial | E.coli |
CSB-YP004925HU CSB-BP004925HU CSB-EP004925HU-B |
Recombinant Human Myeloid cell surface antigen CD33 (CD33), partial | Yeast Baculovirus In Vivo Biotinylation in E.coli |
CSB-CF004925HU(A4) | Recombinant Human Myeloid cell surface antigen CD33 (CD33) | in vitro E.coli expression system |
CSB-MP004925HU | Recombinant Human Myeloid cell surface antigen CD33 (CD33), partial (Active) | Mammalian cell |
CD33 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL004925HU | Human Myeloid cell surface antigen CD33(CD33) ELISA kit | serum, plasma, tissue homogenates | 31.25 pg/mL |