DNMT3A, also known as DNA methyltransferase 3α, has alternative names TBRS and HESJAS. DNMT3A is a member of the DNA methyltransferase family responsible for catalyzing de novo methylation of DNA, which involves adding a methyl group to the fifth carbon atom of cytosine in the DNA sequence. This process is crucial for gene expression regulation, embryonic development, imprinting, and X chromosome inactivation. The N-terminal PWWP domain of DNMT3A interacts with chromatin, regulating regions that can bind various transcription inhibitors, such as cofactor RP58, oncogene PML-RAR, or heterochromatin protein HP1B, and interacts with DNMT1 to activate histone deacetylase HDAC1, thereby promoting histone deacetylation and gene transcription repression. DNMT3A is closely associated with the initiation and progression of tumors; its genetic mutations can lead to abnormal methylation of genomic DNA, including reduced overall genomic DNA methylation levels, affecting gene expression and cellular function, and are closely linked to the development of various hematological disorders, including acute myeloid leukemia.
Recombinant Human DNA (cytosine-5)-methyltransferase 3A (DNMT3A) (CSB-EP897314HU(F3))
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
DNMT3A Antibody (CSB-PA007083LA01HU)
Validated Data
Immunohistochemistry of paraffin-embedded mouse liver tissue using CSB-PA007083LA01HU at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using CSB-PA007083LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
The following DNMT3A reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
DNMT3A Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA007083LA01HU | DNMT3A Antibody | Human | ELISA, IHC, IF |
CSB-PA007083GA01HU | DNMT3A Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA094007 | DNMT3A Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA632606 | DNMT3A Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA522891 | DNMT3A Antibody | Human,Mouse,Rat | ELISA,IHC |
DNMT3A Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP007083MO CSB-EP007083MO CSB-BP007083MO CSB-MP007083MO CSB-EP007083MO-B |
Recombinant Mouse DNA (cytosine-5)-methyltransferase 3A (Dnmt3a), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
DNMT3A Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP633132RA CSB-EP633132RA CSB-BP633132RA CSB-MP633132RA CSB-EP633132RA-B |
Recombinant Rat DNA (cytosine-5)-methyltransferase 3A (Dnmt3a), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
DNMT3A Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP690180CH CSB-EP690180CH CSB-BP690180CH CSB-MP690180CH CSB-EP690180CH-B |
Recombinant Chicken DNA (cytosine-5)-methyltransferase 3A (DNMT3A), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
DNMT3A Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP897314HU CSB-BP897314HU CSB-MP897314HU CSB-EP897314HU-B |
Recombinant Human DNA (cytosine-5)-methyltransferase 3A (DNMT3A), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP897314HU | Recombinant Human DNA (cytosine-5)-methyltransferase 3A (DNMT3A), partial | E.coli |
CSB-EP897314HU(F3) | Recombinant Human DNA (cytosine-5)-methyltransferase 3A (DNMT3A) | E.coli |