Code | CSB-E12107h |
Size | 96T,5×96T,10×96T |
Trial Size | ![]() |
Have Questions? | Leave a Message or Start an on-line Chat |
Description |
This Human NFKB1 ELISA Kit was designed for the quantitative measurement of Human NFKB1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL . |
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Target Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Alternative Names | DKFZp686C01211 ELISA Kit; DNA binding factor KBF1 ELISA Kit; DNA binding factor KBF1 EBP1 ELISA Kit; DNA-binding factor KBF1 ELISA Kit; EBP 1 ELISA Kit; EBP-1 ELISA Kit; EBP1 ELISA Kit; KBF1 ELISA Kit; MGC54151 ELISA Kit; nf b ELISA Kit; NF kappa B ELISA Kit; NF kappaB ELISA Kit; NF kappabeta ELISA Kit; NF kB1 ELISA Kit; NF-kappaB ELISA Kit; NFkappaB ELISA Kit; NFKB 1 ELISA Kit; NFKB p105 ELISA Kit; NFKB p50 ELISA Kit; NFKB-p105 ELISA Kit; Nfkb1 ELISA Kit; NFKB1_HUMAN ELISA Kit; Nuclear factor kappa B DNA binding subunit ELISA Kit; Nuclear factor kappa-B ELISA Kit; Nuclear factor kappa-B, subunit 1 ELISA Kit; Nuclear factor NF kappa B p105 subunit ELISA Kit; Nuclear factor NF kappa B p50 subunit ELISA Kit; Nuclear factor NF-kappa-B p50 subunit ELISA Kit; Nuclear factor of kappa light chain gene enhancer in B cells 1 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA Kit; p105 ELISA Kit; p50 ELISA Kit; p84/NF-kappa-B1 p98 ELISA Kit; Transcription factor NFKB1 ELISA Kit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Abbreviation | NFKB1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Uniprot No. | P19838 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species | Homo sapiens (Human) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sample Types | serum, plasma, tissue homogenates, cell lysates | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detection Range | 0.312 ng/mL-20 ng/mL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sensitivity | 0.078 ng/mL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Assay Time | 1-5h | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sample Volume | 50-100ul | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detection Wavelength | 450 nm | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Area | Cell Biology |
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Assay Principle | quantitative | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Measurement | Sandwich | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Precision |
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Linearity |
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Recovery |
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Typical Data |
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ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Troubleshooting and FAQs |
ELISA kit FAQs | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Storage | Store at 2-8°C. Please refer to protocol. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lead Time | 3-5 working days |
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I would like to know about human NFkB elisa kit
Is it possible to detect from serum probes of patients ?
I am doing my research in diabetic patients.
Function (From Uniprot) |
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
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Gene References into Functions |
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Involvement in disease | Immunodeficiency, common variable, 12 (CVID12) |
Subcellular Location | Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). |
Database Links |
HGNC: 7794 OMIM: 164011 KEGG: hsa:4790 STRING: 9606.ENSP00000226574 UniGene: Hs.618430 |