Human Nuclear factor-kappa B,NF-κB ELISA Kit

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Name: Human Nuclear factor-kappa B,NF-κB ELISA Kit
Brand: CUSABIO
SKU: CSB-E12107h

Product Details

Description

This Human NFKB1 ELISA Kit was designed for the quantitative measurement of Human NFKB1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

Target Name

nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Alternative Names

DKFZp686C01211 ELISA Kit; DNA binding factor KBF1 ELISA Kit; DNA binding factor KBF1 EBP1 ELISA Kit; DNA-binding factor KBF1 ELISA Kit; EBP 1 ELISA Kit; EBP-1 ELISA Kit; EBP1 ELISA Kit; KBF1 ELISA Kit; MGC54151 ELISA Kit; nf b ELISA Kit; NF kappa B ELISA Kit; NF kappaB ELISA Kit; NF kappabeta ELISA Kit; NF kB1 ELISA Kit; NF-kappaB ELISA Kit; NFkappaB ELISA Kit; NFKB 1 ELISA Kit; NFKB p105 ELISA Kit; NFKB p50 ELISA Kit; NFKB-p105 ELISA Kit; Nfkb1 ELISA Kit; NFKB1_HUMAN ELISA Kit; Nuclear factor kappa B DNA binding subunit ELISA Kit; Nuclear factor kappa-B ELISA Kit; Nuclear factor kappa-B, subunit 1 ELISA Kit; Nuclear factor NF kappa B p105 subunit ELISA Kit; Nuclear factor NF kappa B p50 subunit ELISA Kit; Nuclear factor NF-kappa-B p50 subunit ELISA Kit; Nuclear factor of kappa light chain gene enhancer in B cells 1 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA Kit; p105 ELISA Kit; p50 ELISA Kit; p84/NF-kappa-B1 p98 ELISA Kit; Transcription factor NFKB1 ELISA Kit

Abbreviation

NFKB1

Uniprot No.

P19838

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	93
	Range %	89-97
1:4	Average %	97
	Range %	91-103
1:8	Average %	90
	Range %	87-96

Recovery

The recovery of human NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	95	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.889	2.752	2.821	2.710
10	2.251	2.149	2.200	2.089
5	1.399	1.328	1.364	1.253
2.5	0.654	0.643	0.649	0.538
1.25	0.396	0.410	0.403	0.292
0.625	0.241	0.255	0.248	0.137
0.312	0.168	0.181	0.175	0.064
0	0.112	0.110	0.111

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Customer Reviews and Q&A

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■ Q&A

I would like to know about human NFkB elisa kit
Is it possible to detect from serum probes of patients ?
I am doing my research in diabetic patients.

Thanks for your inquiry.
CSB-E12107h is quantitative to determination the NF-κB.
The serum samples from diabetic patients can be detected with this kit. And the detection value,pls check your own actual result.
As for serum probes, do you make the research on molecular probes or other protein label probes（eg GFP)?
Generally, it can be detected if you do not change the NF-κB structure.Pls let me know if you have any further questions. Thank you.

Target Background

Function (From Uniprot)	NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
Gene References into Functions	NF-kappaB signaling contributes to prostate cancer cell proliferation and migration via androgen receptor and estrogen receptor beta. PMID: 30236540 PKC-delta isoform plays a crucial role in Tat-TLR4 signaling pathway to activate NF-kappaB and CXCL8 production. PMID: 28539656 Knockdown of cyclin dependent kinase inhibitor 2A (p16INK4A) in cardiac stem/progenitor cell (hCPC) reverses the senescent phenotype and has an antioxidant effect on aging hCPCs via NF-KAPPA B (NF-kB) signaling. PMID: 29675777 Chandipura virus infection triggered the activation of signalling pathways mediated by mitogen-activated protein kinases, including p38, JNK 1 and 2, and nuclear factor kappaB. PMID: 30001342 TSPAN15 interacts with BTRC to promote oesophageal squamous cell carcinoma metastasis via activating NF-kappaB signaling. PMID: 29650964 FABP5 promotes lipolysis of lipid droplets, de novo fatty acid synthesis and activation of NF-kappaB signaling in cancer cells. PMID: 29906613 Prognostic significance of NF-kappaB expression in non-small cell lung cancer PMID: 29813121 LMP1 functions to constitutively activate NF-kappaB signalling during nasopharynx cancer pathogenesis. PMID: 28098136 NF-kappaB signalling may repress ANT1 gene transcription and impair mitochondrial functions. PMID: 28317877 High NFKB expression is associated with chemotherapeutic resistance in gastric cancer. PMID: 30106453 PGF promotes epithelial-mesenchymal transition-like changes in retinal pigment epithelium cells under hypoxia by activating the NF-kappaB signaling pathway. PMID: 29769799 Data indicated that nestin regulated NF-kappa B (NF-kappaB) activity in foetal spinal cord tissues. PMID: 29697001 NF-kappaB p50 and NF-kappaB p65 in thyroid carcinoma were positively associated with tumour diameter and the presence of lymph node metastasis PMID: 30014762 This study establishes PML as an important regulator of NF-kappaB and demonstrates that PML-RARalpha dysregulates NF-kappaB. PMID: 28317833 Notch signaling can initiate Asb2 transcription and NF-kappa B activation in T cell acute lymphoblastic leukemia cells. PMID: 30116272 High NFKB expression is associated with colorectal cancer cell migration, invasion and metastasis PMID: 30015978 These findings indicated that microRNA-98 could promote apoptosis of glioma cells via inhibiting inhibitor of kappa B kinase epsilon/nuclear factor-kappa B signaling and presented a novel regulatory pathway of microRNA-98 by direct suppression of inhibitor of kappa B kinase epsilon/nuclear factor-kappa B expression in glioma cells. PMID: 29333957 Anti-rotavirus effect of TNF-alpha was achieved by NFkappaB-regulated genes via the activation of classical nuclear factor kappaB (NF-kappaB) signaling. PMID: 29859235 Knockdown of REG-GAMMA (REGgamma) may inhibit the proliferation and migration, and promote the apoptosis of plasma cell myeloma RPMI-8226 cells possibly by downregulating NF-kappa-B (NF-kappaB) signal pathway. PMID: 29020881 L5-LDL, a naturally occurring mild oxidized LDL, induced G-CSF and GM-CSF production in human macrophages through LOX-1, ERK2, and NF-kappaB dependent pathways PMID: 29078142 Priming cells with IFNbeta synergistically enhances IL6 induction in response to treatments that activate NF-kappaB, in a process that depends upon the recruitment of STAT2, IRF9. PMID: 29581268 HMGB1 promoted lung cancer invasion and metastasis by upregulating the expression and activity of MMP-2 in an NF-kappaB-dependent manner. PMID: 29850505 NF-kappaB activation in breast cancer cells depends on the presence of the CHORDC1 gene product Morgana. PMID: 29158506 Data suggest the angiopoietin-like 8 (ANGPTL8)/p62-IKKgamma axis as a negative feedback loop that regulates NF-kappaB activation, and extends the role of selective autophagy in fine-tuned inflammatory responses. PMID: 29255244 Studied role of bone marrow stromal cell antigen 2 (BST2) in gastric cancer (GC); results show BST2 is overexpressed in GC tissues and BST2 silencing inhibits cell proliferation and migration, partly by regulating NF-kappaB signaling. PMID: 29774441 vaspin decreased miR-33a levels, which in turn increased ABCA1 expression and cholesteorl efflux. PMID: 29653102 these results define a tumor-supportive role for CDCA3. PMID: 29627567 NFKB1 variants were significantly associated with type 2 diabetes PMID: 29601852 NF-kappaB has been identified as the main transcription factor regulating the induction of inflammation-related genes in intracranial aneurysms lesions. This transcription factor has also been related to intracranial aneurysms rupture and resulting Subarachnoid Hemorrhage. [review] PMID: 29671828 miR-150 predicts survival in patients with sepsis and inhibits lipopolysaccharide-induced inflammatory factors and apoptosis by targeting NF-kappaB1 in human umbilical vein endothelial cells. PMID: 29689269 These results illustrate an alternative mechanism of HIV-1 Vpr regulation of Kaposi's sarcoma-associated herpesvirus (KSHV) latency and aberrant cytokines through the miR-711/Notch/NF-kappaB axis. Our novel findings further demonstrate the role of an HIV-1-secreted regulatory protein in the KSHV life cycle and KSHV-related malignancies. PMID: 29976660 In conclusion, HSP70 modulates NF-kappaB activation in alveolar macrophages of TB patients, through inhibiting IkappaB-alpha phosphorylation or acting as a chaperon molecule to prevent NF-kappaB binding to the target genes by facilitating degradation. The upregulated HSP70 may suppress the release of pro-inflammatory cytokines during active pulmonary tuberculosis infection, and prevent overwhelming tissue damage. PMID: 28450725 Gene expression analyses show strong correlation between the cellular dynamic response and NF-kappaB-dependent target gene activation. PMID: 27381163 NF-kappaB served as a positive transcriptional regulator of WIP1 to activate its expression and affect its function in colorectal cancer cells. PMID: 29367109 Data do not support a role for the NFKB1 and HIF1A polymorphisms in the pathogenesis of bowel disease. PMID: 29307990 a few seconds of exposure to TNF is sufficient to activate the NF-kappaB pathway in HeLa cells and induce apoptotic cell death in both HeLa and Kym-1 cells PMID: 28004761 HMGB1 mediates fibroblast activity via RAGE-MAPK and NF-kappaB signaling in keloid scar formation. PMID: 29283384 High NFKB expression is associated with glioma. PMID: 28534933 NFkappaB1-94ins/ins genotype was associated with the risk of developing colorectal cancer in Egyptian subjects. PMID: 28389768 miR-146 exerted protective functions might be via up-regulation of Sirt1 thereby blocking NF-kappaB and Notch pathways. PMID: 29229881 Data suggest that environmental carcinogen PFOA (perfluorooctanoic acid) stimulates ovarian cancer cell migration, invasion, and MMP2/MMP9 expression by up-regulating ERK/NFkappaB signaling pathway. (MMP = matrix metallopeptidase; NFkappaB = nuclear factor kappa B) PMID: 29753068 High NFKB expression is associated with KSHV infection. PMID: 29698475 Significantly elevated blood levels of NFkappaB in myelodysplastic syndrome patients. PMID: 28856536 These data indicate a process of NF-kappaB-induced miR-506 suppression and JAG1 upregulation upon IL-1beta induction. PMID: 28926924 High Expressions of NFkappaB is associated with degenerative knee osteoarthritis. PMID: 28418842 Inflammatory factors suppress microRNA-1275 transcription in human adipocytes through NF-kappaB. PMID: 28901460 GSK-3beta is critically important for ordered NF-kappaB signalling through modulation of NEMO phosphorylation. PMID: 27929056 results establish a role for the linear Ubiquitin coat around cytosolic S. Typhimurium as the local NF-kappaB signalling platform and provide insights into the function of OTULIN in NF-kappaB activation during bacterial pathogenesis PMID: 28481361 the lymphotoxin beta receptor (LTbetaR) to elicit the fast release of NF-kappaB inducing kinase (NIK) from the receptor complex leading to non-canonical NF-kappaB signaling. PMID: 29329668 Data demonstrate that S. Typhimurium attenuates NF-kappaB signaling in fibroblasts; this tune-down in a central host defense might be instrumental for S. Typhimurium to establish intracellular persistent infections PMID: 27575017 Show More Hide All
Involvement in disease	Immunodeficiency, common variable, 12 (CVID12)
Subcellular Location	Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
Database Links	HGNC: 7794 OMIM: 164011 KEGG: hsa:4790 STRING: 9606.ENSP00000226574 UniGene: Hs.618430

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Check Code：

Code	CSB-E12107h
Size	96T,5×96T,10×96T
Trial Size
Have Questions?	Leave a Message or Start an on-line Chat

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.